Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 140 for “"high content"”.
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OrganixInsights: High throughput imaging and high content screening of organoids
… and extracellular matrix interactions. However, high-resolution imaging of organoids generates large volumetric datasets that require advanced computational methods for analysis. This dissertation presents a comprehensive computational framework for high-content screening and quantitative …
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Study of altered metabolism in cancer by high content SRS microscopy
… perturb metabolic processes. Recently developed high-content, high-resolution SRS microscopy enables single-cell metabolic analysis and I successfully applied this approach to identify and target metabolic pathways in cancer therapy contexts.In the first project, I applied high-content SRS …
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Cross-Modality Profiling of High-Content Microscopy Images with Deep Learning
… to integrate metadata labels freely obtained in high-content screening into deep learning architectures. The use of automated microscopy in high-content phenotypic screening of cells treated with compounds or genetic perturbations produces a large amount of high-dimensional data. One example …
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Using high-content subcellular proteomics to identify x-ray-induced protein trafficking
… mechanisms to prevent damage or death. However, high levels of IR can overwhelm these mechanisms causing a cascade of events such as oxidative stress, DNA damage, cell cycle changes and cell death. The latter outcome is why IR has been used for radiotherapeutic treatments of highly proliferative …
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Methods and technologies for high-throughput and high-content small animal screening
High-throughput and high-content screening (HTS and HCS) of whole animals requires their immobilization for high-resolution imaging and manipulation. Here we present methods to enable HTS and HCS of the nematode Caenorhabditis elegans (C. elegans). First we present microfluidic technologies to …
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Multivariate methods for the statistical analysis of hyperdimensional high-content screening data
… increasingly complex datasets. In recent years, high-content screening (HCS) using RNA interference (RNAi) or other perturbation techniques in combination with automated microscopy has emerged as a promising investigative tool to explore intricate biological processes. Image-based HC screens …
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A NOVEL PIPELINE FOR DRUG DISCOVERY IN NEUROPSYCHIATRIC DISORDERS USING HIGH-CONTENT SINGLE-CELL SCREENING OF SIGNALLING NETWORK RESPONSES EX VIVO
… current work entails the development of a novel high content platform for the measurement of kinetic ligand responses across cell signalling networks at the single-cell level in distinct PBMC subtypes ex vivo. Using automated sample preparation, fluorescent cellular barcoding and flow cytometry …
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A high-content multiplexed screening platform for the evaluation and manipulation of force and fatigue of adult derived skeletal muscle myotubes in defined serum-free medium
… This engineered system has the potential for high-throughput screening of drugs for efficacy and drug toxicity studies as well as general biological studies on muscle fatigue.
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Low-Cost and High-Throughput Optofluidic Add-on Device for Light Sheet Imaging of Larval and Adult C. Elegans
… light sheet platform, allowing us to capture high-content cross-sectional images of nematodes' nervous systems at earlier stages of development and with higher throughputs. Our platform was put to the test in imaging larval and adult pan neural worms, as well as in analyzing control and …
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The effects of student field dependence on college students' ratings of instruction
… a lecture presentation in which (a) lecture content coverage and (b) instructor expressiveness (enthusiasm) were systematically manipulated. The four dependent variables were student scores representing: (a) a global rating of overall presentation, (b) specific ratings of lecture content, (c) …
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Using Chemical Probes to Examine Cellular Activities
… morphological markers of drug resistance via high-content microscopy. In the first part of my thesis, I use chemical probes to examine spastin's role in cell division. Spastin is a AAA (ATPases associated with diverse cellular activities) protein that is recruited in anaphase by the endosomal …
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Assessment of ion-selective optical nanosensors for drug screening applications
… nanosensors has recently been developed. This high-throughput, high-content system improves on the existing patch clamp and fluorescent dye technologies that presently dominate the ion-channel screening market. This paper introduces the nanosensor technology, reviews the current market for …
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Discovery of new biomarkers of mild cognitive impairment and Alzheimer's disease risk in buccal cells using laser scanning cytometry
… imaging and immunohistochemistry with high-content, multi-color fluorescence analysis, and can be used to identify specific cells in a heterogeneous population as well as scoring unique molecular events within them. This study aimed at investigating buccal cell types (buccal cell …
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An integrated approach to ciprofloxacin susceptibility analysis and high-throughput bacterial phenotyping in Salmonella
… inhibitory concentration (MIC). I have developed high-content imaging methodologies to screen Salmonella grown in the presence of ciprofloxacin. These morphological data suggest that there may be heterogeneous subpopulations with differential responses to ciprofloxacin, which was supported by …
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Polycondensation of Bridged Amino-Functionalized Trialkoxysilanes.
… which stabilized by organobridges and has high content aminon absorption site, can serve as a potential CO<sub>2</sub> adsorbent. Synthesis of such material is done by hydrolysis and polycondensation of trialkoxysilane. The fastest gelation was observed at reaction in the absence of acids …
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In vitro evaluation of Ultraviolet Radiation (UVR) exposure of the Retinal Pigmented Epithelium (RPE) and its relevance to Age-Related Macular Degeneration (AMD)
… toxicity. Finally, a cellpaint-like high content imaging assay was developed in order to, at once, quantify intracellular oxidative stress, nuclear morphology, mitochondrial stress, epithelial integrity and cell viability as part of a cellprofiling approach to quantifying UVR …
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Correlation of Heparan Sulfate Proteoglycan Structure and Metabolism With the Regulation of Cell Division
… all pools from the confluent cells exhibited a higher average charge density than the corresponding pools from the growing cells. Analysis of the mixtures of di- and tetrasaccharides formed by cleavage of HS with nitrous acid showed that the structural features of the HS in each pool were …
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Budding yeast cell cycle analysis and morphological characterization by automated image analysis
… yeast cell morphology and yeast cell cycle using high content screening with a high-capacity automated imaging system. The images obtained using this method can also provide information about fluorescently labelled proteins, unlike flow cytometry, which can only measure overall fluorescent …
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Improvement of Fluidization Ability for Group A Powders by Fine Powder Additives
… fluidization, which can be affected by the fine content. This project investigates the feasibility that employing the fine powders (≤ 20 μm) as additives to further improve the fluidization ability of Group A powders. Three additives, Coal-15, GB-6, and SiO2-5 are added into FCC-76, based on …
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Extraction of Heavy Metals from Aqueous Solutions using Chitosan/Montmorillonite Hybrid Hydrogels
… agent. Interestingly, the increment of MMT content also enhances the mechanical strength of the hydrogels. Adsorption study is performed with Cu(II) to evaluate the effects of pH and Cu(II) initial concentration. The maximum adsorption capacities at pH 3, 7 and 10 are 286, 282 and 179 mg/g, …
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