Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"heterologous protein expression"”.
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Transient heterologous protein expression in green microalgae
Introduction: Recombinant proteins are produced using recombinant DNA technology and are widely used in numerous industrial applications, as well as innovative therapeutics. As the requirements of industries and pharma grow more complex chemically, traditional chemical engineering is, at times, …
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Development of a high throughput reporter system using β-Galactosidase in the yeast : Pichia Pastoris
… gaining acclamation for its capabili ties in heterologous protein expression. In contrast to other host organisms such as bacteria or mammalian cells, P. pastoris offers many advantages over its counterparts. For example, P. pastoris is cost-effective in that it can grow to high cell densities …
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Protein expression and glycosylation in CHO cells
… cell culture process depends on sufficient expression and correct glycosylation of the recombinant product. Low product titer and inconsistent protein glycosylation constitute two major problems frequently encountered in biopharmaceutical production. Using Chinese Hamster Ovary (CHO) cells …
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Synthetic biology approaches for engineering bacteria as living therapeutics
… strain design. This platform enables high protein expression levels from the genome of E. coli Nissle through the development of a genetic "landing pad" system and characterization of genetic regulators that can be controlled through the addition of chemical inducers. In my second approach, …
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Calcium homeostasis in Pichia pastoris
… to produce from milligram to gram quantities of protein. In this study, we examined the effects of calcium and magnesium on growth, heterologous protein expression, and calcium homeostasis in P. pastoris . The divalent cations calcium and magnesium are responsible for diverse roles in in the …
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Equine interferon-gamma and associated cytokines
Cytokines are small proteins or glycoproteins that mediate cellular growth and differentiation and regulate immune responses. Upon encounter with antigen, CD4+ T cells are able to influence the character of the immune response elicited through the expression of distinct types of cytokines. Thl …
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Intestinal delivery of heterologous peptides with lactobacilli
… lactobacilli were developed as a tool to deliver heterologous protein into the gastrointestinal tract. Lactobacillus spp. are a potential delivery vehicle for oral antigens because of their generally regarded as safe (GRAS) status, ability to persist in the acidic environment of human …
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Building a robust bacterial chassis for synthetic biology
… plasmids experienced higher metabolic burden. Heterologous protein expression in the stress hardened cells was analyzed to determine if the physiological changes influence protein expression. GFP was used for testing the protein expression. The stress hardened cells showed an approximate two …
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Thermodynamics of λ-PCR Primer Design and Effective Ribosome Binding Sites
… were included in the primer designs to test heterologous protein expression of the cyan fluorescent reporter with different RBS strengths. These RBS sequences were designed with an online tool, the RBS Calculator. A chimeric primer design procedure for λ-PCR was developed and shown to …
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Genomics approaches to exploit the biotechnological potential of marine sponge-derived Streptomyces spp. isolates
… sponge-derived Streptomyces isolate, followed by heterologous protein expression in an E. coli host, as demonstrated in Chapter 4. In Chapter 2, two of the first complete genomes from marine sponge-derived Streptomyces spp. isolates were determined, namely from Streptomyces sp. SM17 and …
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Spore-forming bacteria as pharmaceutical factories
… 2,6-dipicolinic acid (DPA) is necessary for protein stability, but investigation of its role is confounded with that of water content. Tests replacing DPA with chemical analogues were not successful, but led to the novel approach of doping spores with extra DPA. Various Bacillus species, in …