Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"high throughput screen"”.
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High throughput screen for cells with high extracellular metabolite consumption--secretion rates using microfluidic droplets
… is severely limited by the availability of high-throughput methods for selecting strains with significantly improved metabolite secretion or uptake rates. As soon as a metabolite is secreted, its association with the cell that secreted it is lost. Thus, each clone must grow in a separate …
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Development of a high throughput screen for the expression of membrane proteins and their purification and crystallisation
… the expression of membrane proteins requires screening for the best expression conditions. Purification of membrane proteins requires, due to their amphipathic character, the use of detergents to solubilise the membrane protein. The optimal combination of detergent and membrane protein is …
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Regulation of CYP3A5 in Pancreatic Ductal Adenocarcinoma
… expressed in normal tissues and expressed in high amounts in some cancers, including pancreatic ductal adenocarcinoma (PDAC). There have been several published studies regarding CYP3A5 regulators, but there is still much to be discovered concerning CYP3A5 regulation in cancer. To further …
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High-throughput screening for ADP-ribosyl hydrolase 3 inhibitors
… of PAR and MAR. Toward filling this need, a high-throughput screen was conducted using a fluorogenic substrate to discover an inhibitor of ARH3. A total of 496 primary hits have been identified and will be further studied to validate their ability to inhibit ARH3.
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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS
A highly specific fluorescent probe was discovered for mESC via high throughput screening of compounds. The hit compound was named CDy9. High throughput screen found 23 hit compounds that are specific to mESC. The cell panel test identified CDy9 as the most specific compound. FACS sorting of CDy9 …
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Using photonic crystal biosensors for pharmaceutical screening
… is explored. An ongoing project involving the screening of plant protein samples from Pakistan is then presented. In further work, the description of the photonic crystal biosensor used for a protein macromolecule binding assay is presented. A high-throughput screen aimed at identifying small …
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New Roles for the Integrated Stress Response in Cancer and Proteostasis
<p>The integrated stress response (ISR) is a highly conserved pathway that senses diverse stresses and responds by limiting total protein synthesis and redirecting translation to stress response transcripts. The ISR has the potential to modify a broad range of processes in cancer, but the major …
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Molecular analysis of insulin action using high throughput genetic screens
… cell line was developed and optimized for use in high throughput screening for regulators of insulin stimulated GLUT4 translocation. Insulin stimulation caused GLUT4 translocation to the plasma membrane, and the activation of the PI3K/Akt signaling pathway in these cells. Using this cell line, I …
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Design, Synthesis And Biological Evaluation Of Small Molecules That Inhibit Hiv-1 Entry And Sensitize Hiv-1 Infected Cells To Immune Responses
… biological evaluation. The second part highlights the discovery and development of a new structural class of small molecule CD4mc with the capacity to elicit ADCC responses that was identified through a high-throughput screen performed by our collaborator. This project focused on efforts …
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Phenothiazines Induce Abortive Autophagy Leading to Cancer Cell Death
… autophagy and drug treatments, we conducted a high-throughput drug screen to identify autophagy modulators. Our high-throughput screen utilized image based fluorescent microscopy for single cell analysis to identify chemical perturbants of the autophagic process. Phenothiazines emerged as the …
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The Discovery and Development of Thienopyrimidines as Inhibitors of Helicobacter pylori
… for the treatment of H. pylori infections, a high-throughput screen was performed to identify selective compounds against H. pylori. This screen revealed two selective and structurally related thienopyrimidines. Structure-activity relationship of the thienopyrimidines against H. pylori was …
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Preclinical Development of Therapeutic Strategies Against Triple-Negative and Inflammatory Breast Cancer
… MEK pathway. In breast cancer patients, low NOXA/high MCL1 tumor expression is indeed associated to poor survival outcomes, supporting the induction of NOXA expression, and subsequent inhibition of MCL1, for the treatment against TNBC and IBC.</p> <p>Secondly, we investigated the role of an …
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Functional Identification of MicroRNAs Counteracting Cellular Senescence
… of 879 human miRNAs mimics was used for a high throughput screen using high content microscopy to search for miRNAs able to restore a high replicative phenotype in WI-38 human diploid fibroblasts exhibiting replicative senescence. Screening endpoints were the analysis of the incorporation …
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Development of poly(ADP-ribose) glycohydrolase inhibitors and tetracyclic indoles as anticancer compounds
… tetracyclic indoles through a phenotypic screen. PARG plays a crucial role in DNA damage repair, and lack of PARG activity after DNA damage prevents repair and causes a build-up of toxic PAR chains. As such, PARG inhibitors have been pursued for decades as anticancer compounds. In this …
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Genetic and Chemical Genetic Approaches to Shiga Toxin Inhibition
… toxin pathogenesis. One approach relied on a high-throughput screen of a small compound library to identify potential inhibitors of Shiga toxin intracellular transport, as Shiga toxin is known to undergo a stepwise progression through host cells following endocytosis. This screen identified …
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Targeting Plasmodium Falciparum Dihydroorotate Dehydrogenase (PfDHODH) for the Development of New Antimalarials
… molecule identified through an in silico high-throughput screen by our group, exhibited moderate activity against this enzyme (IC50 = 0.91 ± 0.1 μM). Furthermore, the quinazolinone scaffold has not been previously explored for PfDHODH inhibition, thereby providing an opportunity to develop …
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The development of deoxynyboquinone as a personalized anticancer compound
… (DNQ) is a potent cytotoxin through a high-throughput screen, and we designed a concise and flexible synthesis of this molecule which enabled study of its mode of action. We found that DNQ generates toxic levels of reactive oxygen species (ROS) selectively in cancer cells through a …
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Drosophila melanogaster as a high-throughput and in vivo model to study body weight and feeding behaviour
… response to specific genetic perturbations. This screen was validated by its ability to detect metabolic perturbation in wild-type flies, and to differentiate between positive and negative control genes. Different Drosophila models were investigated for their suitability for the screen, and …
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Computational methodologies and resources for discovery of phosphorylation regulation and function in cellular networks
… analysis and repository resource for high-throughput quantitative measurements of post-translational modifications, called PTMScout. Additionally, we have developed a semi-automatic, high-throughput screen for unsupervised learning parameters based on their relative ability to …
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Biochemical Studies on Enzymatic Treatment of Pulp and Paper Mill Biosludge and Polymeric Lignin
… novel lignin-oxidizing enzymes, we developed a high-throughput screen for oxidase activity of purified proteins against polymeric lignin, where we screened 45 purified bacterial multicopper oxidases (MCOs) and heme-peroxidases and identified oxidase activity against polymeric lignin in 22 …
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