Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"high-affinity antibodies"”.
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Extracellular Interaction Screening Using CRISPR Activation
… for a defined ligand. We show receptors for high-affinity antibodies and low-affinity ligands can be unambiguously identified when used in pools or as individual binding probes. We apply this technique to identify ligands for the adhesion G-protein-coupled receptors and show that the Nogo …
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Modelling the affinity maturation of B lymphocytes in the germinal centre
… development of a computer model to simulate the affinity maturation of B lymphocytes in the germinal centre in 3-D because certain behavioural aspects cannot currently be examined experimentally. The software tool recreates the structure of the germinal centre that enables the B cells to move and …
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STEPWISE DIFFERENTIATION OF IL21-PRODUCING FOLLICULAR HELPER T CELLS REVEALS DISTINCT DEVELOPMENTAL AND FUNCTIONAL STATES
… organs is essential for the formation of high affinity antibodies, a key effector mechanism of adaptive immunity. Interleukin (IL)-21 is a proinflammatory cytokine produced by Tfh cells, however the signals that license IL-21 production by these cells during their differentiation are …
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HIV Specific B Cell Response in Patients With Broadly Neutralizing Serum Activity
<p>Antibodies to conserved epitopes on the HIV surface protein gp140 can protect against infection in non-human primates, suggesting that vaccines that elicit such antibodies would be protective. Some HIV infected individuals develop high titers of broadly neutralizing IgG antibodies in their …
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Host-parasite interactions of barley yellow dwarf virus and oats: Physiological and immunological studies
… entails virus detection with strain-specific antibodies. Fourteen virus-specific monoclonal cell lines secreting antibodies (MAbs) directed to barley yellow dwarf virus (BYDV) strain MAV-NY were identified from two murine spleen fusions. All the monoclonals recognize native MAV, not …
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The Mechanism of cGAMP Induced Antitumor Effect and Its Application in Treating Cancer and Antibody Generation
… conjugate (ADC) that links a STING agonist to antibodies targeting EGFR, prevalent in many cancers. This STING ADC activates various immune cells and promotes macrophage polarization within tumors. Chapter Five utilizes cGAMP as an adjuvant to enhance the generation of SARS-CoV-2 antibodies, in …
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REGULATION OF ADAPTIVE IMMUNE RESPONSES TO PLASMODIUM CHABAUDI MALARIA INFECTION
… helper (Tfh) cells are essential for generating high-affinity antibodies for parasite clearance. Whereas the transcription factors Bcl6, Blimp-1, and STAT3 regulate the development of Tfh cells, T-bet and STAT4 direct the commitment of Th1 cells. Since our understanding of the regulation of …
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Elucidating the role of BCL6 in helper T cell activation, proliferation, and differentiation
… GC B cells is necessary for the development of high affinity antibodies specific for an invading pathogen. Germline BCL6-deficient mouse models limit our ability to study BCL6 function in T cells due to the strong inflammatory responses seen in these mice. To overcome this, our lab has developed …
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Development and Characterization of B7-H3-Targeted Radioimmunotherapies For The Treatment of Solid Tumors
… cell surface antigens using monoclonal antibodies. Radioimmunotherapy has been successful in treating hematologic cancers, such as non-Hodgkin’s Lymphoma. However, treatment of solid tumors by radioimmunotherapy remains a challenge, driven in part by a lack of high affinity antibodies …
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Systematic development of rationally designed antibodies targeting predetermined epitopes of interest and protein aggregation
… of aggregation-prone regions of proteins using antibodies, but a major challenge in establishing antibodies against pre-selected epitopes is the effectiveness of the target regions to behave as antigens. To address this problem, a method of rational design was recently established in our group …
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Determinants of antibody specificity
High throughput screening methods such as yeast surface display (YSD) are frequently able to isolate high affinity antibodies against clinical targets; however, the success of these candidates depends on selecting for both on-target binding and desirable biophysical characteristics. Development …
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Replaying Life's Tape With Intraclonal Germinal Center Evolution
<p>Because high-affinity antibodies produced by germinal centers (GCs) are critical in protecting against the ever-increasing array of pathogens, understanding how GC response allows our immune system to recognize a variety of pathogens and develop specific and effective immunity against each …
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Molecular modeling and simulations of viruses: structure, dynamics and antibody design
… capsids, to determine the atomic structure of highly-flexible capsid domains, which could not be solved using a single experimental method, and to unravel the molecular mechanisms of host-pathogen interactions. Furthermore, we have recently integrated MD simulation into a de novo antibody …