Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 39 for “"hiPSC"”.
-
hiPSC-Derived Cerebral Small Vessel Disease Models
… using human induced pluripotent stem cells (hiPSCs) with COL4A1, COL4A2, and HTRA1 cSVD-related mutations to assess the shared role ECM plays in cSVD. These mutations induced tight junction abnormalities with Occludin discontinuity and Claudin-5 accumulation in hiPSCs-derived Brain …
-
Delivery of hiPSC Derived Cardiomyocytes via an Injectable Nanofibrous Gelatin Polyurethane Composite Scaffold for Heart Tissue Repair
… pluripotent stem cell-derived cardiomyocytes (hiPSC-CM), has shown promising results in treating ischemic cardiomyopathy and heart failure. However, poor cell retention has been hindering the translation of such therapies. This thesis hypothesized that delivering hiPSC-CM aggregates on an …
-
Establishment of a Novel Patient hiPSC-derived in vitro Blood-Brain Barrier Model of Collagen IV Small Vessel Disease
… to SVD, human induced pluripotent stem cells (hiPSC) generated from patients with mutations in COL4A1/2 genes (COL4A1G755R and COL4A2G702D), isogenic controls and multiple wild-type (WT) control lines were used to establish a novel in vitro BBB model of Collagen IV SVD. Encompassing …
-
Brain microvascular endothelial cell dysfunction in schizophrenia: a preliminary report
… BMECs. Human induced pluripotent stem cells (hiPSCs) provide an exciting new avenue for exploring the role of BMECs in SZ. We hypothesize that BMECs have intrinsic deficits that lead to BBB dysfunction in SZ. In this study, we first aimed to test whether the existing hiPSC-derived BMEC …
-
Modeling colorectal cancer initiation using human iPSC-derived intestinal organoids
OBJECTIVE: Human induced pluripotent stem cell (hiPSC)-derived human intestinal organoids (HIOs) are increasingly utilized as in-vitro models for studying colorectal cancer (CRC). Prevalent oncogenic drivers in CRC include mutations in EGFR (L858R), KRAS (G12D) and CTNNB1 (S33C) which are known to …
-
Effects of GATA4-targeted compounds in hypertrophic models of hypoplastic left heart syndrome cardiomyocytes
… kantasoluista erilaistetut sydänlihassolut (hiPSC-sydänlihassolut) eroavat terveistä luovuttajista johdetuista hiPSC-sydänlihassoluista stressivasteen suhteen altistamalla solut hypertrofisille ärsykkeille, nimittäin endoteliini-1:lle (ET-1) ja sykliselle mekaaniselle venytykselle. Lisäksi …
-
Engineered Heart Tissues for Advanced Disease Modeling
… pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) are a powerful, in vitro tool for investigating cardiac pathologies. Although hiPSC-CMs have a relatively immature phenotype compared to primary adult cardiomyocytes, their physiological relevance can be improved by incorporating them …
-
Stretch Injury Alters Calcium Dynamics and Actin Organization in Human Astrocytes
… how human induced pluripotent stem cell (hiPSC)- derived astrocytes respond to mechanical strain, aiming to reproduce changes in astrocyte behavior that can initiate neurodegeneration. In this research, a two- dimensional in vitro stretch model was used to apply controlled mechanical …
-
Development of a High-Throughput Human iPSC Chondrogenesis Platform and Applications for Arthritis Disease Modeling
… of human induced pluripotent stem cells (hiPSCs) to prescribed cell fates enables the engineering of patient-specific tissue types, such as hyaline cartilage, for applications in regenerative medicine, disease modeling, and drug screening. In many cases, however, these differentiation …
-
Engineering hiPSCs to enhance neural progenitor transplantation in degenerative cervical myelopathy
… it remains unclear whether forward reprogrammed hiPSC-derived glutamatergic and GABAergic neurons contain PNNs. The second aim of this thesis was to determine the extent of PNN formation in forward reprogrammed neurons. Both NGN2 (glutamatergic) and ASCL1/DLX2 (GABAergic) lines displayed …
-
Modeling Complex Neurological Disorders With Human Induced Pluripotent Stem Cells
… models. Human-induced pluripotent stem cells (HiPSCs), which can be differentiated into any cell type and capture the genetic variation across humans, may serve as a powerful model to elucidate the mechanisms behind such disorders. In this thesis we have explored mechanistic and cellular …
-
The use of human pluripotent stem cells to model HNF1B-associated diabetes
… system. Human induced pluripotent stem cells (hiPSCs), which are capable of self-renewal and can differentiate into any cell type, provide an advantageous alternative to model human developmental diseases. The aim of this project was to develop a hiPSC based model system to determine the …
-
Biochemical effects of organophosphorous compounds on cultured rat and human cardiomyocyte-like cells
… pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) were used for the validation of selected OP effects in a more human relevant system. The differentiation of both H9c2 and human cardiomyocytes resulted in increased expression of differentiated muscle markers such as troponin 1, …
-
Localizing Strain in a 2D in vitro Stretch System to Model Regional Injury Dynamics
… periphery. Human induced pluripotent stem cell (hiPSC)-derived astrocytes were cultured on polydopamine and Matrigel® coated PDMS membranes and subjected to the high-friction indentation. Calcein AM staining before and after injury revealed decreased cell viability in the periphery, and …
-
Characterisation of primary cilia in human hypothalamic neurons
… generating human-induced pluripotent stem cell (hiPSC) lines stably expressing a transmembrane construct that both enables fluorescently labelling of cilia and drives the expression of a peroxidase to allow the streptavidin pull-down and identification of ciliary proteins in human neurons. To …
-
Understanding the biological processes underpinning neurodegeneration in Xeroderma Pigmentosum
… groups using a human induced pluripotent stem (hiPSC) model. Functional multi-omic characterisation at several stages of the directed differentiation of hiPSCs into neurons revealed an elevated endoplasmic reticulum stress response and dysfunction of the ubiquitin proteasome system (UPS) in XP …
-
Novel roles of microtubule-associated tau in radiation-induced brain injury
… analysis of human induced pluripotent stem cell (hiPSC)-derived neurons subjected to radiation-induced injury revealed unique methionine viii oxidation sites within fetal tau peptide sequences. Curiously, the absence of insoluble tau in irradiated hiPSC-derived neurons hints at potential …
-
Tailored peptide hydrogel-based three-dimensional system improves stem cell maintenance, therapeutic potential and differentiation
… of human induced pluripotent stem cells (hiPSCs), cell source is no longer an issue, but the maintenance of iPSC still poses challenges. Previously we developed a three-dimensional (3D) method that produces large amount of high quality hiPSCs, but the mechanism for 3D iPSC maintenance is …
-
INVESTING THE ROLES OF E26 TRANSFORMATION-SPECIFIC HOMOLOGOUS FACTOR IN CYSTIC FIBROSIS LUNG DISEASE
… relies on human induced pluripotent stem cells (hiPSC)-derived Airways Epithelial Cells (AECs). We first explored two differentiation protocols that generate lung AECs as organoids and Air-Liquid-Interface (ALI) cultures and outlined their respective advantages and limitations. Then, using the …
Page 1 of 2