Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"heterotrimeric G Proteins"”.
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The Role of Phosducin-like Protein as a Co-chaperone with the Cytosolic Chaperonin Complex in Assembly of the G Protein βγ Subunit Dimer
… TCP-1 (CCT), and the βγ subunit dimer of heterotrimeric G proteins (Gβγ). Here we provide details obtained from cryo-electron microscopic and biochemical studies on the structure of the complex between the cytosolic chaperonin CCT and PhLP. Binding of PhLP to CCT occurs through only one of …
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The Role of G-Protein Signaling in Pathogenesis in Cercospora zeae-maydis
… mechanisms are not well understood. In fungi, heterotrimeric G-proteins consist of three subunits (α, β, and γ) and mediate responses to environmental stimuli. They regulate diverse functions, including nutrient detection, virulence, fungal development, conidiation, secondary metabolism, and …
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Localization of Goα in the Developing and Adult Mouse Brain
Heterotrimeric G proteins mediate signal transduction generated by hormone and neurotransmitters. Among G proteins, Go, a member of the Gi/o family, is the most abundant heterotrimeric G protein in the brain but the roles and effectors for the Go have not been clearly defined. As previously …
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Genetic Dissection of Heart Development in the Fruit Fly Drosophila Melanogaster
… heart disease. In addition, we found that the heterotrimeric G proteins Gbeta 13F and G-oalpha 47A together with the RGS (regulator of G protein signaling) protein Loco function in the same pathway as Ggamma 1 to regulate septate junction formation in cardial cells of the Drosophila heart. We …
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Novel interactions of the 5-HT₂ₐ and related receptors with intracellular signalling proteins
… interact with and signal through the trimeric G-proteins. The 5-hydroxytryptamine 2A receptor (5- HT₂ₐR) is a member ofthe group I family of rhodopsin-related GPCRs. The receptor is known to activate phospholipase C (PLC) via the heterotrimeric G proteins Gaq/n, but has been shown to also signal …
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Heterotrimeric G protein beta:gamma bound to a biologically active peptide : structural definition of a preferred protein interaction surface
Heterotrimeric G proteins transmit information within and throughout eukaryotic cells. These proteins serve crucial functions in diverse signaling pathways, including visual transduction and adenylyl cyclase regulation. Heterotrimers are composed of α, β, and γ subunits. Gα is a guanosine …
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Molecular Study of Gβγ Dependent Breast Cancer Cell Migration and Invasion
… receptors (GPCRs) convey signals mainly via heterotrimeric G proteins in the form of Gα-GTP and Gβγ subunits. Recent studies show that expression of Gβγ-sequestering peptide derived from the carboxyl terminus of β-adrenergic receptor kinase (βARKct), attenuates multiple oncogenic pathways, …
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Influence of retinal states on the development and maintenance of retinofugal projections
… Gβ5 is the fifth member of the β subfamily of heterotrimeric G proteins. Gβ5 binds and stabilizes the R7 family of regulators of G-protein signaling (RGS), which accelerate Gi/o GTP hydrolysis. Gβ5 -/- mice, which lack R7RGS activity, have malformed synapses in the outer plexiform layer (OPL) …
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Ric-8: un nuevo GEF con estructura tipo "armadillo"
… guanine nucleotide exchange activity (GEF) on heterotrimeric G-proteins, but with different Gα subunits specificities. To gain insight on the mechanisms involved in RIC-8 cellular functions it is essential to obtain some information about its structure. Therefore, the aim of this thesis was to …
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Unzipping Amyloid Fibrils: How a Novel Calcium-Binding Protein, NUCB1, Prevents the Formation of Amyloid Fibrils
… reports suggested that NUCB1 might interact with heterotrimeric G protein α-subunits. We show that dimeric Ca<sup>2+</sup>-free sNUCB1 binds to heterologously expressed Gαi1 and that Ca<sup>2+</sup>-binding inhibits this interaction. We further show that the binding of sNUCB1 to Gαi1 inhibits its …
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Computational and experimental investigation of G protein-coupled receptor signalling mechanisms
… Receptors (GPCRs) are 7-transmembrane domain proteins. These receptors translate extracellular stimuli into cellular responses through heterotrimeric G proteins and β-arrestin proteins. These receptors are of considerable therapeutic interest, targeted by over 500 drugs approved by the Food …
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Charakterisierung der gamma-Untereinheiten AGG1 und AGG2 der heterotrimeren G-Proteine aus Arabidopsis thaliana
Mit spezifischen Antikörpern gegen AGG1 und AGG2 konnte gezeigt werden, dass AGG1 in der Membranfraktion von Arabidopsis-Infloreszenz- und Wurzelextrakten vorhanden ist, AGG2 im Gegensatz dazu ausschließlich in der Membranfraktion von Wurzelextrakten. Immunpräzipitationsversuche zeigten, dass beide …
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RATE-LIMITING STEP OF CONE PHOTOTRANSDUCTION RECOVERY AND OGUCHI DISEASE MECHANISMS
… the exchange of GDP for GTP on the α subunit of heterotrimeric Gprotein called transducin. Activated transducin relieves inhibitory constraint on cGMP-PDE, leading to rapid hydrolysis of cGMP, closure of cGMP gated cation channels, and membrane hyperpolarization. In order for photoreceptor to be …
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Role of Chaperonin CCT in G-protein Biosynthesis
… and function to assist the folding of other proteins. All chaperonins consist of two stacked rings, which come together to form a central cavity where folding can take place. The eukaryotic cytosolic chaperonin containing TCP1 (CCT) is a member of the group II chaperonins, which are defined …
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Escape to the interior: Characterization of the cytosolic delivery of C-terminal cargo subdomains by the N-terminus of pasteurella multocida toxin and subsequent toxin-mediated downregulation of Gaq-PLCb1 signaling through depletion of Gaq from the plasma membrane
… by constitutively activating the a-subunit of heterotrimeric G-proteins. This dissertation will focus primarily on two major unanswered questions regarding PMT and its action on host cells. First major question: During translocation of PMT from late endosomes into the cytosol of host cells, …