Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"high affinity site"”.
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Studies on the Mechanism of the Pyruvate Oxidase Flavoprotein of Escherichia Coli (Rapid Kinetics, Biochemical Software, Lipid-Activated Enzyme)
… other other amphiphilic substances bind to a high affinity site on the flavoenzyme and stimulate the activity about 25 fold. The enzyme can also be activated by controlled proteolysis of the reduced flavoenzyme, which destroys the high affinity lipid site.
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Synthesis and Characterization of 1-Methyl-4-Substituted-3-Piperidine-Carboxylic Acid N, N-Diethyamide.
… acid diethylamide (d-LSD) all act at the same site, namely, the serotonin (5-hydroxytryptamine) receptor in the brain, it was found that 1-methyl-1,2,5, 6-tetrahydropyridine-3-(N,N-diethylcarboxamide) (THPC), a compound similar in structure to the D-ring of LSD (Figure 1), blocked the …
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The effects of sodium cations on rabbit muscle enolase : evidence for the binding of 2PGA to apo-enolase with Na⁺
… interactions between the ligands and the active site residues, and to the interactions throughout the side chains of the secondary elements in the two monomers. However, surprisingly, in the absence of Mg$\sp{2+}$, 2PGA still protects the apo-enolase from inactivation even at high concentrations …
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Crystallographic studies of the metal-responsive transcription factor NikR
… RHH proteins are detailed in this thesis. The high- affinity nickel-binding site of NikR is located at the tetramer interface of the MBD, with square-planar coordination by three histidines and a cysteine sidechain. A structure of NikR in complex with a 30 base-pair oligonucleotide containing …
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Discovery of a Novel Metalloregular Family: Structure/Function Studies of E. Coli Rcnr
… damage or the occupation of non-native metal sites. Acquiring metal ions from the environment while limiting possible toxic effects requires the coordinated regulation of metal uptake, trafficking and efflux, which in bacteria is often carried out by metal-responsive transcription factors: …
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Hydrolysis and Toxicity of Aspartyl Peptides and the Influence of Metal Ions on Aspartyl Peptide Hydrolases in Salmonella Enterica Serovar Typhimurium
… experiments indicated that, of two metal binding sites of unequal affinity in DapE, Mn 2+ is bound to the low affinity site and Zn2+ to the high affinity site when DapE functions as a peptidase. Overexpression of DapE in the MPD dapE strain conferred strong growth on Asp-Leu as a Leu source …
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The role of Orc6 in ORC binding-site switching during helicase loading
… loading of two Mcm2-7 hexameric helicases in opposite orientations to form the Mcm2-7 double hexamer. These Mcm2-7 double hexamers mark sites of eventual bidirectional replication initiation upon entering S-phase, when activation of these helicases and recruitment of DNA polymerases leads to new …
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The G protein-coupling specificity of D2-like dopamine receptors
… ligand binding assays dopamine identified both high and low affinity states at each of the dopamine D2 receptor-G protein fusion proteins and the high affinity state was eliminated by guanine nucleotide. S-(-)-3PPP bound to an apparent single state of the constructs where the D2 receptor was …
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Intrinsic uncoupling in the ATP synthase of Escherichia coli. Studies on WT and ε-truncated mutants
… that the occupancy of ADP and Pi binding sites shifts the enzyme from a partially uncoupled state to a fully coupled state, and we expect that the ADP- and Pi-modulated intrinsic uncoupling is likely to be a general feature of prokaryotic ATP synthases. Moreover, the biphasicity of the …
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The interactions of cytochrome bo3 from Escherichia coli with its substrates - ubiquinone and oxygen
… a soluble quinol substrate, indicate one high affinity quinone binding site, which acts as a non-exchanging co-factor, and a second, low-affinity site which is the substrate site. The residues interacting with the quinone at the high affinity QH site are well defined through numerous …