Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"hepatoma cell line"”.
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Regulation of Phospholipid Transfer Protein Secretion and Gene Expression in the Hepatoma Cell Line, HepG2
Hep G2 cells were used as a model to study HDL distribution and interconversion corresponding to variations in PLTP activity. An increase in PLTP activity by butyrate treatment was accompanied by increases in the amount of HDL having a hydrated density of 1.10 g/ml, HDL particle size and the …
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MOLECULAR BIOLOGY OF HEPATITIS B VIRUS DNA-CELL INTERACTION
… a ?gtll expression library constructed from the hepatoma cell line HuH-6 which was screened with a binding site probe derived from EII. Sequence analysis of E2BP revealed 86.6% homology with a rat hnRNP-C c-terminal protein sequence while conformational studies suggest a "helix-loop-helix'' motif …
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Mechanisms of action for aryl hydrocarbon receptor ligands in the PLHC-1 cell line
… and activity was characterized in the teleost hepatoma cell line, PLHC-1. This work was carried out in order to gain insights into mechanisms of halogenated aromatic hydrocarbon (HAH) toxicity. The results improve our ability to characterize the risks posed by HAH exposure as well as further …
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Regulation of Cholesterol Biosynthesis in Hepatocytes
… natural compounds were screened using the human hepatoma cell line HepG2. One compound, berberine, showed great potential as a regulator of cholesterol synthesis and so became the subject of this investigation. Berberine inhibited HMG-CoA reductase activity and decreased cellular accumulation of …
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Development and analysis of an in vitro model of inflammatory cytokine-mediated idiosyncratic drug hepatotoxicity
… both directly and indirectly, in drug effects on cellular physiology. Our ultimate goal is to develop an in vitro model effectively mimicking certain relevant aspects of the in vivo response of the human liver. In our initial effort described herein, we have designed a novel cell-based system …
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Einfluss von metabolisch wirksamen Hormonen auf die IL-6-induzierte Signaltransduktion
… In this work it could be shown both in the human hepatoma cell line HepG2 and in primary murine hepatocytes that glucocorticoids interfere with the IL-6-induced expression of the SOCS3 feedback inhibitor, thereby leading to an upregulation of IL-6-induced gene expression. Impaired transactivation …
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Herstellung und Charakterisierung einer induzierbaren Form des Transkriptionsfaktors FOXO1a
… the C-terminus of FOXO1a (FOXO1a-ER). In HEP-G2-cells transiently transfected with plasmids encoding the FOXO1a-ER fusion protein and a glucose-6-phosphatase reporter construct, hydroxytamoxifen induced a marked induction of glucose-6-phosphatase promoter activity, whereas no effect was observed …
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Phenotypic characterization of a clinical HBV/G isolate relative to a co-infecting HBV/A strain and HBV/A/G recombinant strains
… and transiently transfected into the HepG2 human hepatoma cell line for phenotypic analysis of each strain. HBV/G, HBV/A and HBV/A/G recombinant strains were isolated from Patient 1, while a different HBV/A/G recombinant strain was isolated from Patient 2. HBV replication capacity was measured …
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Hormetic dietary phytochemicals from Western Canadian plants: Identification, characterization and mechanistic insights
… from Western Canada for QR induction in a mouse hepatoma cell line (Hepa-1c1c7) led to the identification of twenty-one extracts capable of doubling the activity of QR. Bioassay-guided fractionation of six extracts led to the identification of novel classes of compounds with QR-inducing activity …
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Molecular responses of iron regulatory proteins to iron overload in human liver
… a known regulator of iron uptake and storage in cells, were found to be consistently down-regulated in the liver of patients with HFE-related HH and also in a human hepatoma cell line exposed to ferric ammonium citrate. The intracellular localisation of the protein was also studied and in …
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Leber-Fibrose: Erstellung eines Modells zur Analyse lebertoxischer Substanzen und Analyse der ltbp1-abhängigen Aktivierung hepatischer Sternzellen
… in vitro differentiated murine embryonic stem cells to generate functional cells for toxicological screenings. After validation of the isolation method (MACS), a reporter construct with an albumin promotor driven expression of the surface-/selection-marker was cloned and the expression …
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Mammalian cell cultures as a model system for the determination of cytotoxicity induced by cholesterol oxidation products
… origin. Oxysterols are known to be cyctotoxic to cells in culture and mode of toxicity has been identified as apoptosis in certain cell lines. The cytotoxicity of the oxysterols 25-hydroxycholesterol (25-OH) and 7β-hydroxycholesterol (7β-OH) was examined in two human cell lines, HepG2, a hepatoma …
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Identifizierung und funktionelle Analyse der humanen Xylosyltransferase I- und II-Promotoren
… Both XT isoforms represent key enzymes in extracellular matrix assembly and remodeling and do also play a major role in various pathological processes. The xylosyltransferases show a high degree of homology in structure and function. Nevertheless, they have divergent expression levels in …
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Mechanisms into the development of fatty liver disease: role of bile acids, Betaine and MHY908
… and a PPARα/γ dual agonist MHY908. Method: Human hepatoma cell line, VL17A cells were treated with saturated fatty acid palmitic acid (PA) with a range of concentrations between 10 μM - 300 μM for 24 h, 48 h and 72 h after which lipid accumulation, cell toxicity, oxidative stress and mitochondrial …
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Mechanisms into the development of fatty liver disease: role of free fatty acids and alcohol
… or in the presence of alcohol. Method: Human hepatoma cell line, VL-17A cells were treated with individual fatty acids (Palmitic (PA), Stearic (SA), Linoleic (LA), alpha-Linolenic (ALA), Arachidonic (AA) and Docosahexaenoic (DHA) acids) with various concentrations ranging between 0.5 μM to 300 …
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Isolation and characterisation of anti-diabetic pharmacological activities of phytoestrogens and components of Moringa peregrina (Forssk) Fiori
… six extracts on the uptake of glucose in a human hepatoma cell line (HepG2) using a well-studied fluorescent derivative of glucose, 2-2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino glucose (2-NBDG). The cells can take up 2-NBDG instead of glucose via glucose transporters and its uptake is …
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Initial Stages of Hepatitis B Virus Replication
PART 1 Intracellular Trafficking of HBV Core Particle in Various Stage of DNA Replication Inside of hepatitis B virus (HBV) core particle, pgRNA is reverse transcribed by HBV DNA polymerase into minus-strand DNA and pregenomic RNA (pgRNA) is degraded concomitantly by RNase H activity of DNA …
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Studies of the interaction of hepatitis B virus with human haemopoietic cells.
… of hepatitis B virus (HBV) with haemopoietic cells and cell lines, to address the reported tropism of HBV for haemopoietic tissues. Emphasis was directed at demonstrating specific attachment of HBV to defined subpopulations of peripheral blood leucocytes (PBL) and bone marrow cells (BM), and …
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Understanding the Role of the Hypervariable Region in the Open Reading Frame 1 of the Hepatitis E virus in Viral Replication
… understood due to the lack of an efficient cell culture system. Animal model systems, including non-human primates, swine, and chickens are being used to study some fundamental aspects of the HEV biology. Recently, novel animal strains of rat and rabbit HEV have been discovered, and whose …