Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 147 for “"heterologous expression"”.
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Physiological effects of heterologous expression of proteorhodopsin photosystems
… supplementing the cellular energy system of a heterologous host, paving the way for future engineering applications in photoheterotrophy.
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Investigating Epigenetics as a Mechanism to Regulate Bacterial Heterologous Expression
… biology since it can prevent the introduction of heterologous genes to new host organisms. Due to this, many studies that construct bacterial hosts for use in synthetic biology often remove these restriction-modification systems. There are no studies however that have attempted to optimize …
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Homology Cloning, Heterologous Expression and Characterization of A New Channelrhodopsin
… of these new channelrhodopsins along with the expression of <em>Ca</em>ChR1 was published in Photochemistry and Photobiology in January 2012</p>
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Characterization and Heterologous Expression of Recombinant Drosophila melanogaster Fatty Acyl CoA Reductase
… very few of these FARs have been characterized. Expression of the FAR CG18031 from Drosophila melanogaster in Spodoptera frugiperda (Sf)9 cells will allow for functional characterization of the enzyme. Substrate and chain-length specificity can then be determined by gas chromatography analysis. …
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Cloning, Heterologous Expression, and Characterization of Human and Escherichia Coli Choline Dehydrogenases
Membrane bound choline dehydrogenase (CHDH) catalyzes the first committed step of choline oxidation to betaine-glycine in mammals and in some bacteria such as E. coli and P. aeruginosa . In mammals, it is an important source of one-carbon units for cytosolic folate- and AdoMet-dependent reactions, …
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Identifying Potential Agonists Of TRPA1 Using A Heterologous Expression System And Transgenic Mice
Chemical irritants stimulate the trigeminal nerve through many different receptor proteins, including TRP channels. TRPA1, a highly conserved TRP channel, is known to be activated by over ninety compounds. As TRPA1 is promiscuous in nature, it was considered a likely receptor for stimuli activating …
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The vesicular glutamate transporter (VGLUT): heterologous expression, proteoliposome, computational and mass spectral studies
<P>Vesicular glutamate transporters (VGLUTs) are integral membrane proteins that uptake glutamate into synaptic vesicles and are involved in glutamatergic neurotransmission. Since VGLUTs were identified and cloned, efforts have been made to characterize their functional roles. However, due to …
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The vesicular glutamate transporter (VGLUT): heterologous expression, proteoliposome, computational and mass spectral studies
<P>Vesicular glutamate transporters (VGLUTs) are integral membrane proteins that uptake glutamate into synaptic vesicles and are involved in glutamatergic neurotransmission. Since VGLUTs were identified and cloned, efforts have been made to characterize their functional roles. However, due to …
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Studies of the soluble methane monooxygenase : heterologous expression and reactions with nitric oxide
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1999.
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Heterologous Expression of Alpha 6*- Nicotinic Acetylcholine Receptors and the Natural Distribution of Alpha 6 Subunits
… immortalized cell lines were generated that heterologously express nAChR α6 subunits in combination with other nAChR subunits found in reward brain regions (nAChR subunit combinations α6β2, α6β4, α6β2β3, α6β4β3, α6β2β3α5, α6β4β3α5, α6α4β2β3 and α6α4β4β3). The α6α4β2β3 combination may have a …
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The characterization of two novel class II lanthipeptides from Thalassomonas viridans by heterologous expression in Escherichia coli
It is estimated that in 2022, 4.95 million people died from illnesses where antimicrobial resistance played a role, of which 1.27 million people died as a direct result of antimicrobial resistance. With the rising emergence of antibiotic-resistant microorganisms, there is an urgent need for the …
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The characterization of two novel class II lanthipeptides from thalassomonas viridans by heterologous expression in escherichia coli
It is estimated that in 2022, 4.95 million people died from illnesses where antimicrobial resistance played a role, of which 1.27 million people died as a direct result of antimicrobial resistance. With the rising emergence of antibiotic-resistant microorganisms, there is an urgent need for the …
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Heterologous expression of alcohol acetyltransferase genes in Escherichia coli and Clostridium acetobutylicum for the production of esters
This thesis focuses on the heterologous expression of alcohol acetyltransferase (AATase) genes in Escherichia coli and Clostridium acetobutylicum for the production of the esters isoamyl acetate, butyl acetate and butyl butyrate. Isoamyl acetate, butyl acetate and butyl butyrate are esters that …
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Heterologous Expression of Arabidopsis Thaliana Purple Acid Phosphatase Gene (Atpap15) in Crops for Phytoremediation of Sites Contaminated with Excess Phosphorus
… selection markers. Confirmation of mGFP expression in the transformed alfalfa seedling was performed using Zeiss microscope fitted with GFP filters. Results showed that this protocol is replicable and efficient. In planta transformation method, therefore, may be used for the development …
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Cloning, Heterologous Expression in Yeast, and Biochemical Characterization of Recombinant Putative Glucosyltransferase Clones 9 and 11 from Grapefruit (Citrus paradisi)
… into pPICZA for transformation into yeast, expression, and subsequent characterization.</p>
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Dual-omics approach for 3,4-dihydroisocoumarin characterisation from Hydrangea serrata and isolation of polyketide synthases for heterologous expression in Pseudomonas putida KT2440
… stilbenoid biosynthesis and changes the gene expression according to other studies. The effect on H. serrata Oamacha was analysed in this work on the level of metabolite contents and differential gene expression. A de-novo transcriptome assembly of leaves and roots alongside with functional …
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Acclimatization to High-Altitude, Long-Term Hypoxia Alters BK Channel Structure and Function
… groups. These mechanisms included: differential expression of the accessary BK -1 subunit; differential phosphorylation of the BK subunit; and splice variation of the BK subunit. Using molecular cloning, heterologous expression, and patch-clamp electrophysiology techniques, we elucidated a …
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Studies on Structure and Dynamics of Components of Phytochrome Mediated Light Signaling in Plants and Cyanobacteria
… have been solved. This could be accomplished by heterologous expression, purification and crystallization of the proteins, and X-ray structure determination, employing multiple anomalous wavelength dispersion (MAD) and molecular replacement (MR) techniques.
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