Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"heterologous gene expression"”.
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Insect expression systems : improving intracellular and heterologous gene expression
Expression systems (ES) in a wide variety of biological systems are used to provide foreign protein. Protein production in mammalian cells is a labour-intensive and expensive process and insect cells have been used as cheaper alternatives. Insect ES are based on two types of vector; viral- and …
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Molecular Characterization of the Heat Shock-Regulatedgroesl Operon in Lactobacillus Zeae Strain 102S
… little is known about their physiological and genetic responses to environmental stresses. These studies were undertaken to evaluate the stress responses of a strain used in dairy fermentations in order to develop a valuable genetic tool, a regulated promoter for use in food-grade heterologous …
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Investigating Epigenetics as a Mechanism to Regulate Bacterial Heterologous Expression
Epigenetic DNA methylation in bacteria is involved in phage defense, and it can also affect gene transcription. Epigenetic methylation as part of restriction-modification systems is frequently considered a barrier in synthetic biology since it can prevent the introduction of heterologous genes to …
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CRISPR-based Control of Biosynthetic Pathways and Genetic Biosensors, Informed by a Quantitative Investigation of Metabolic Burden
The heterologous, engineered genetics often used for biosynthetic chemical production share resources with host gene expression. While the host’s endogenous expression has evolved along with complex regulatory networks to balance supply and demand, or cost and benefit, heterologous expression …
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The molecular characterisation of the recA locus in the opportunistic pathogen Bacteroides fragilis
… thesis was to characterise the B. fragilis recA gene cluster with respect to its genomic context and the transcriptional regulation of the genes in response to metronidazole and oxygen stress. The possible functional roles of the proteins encoded by these genes in protection against these …
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Ochrobactrum anthropi: a soil bacterium for the study of Brucella virulence
… elusive and some of the identified virulence genes are putative. Disruption of putative virulence genes and studying the consequent effect on attenuation in cell lines or mouse models is a widely used method. However, in most cases it is not apparent whether the mutated genes encode virulence …
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Engineering and characterization of single chain antibody fragments (scFvs) specific to key enzymes in polyamine biosynthesis and manipulation of polyamine pathway by constitutive expression of recombinant ODC and SDE enzymes in transgenic tobacco
… modulation of specific enzymatic activities via heterologous gene expression in transgenic plants. Previous studies have indicated that the polyamine pathway is tightly controlled in the end product accumulation. The objective of this study was to achieve changes in the pathway by combining …
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ApolipoproteinE-Sendai (Arg 145 ->Pro) : Expression, Reinigung und Charakterisierung einer Apolipoprotein E-Variante, die mit der Lipoprotein-Glomerulopathie assoziiert ist
Varianten des Apolipoprotein E(ApoE)sind in Zusammenhang mit der Lipoprotein-Glomerulopathie aufgetreten, einer neuen Nierenerkrankung, bei der es zur Ablagerung von Lipoproteinen in glomerulären Kapillaren kommt. Ein Drittel der betroffenen Patienten sind heterozygot für ApoE-Sendai(Arg145->Pro). …
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Development of a gene therapy approach for the treatment of human mammary carcinoma using tissue specific retroviral vectors
… cancer is commonly thought to be incurable, but gene therapy strategies with suicide genes are emerging as a potential treatment for metastatic breast cancer. Retroviral vectors are attractive candidates for such in vivo gene therapy applications. Most of these vectors contain viral promoters …