Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 17 of 17 for “"hepatoma cell lines"”.
-
Regulation of mitochondrial respiration by LDHB suppression in hepatoma cell lines
… and mitochondrial respiratory defects in hepatoma cells. In this study, we aimed to investigate how LDHB suppression is linked with mitochondrial respiratory dysfunction. When LDHB expression was suppressed by siRNA-mediated knockdown, cellular oxygen consumption rate was significantly …
-
Hepatitis B Virion and cccDNA Formation in Primary Tupaia Hepatocytes and Human Hepatoma Cell Lines upon HBV Genome Transduction with Replication-defective Adenovirus Vectors and in vivo Infection of Tupaias
… with factors on the surface and inside the host cell. To by-pass restrictions during the initial entry phase, recombinant replication-defective adenovirus vectors, either without or with a green fluorescent protein marker gene, were constructed and used to deliver complete HBV genomes into …
-
Roles of Interferon Lambda on Hepatitis B Virus Replication
… described IFN-λ family utilizing its distinct cellular receptor has been reported to exert inhibitory effect on replication of several viruses. In this study, I wanted to know whether IFN-λ1 inhibits HBV replication in human hepatoma cell lines and whether HBV modulates expression of CRF2-12, a …
-
Coordinated Control of Liver-Specific Genes in Cultured Cells from Mice and Rats
… fresh livers, primary hepatocyte cultures, and hepatoma cell lines, derived from mice or rats. The transcription rates of many different liver-specific but no common mRNAs were found to decline sharply within 24 hours when hepatocytes from the liver were explanted and placed into primary …
-
Do Elevated Levels of Glutathione Decrease the Toxicity of Plasticizers in Mouse Liver Cells?
… aims to investigate the response of two mouse hepatoma cell lines to the effects of DEHP and its bioactive metabolite mono (2-ethylhexyl) phthalate (MEHP). Cells were exposed to four concentrations (10, 50, 100, and 300 µM) of DEHP and MEHP, which are consistent with levels encountered in …
-
Preneoplastic hepatocyte hypothyroidism underlies hepatocellular carcinoma progression
Thyroid hormones elicit many cellular and metabolic effects. Most of them are mediated by the thyroid hormone 3,5,3’-L-triiodothyronine (T3) nuclear receptors (THRs), which act as transcription factors. THRs have also been implicated in tumorigenesis, although it is unclear whether they act as …
-
The Intracellular Changes and the Localization of Core particles by Hepatitis B Virus P Protein-Deficient Mutant
… pregenomic RNA (pgRNA). In this study, the intracellular changes of P protein-deficient mutant expressing stable cells and the localization of P protein-deficient mutant core particles were observed. MATERIALS & METHODS: HBV replicating and non-replicating stable cells had been established in …
-
Development and analysis of an in vitro model of inflammatory cytokine-mediated idiosyncratic drug hepatotoxicity
… both directly and indirectly, in drug effects on cellular physiology. Our ultimate goal is to develop an in vitro model effectively mimicking certain relevant aspects of the in vivo response of the human liver. In our initial effort described herein, we have designed a novel cell-based system …
-
Relationship between Hepatitis B Virus Core Particles and Intracellular Motility
… was observed in HBV replicating stable cell lines. Also, how HBV core particles were transported in cytoplasm was elucidated. MATERIALS & METHODS: HBV replicating stable cell lines were established in hepatoma cell lines, HuH7 and HepG2 cells. Using Northern blot and Southern blot …
-
Studies on Taxol Resistance Induced by HBx Viral Oncoprotein
… taxol often fails due to resistance of hepatocellular carcinoma (HCC). I attempted to determine the effect of taxolon HCC.Infection with hepatitis B virus (HBV)is a high risk factor for the development of HCC.Hepatitis B virus X (HBx)protein encoded by HBV genome is likely to play a important …
-
Correlation of Heparan Sulfate Proteoglycan Structure and Metabolism With the Regulation of Cell Division
Heparan sulfate proteoglycan (HSPG), a ubiquitous cell surface component in animal cells, is a complex, polyanionic macromolecule consisting of a core protein to which are attached several heparan sulfate (HS) chains. Growing and confluent cultures of a rat hepatocyte cell line were labeled with …
-
Albumin Transcriptional Regulation
… proteins and others of a more ubiquitous cellular distribution. It is therefore, the interaction of several factors (only some of which are liver specific or enriched) which dictates the liver specificity of this promoter. The albumin enhancer element was localized much farther upstream …
-
Expression and function of osteopontin variants in HCV-related liver disease and hepatocellular carcinoma.
… While OPN is significantly expressed in hepatocellular carcinoma (HCC) little is known as to its role and if it is expressed in the pre-cancerous hepatitis C virus (HCV) infected liver. In this thesis we show that OPN is expressed in the liver and in HCC as three variants, the full-length …
-
Expression and Functional Analysis of Hepatitis B Virus DNA Polymerase
… except polymerase, were cotransfected to hepatoma cells, all chimeras were incompatible for replication. Interestingly core particle formation varied in chimeric DNA polymerase construct transfected cells. This result suggests that the primary sequence of chimeric DNA polymerase may affect …
-
Regulatory Mechanisms of Mitotic Catastrophe Induced by Doxorubicin
… II, free radical generation, or damage to cell membranes and induce apoptosis, senescence or mitotic catastrophe. Because low dose of doxorubicin induce senescence or mitotic cell death, we attempted to investigate the possible link between the drug-induced senescence and mitotic cell death …
-
REGULATION OF CIRCADIAN CLOCKS AND METABOLISM BY ARYL HYDROCARBON RECEPTOR
… the expression of Per1 in mouse liver and hepatoma cell lines, respectively. Mechanistically, the disruption of the rhythm and the inhibition of Per1 expression occur secondary to the interaction between AhR and BMAL1, which attenuates transcriptional activity of the core clock complex …
-
Nanosized systems for efficient delivery of antitumoral and anti-inflammatory drugs
… Triiodothyronine (T3) delivery to hepatic cancer cells and nanosuspensions were proposed to enhance dermal bioavailability of Diclofenac acid, a potent nonsteroidal anti-inflammatory drug (NSAID) with a very low aqueous solubility. T3, is a thyroid hormone normally synthesized and secreted by the …