Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"heterologous protein"”.
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Transient heterologous protein expression in green microalgae
Introduction: Recombinant proteins are produced using recombinant DNA technology and are widely used in numerous industrial applications, as well as innovative therapeutics. As the requirements of industries and pharma grow more complex chemically, traditional chemical engineering is, at times, …
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Increasing Heterologous Protein Secretion From the Yeast S. Cerevisiae Through Manipulation of Cellular Redox Factors
… Several possible actors in the ER oxidative protein folding chain were identified and their effects on BPTI secretion measured. Overexpression of Ero1p was shown to increase BPTI secretion, while overexpression of flavin-containing monooxygenase (FMO) and Fun9p did not. Overexpression of two …
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Clonal selection and characterization of epigenetic variation in Pichia pastoris
Recombinant proteins produced by different host organisms have been broadly used as therapeutics. Considering the demand for large quantities of protein drugs, methods are needed to increase reactor titers in a timely and cost-effective manner. We used random chemical mutagenesis to modify a …
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Development of a high throughput reporter system using β-Galactosidase in the yeast : Pichia Pastoris
… gaining acclamation for its capabili ties in heterologous protein expression. In contrast to other host organisms such as bacteria or mammalian cells, P. pastoris offers many advantages over its counterparts. For example, P. pastoris is cost-effective in that it can grow to high cell densities …
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Intestinal delivery of heterologous peptides with lactobacilli
… lactobacilli were developed as a tool to deliver heterologous protein into the gastrointestinal tract. Lactobacillus spp. are a potential delivery vehicle for oral antigens because of their generally regarded as safe (GRAS) status, ability to persist in the acidic environment of human …
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Engineering robust yeast strains for the conversion of xylose derived from lignocellulosic biomass to xylitol
… was evaluated. The effect of over-expressed heterologous protein production on strain robustness and metabolism was also assayed. Our results revealed that the overexpressed XR failed to improve on the xylose reduction ability conferred to our strains, likely by their native GRE3 gene.
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A directed evolution approach to engineering recombinant protein production in S. cerevisiae
The continued success of protein therapeutics has put a strain on industry's ability to meet the large demand. Creating a more productive expression host for the manufacture of these proteins is a potential solution. Although heterologous proteins are frequently made in organisms as disparate as E. …
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Engineering geobacillus thermoglucosidasius for efficient production of fuels and chemicals
… (aprt) and the stage III sporulation protein AA (spoIIIAA). Their effect on improving ethanol production was verified by disruption of both genes. By comparison of two strains, 95A1 was a good ethanol producer and easily genetic engineered. G. thermoglucosidasius 95A1 was furtherly …
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Protein expression and glycosylation in CHO cells
… product. Low product titer and inconsistent protein glycosylation constitute two major problems frequently encountered in biopharmaceutical production. Using Chinese Hamster Ovary (CHO) cells expressing recombinant human interferon-gamma (IFN-y) as the model system, this thesis investigated …
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Engineering spores to display g protein-coupled receptors for directed evolution
… of deleterious substances from the outside. G protein-coupled receptors (GPCRs) are the membrane proteins, which transmit signals across the cell membrane. GPCRs are involved in almost every physiological process, and irregular control leads to pathological conditions. Therefore, they are major …
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Synthetic biology approaches for engineering bacteria as living therapeutics
… strain design. This platform enables high protein expression levels from the genome of E. coli Nissle through the development of a genetic "landing pad" system and characterization of genetic regulators that can be controlled through the addition of chemical inducers. In my second approach, …
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Calcium homeostasis in Pichia pastoris
… to produce from milligram to gram quantities of protein. In this study, we examined the effects of calcium and magnesium on growth, heterologous protein expression, and calcium homeostasis in P. pastoris . The divalent cations calcium and magnesium are responsible for diverse roles in in the …
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The effects of cis- and trans-acting mutations on recombinant protein secretion in Pichia pastoris
… research and industrial settings for recombinant protein expression due to the ease of genetically modifying its genome, its ability to grow to large densities in inexpensive media, and its capability to perform posttranslational modifications. Multiple tools such as the cis -acting factors MATα …
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Equine interferon-gamma and associated cytokines
Cytokines are small proteins or glycoproteins that mediate cellular growth and differentiation and regulate immune responses. Upon encounter with antigen, CD4+ T cells are able to influence the character of the immune response elicited through the expression of distinct types of cytokines. Thl …
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Identification and characterization of components that overcome secretion limitations of the yeast Pichia pastoris
… expression system commonly used to secrete heterologous protein. Although P. pastoris is a popular host organism, secretion inefficiency continues to be a major hurdle in its ability to produce high levels of foreign protein. Optimization of cis- and trans-acting factors has greatly enhanced …
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Development of Bacillus subtilis spores and cells for surface display of proteins
… developing efficient display systems expressing heterologous polypeptides on the surface of bioparticles such as phages, bacterial and eukaryotic cells and bacterial spores. Among these bioparticles, the endospore from B. subtilis has advantages, including feasibility of production, safety …
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ELUCIDATION OF MECHANISMS GENERATING 5-HYDROXYMETHYLCYTOSINE (5hmC) IN MAMMALIAN MITOCHONDRIA
… We are now pursuing the role of Tet proteins in generating 5hmC in mtDNA. Using an in vitro enzyme assay, we have successfully detected Tet activity in crude and percoll purified mitochondrial fractions of HCT116 cells. Mitoprot analysis on Tet family predicts that Tet1 may be …
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Functional consequences of cytosine methylation in mitochondrial DNA catalyzed by DNA methyltransferase 1
… across mammalian species, and directs a heterologous protein to the mitochondria. To investigate the function of mitochondrial DNMT1 (mtDNMT1), we created a cell line that carries a tandem-affinity purification (TAP) tag at the C-terminus of a single endogenous human DNMT1 allele. Using …
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Development of microalgae as a biopharming platform
… platform for the expression of recombinant protein. First it had to be established whether the available plant expression vectors could be used for the transfer of genetic material into packed microalgae. The method showed successful transfer of the neomycin phosphotransferase II resistance …
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An integrated approach to enable rapid scalable upstream production of subunit vaccines with Pichia pastoris (Komagataella phaffii)
… engineering appear to be generalizable to other proteins of interest. In the second part, we present a novel “modular blending” approach to media development. This new method enabled the design of a soluble medium with 2x higher productivity than our previous best defined production medium and …
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