Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"hiPSCs"”.
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Engineering hiPSCs to enhance neural progenitor transplantation in degenerative cervical myelopathy
Neural progenitor cell transplantation has been widely explored in traumatic spinal cord injury (SCI) but has yet to be evaluated as a treatment for degenerative cervical myelopathy (DCM), a non-traumatic form of spinal cord injury. Importantly, the extent of astrogliosis and extracellular matrix …
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Derivation of lymphocytes from human induced pluripotent stem cells
… human induced pluripotent stem cells (hiPSCs) have the advantage to provide a source of autologous transplantable blood cell populations suitable for treatment of patient specific hematological diseases. This research aims to derive human lymphocytes from hiPSCs. There are three …
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RNAi-based logic circuitry coupled to Cas9-mediated transcriptional control in human cells and applications to stem cell technology
… genome of human induced pluripotent stem cells (hiPSCs). This architecture can be theoretically programmed to precisely control stem cell differentiation. First, we use computational biology to analyze differential miRNA expression during human stem cell differentiation and guide biological …
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hiPSC-Derived Cerebral Small Vessel Disease Models
… using human induced pluripotent stem cells (hiPSCs) with COL4A1, COL4A2, and HTRA1 cSVD-related mutations to assess the shared role ECM plays in cSVD. These mutations induced tight junction abnormalities with Occludin discontinuity and Claudin-5 accumulation in hiPSCs-derived Brain …
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Generation of human inner ear organoids from pluripotent stem cells and an organ-on-chip platform
… the embryonic development of the inner ear from hiPSCs. However, our limited understanding of the microenvironmental niche in which human otic progenitors emerge and develop is a key barrier to progress. This project builds on our previous discovery of a 3D culture system that uses hiPSCs to …
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MULTISCALE TECHNOLOGIES FOR ENGINEERING AND CRYOPRESERVING OVARIAN TISSUES AND HUMAN IPSCs
… Human induced pluripotent stem cells (hiPSCs) hold tremendous potential for tissue engineering and cell-based medicine, as they have the capacity of differentiating into ectodermal, mesodermal, endodermal, and germ cell lineages. In recent years, research into differentiating hiPSCs …
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Development of a High-Throughput Human iPSC Chondrogenesis Platform and Applications for Arthritis Disease Modeling
… of human induced pluripotent stem cells (hiPSCs) to prescribed cell fates enables the engineering of patient-specific tissue types, such as hyaline cartilage, for applications in regenerative medicine, disease modeling, and drug screening. In many cases, however, these differentiation …
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Structural characterization of primary cilia using accelerated piezoelectrically driven STED nanoscopy
… implicated in maintenance of pluripotency of hiPSCs and their differentiation pathways toward various lineages, where primary cilia have been shown to play a critical role in mechano-chemical signaling across a wide spectrum of cell types. The functions of primary cilia in hiPSCs and their …
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Tailored peptide hydrogel-based three-dimensional system improves stem cell maintenance, therapeutic potential and differentiation
… of human induced pluripotent stem cells (hiPSCs), cell source is no longer an issue, but the maintenance of iPSC still poses challenges. Previously we developed a three-dimensional (3D) method that produces large amount of high quality hiPSCs, but the mechanism for 3D iPSC maintenance is …
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Engineering functional kidney tissue using human iPS cells
… enabled the derivation of renal organoids from hiPSCs; these organoids might be a powerful tool with important implications for regenerative medicine, but meticulous assessment of the functional abilities of the induced nephrons is key to the use of these organoids in any application. Here, I …
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Modeling Complex Neurological Disorders With Human Induced Pluripotent Stem Cells
… models. Human-induced pluripotent stem cells (HiPSCs), which can be differentiated into any cell type and capture the genetic variation across humans, may serve as a powerful model to elucidate the mechanisms behind such disorders. In this thesis we have explored mechanistic and cellular …
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Brain microvascular endothelial cell dysfunction in schizophrenia: a preliminary report
… BMECs. Human induced pluripotent stem cells (hiPSCs) provide an exciting new avenue for exploring the role of BMECs in SZ. We hypothesize that BMECs have intrinsic deficits that lead to BBB dysfunction in SZ. In this study, we first aimed to test whether the existing hiPSC-derived BMEC …
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Artificial neural network and precision genome engineering frameworks for genetic system engineering in mammalian cells
… of a genetically encoded miRNA sensor in hiPSCs and their differentiated derivatives, including derivatives generated using GATA6-mediated and Neurogenin-mediated directed differentiation. The sensor provides a fluorescent readout based on the activity of a specific miRNA with single-cell …
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Neural and Physical Niches Orchestrate Skeletal Remodelling and Promote Cartilage Regeneration
… and induced pluripotent stem cells (hiPSCs) have the potential to permanently restore damaged cartilage in arthritic joints, yet chondrocyte hypertrophy is a major barrier for translational therapy. With chondrogenic differentiation, hBMSCs/SSCs undergo hypertrophy <i>in vitro</i> and …
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Understanding the biological processes underpinning neurodegeneration in Xeroderma Pigmentosum
… stages of the directed differentiation of hiPSCs into neurons revealed an elevated endoplasmic reticulum stress response and dysfunction of the ubiquitin proteasome system (UPS) in XP neuronal models. I have also demonstrated an accumulation of multiple oxidised nucleosides, implicating …
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The use of human pluripotent stem cells to model HNF1B-associated diabetes
… system. Human induced pluripotent stem cells (hiPSCs), which are capable of self-renewal and can differentiate into any cell type, provide an advantageous alternative to model human developmental diseases. The aim of this project was to develop a hiPSC based model system to determine the …
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The regulation and function of mRNA cap methylation in pluripotency and differentiation
… is conserved amongst species as also hESCs and hiPSCs exhibit high levels of RNMT and RAM compared to fibroblasts. So far, RNMT and RAM were described as a complex in all cells lines examined and it was assumed that are similarly regulated, instead surprisingly, during in vitro neural …
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Integrative and Scalable Pipeline for Multi-omic Characterization of Biological Tissues
… from human induced pluripotent stem cells (hiPSCs), can be utilized. Regardless of the models used, a large-scale, unbiased study of the brain is yet to be achieved due to the vast complexity and high interconnectedness of the cells in the brain. For the unbiased and holistic approaches …
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The impact of cytotoxic chemotherapy on somatic mutation in normal human cells
… human induced pluripotent stem cells (hiPSCs) than in MGMT-wild-type hiPSCs, showing a different mutational signature depending on MGMT status. The sequencing of normal tissue samples from chemotherapy-treated patients showed that many of these drugs are also mutagenic *in vivo*, with …
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