Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"heterologe Genexpression"”.
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Optimierung von Schizosaccharomyces pombe für die heterologe Genexpression
… deutlich erhöhten Ausbeute des disulfidhaltigen heterologen Proteins Orf19p-HA führt. Dieser Befund impliziert, dass in S. pombe die Oxidation der Disulfidbrücken für die Faltung von Proteinen vermutlich limitierend ist.
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Entwicklung von Fermentationsprozessen zur Produktion rekombinanter Antikörperfragmente in Pichia pastoris und Nicotiana tabacum
Being partly funded by the EU-project "Processing technology for recovery of recombinant antibody produced in crop plants" (FAIR-PL-CT96-1039), the aim of this work was to provide approximately 500 mg of the recombinant single-chain antibody fragment 4813 to enable the development of a synthetic …
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Rekombinante Herstellung und Charakterisierung phenoloxidierender Enzyme aus Geobacillus stearothermophilus zur Evaluierung einer biosensorischen Anwendung
In the current thesis the genetic structure of the phenol hydroxylase of Geobacillus stearothermophilus has been clarified. The single components Phe A1 (oxygenase component), Phe A2 (Flavin reductase component) and a tandem construct consisting of both components were successfully produced with an …
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Entwicklung eines Enzym-Modul-Systems zur Synthese und in situ Regeneration von dTDP-aktivierten Desoxyzuckern
The results of this thesis showed for the first time that it is possible to produce glycosylated natural compounds in vitro with in situ regeneration of dTDP-activated deoxysugars. The deoxysugar biosynthesis genes from the avilamycine- (aviS, aviT), urdamycine- (urdR) and midekamycine-biosynthesis …
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Untersuchungen zur metabolismus-bedingten Insektizid-Resistenz von Drosophila melanogaster als Beispiel für den Einsatz Cytochrom P450-transgener Zellsuspensionskulturen von Nicotiana tabacum zur Aufklärung der enzymatischen Kapazität des Ursprungsorganismus
With the worldwide use of insecticides, the number of resistant insect species increases resulting in decreased efficacy of pesticides and threat of human welfare by crop failures and disease transmission. Environmental concentrations of some insecticides are so high that even populations of …