Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"heteroduplex mobility assay"”.
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Genetic diversity of subtype C HIV-1 env variants in peripheral blood mononuclear cell (PBMC) DNA from infected mother-child pairs : a comparison of Heteroduplex Mobility Assay (HMA) and Base Excision Sequence Scanning (BESS) methods
… DNA differences were compared: I-leteroduplex Mobility Assay (I-IMA) and Base Excision Sequence Scanning (BESS). These methods were validated using sequence data. Extracted PBMC DNA from infected mother-child pairs were used to amplify the V3 region by nested PCR. DNA fragments were cloned into …
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Analysis of the role of nuclear factor-kappa B in insulin resistance caused by antiretroviral drugs
… treatment causes insulin resistance. Heteroduplex mobility assay has shown that CRISPR-Cas9 knock out the gene of interest. These results have played a foundation in understanding how CRISPR-Cas9 can be integrated and utilized in medical research.
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Human immunodeficiency virus type-1 distribution in South Africa and the relevance of genetic diversity on vaccine design
… Bloemfontein (n=4). Isolates were subtyped using heteroduplex mobility assay (HMA) based on the V3-V5 region of the env geneusing reference plasmids (2 B, 2 C and 1 D) representative of local subtypes. HMA identified four env subtypes: A, B, C and D. Subtype B viruses were found in 92.9% (26/28) …
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Molecular characterization of the major envelope protein of porcine reproductive and respiratory syndrome virus (PRRSV) and evaluation of its use for a diagnostic assay, vaccine development, and the examination of quasispecies evolution
… of PRRSV through genetic characterization, assay development, and quasispecies evaluation using the PRRSV ORF5 gene while also attempting to develop an improved PRRS vaccine. To explore the possible mechanism for the emergence of acute PRRS, the open reading frame 5 (ORF5) gene encoding the …
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Evolution of viral populations in individuals infected with single and multiple HIV subtypes : a study of HIV-1 dual infection in a high risk cohort of female bar workers from Tanzania
… infection using the multi region hybridization assay (MHA); secondly to investigate the dynamics of viral evolution among dually infected individuals using a gel based screening assay (heteroduplex mobility assay, HMA) and sequencing, lastly the study aimed to investigate the role of point …
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Cross-Species Infection and Characterization of Avian Hepatitis E Virus
… for further molecular characterization. Two heteroduplex mobility assays (HMA) were developed to genetically differentiate field strains of swine HEV and avian HEV from known reference strains. It was shown that the HMA profiles generally correlate well with nucleotide sequence identities and …