Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"hepatic stellate cells"”.
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TGF-beta,Smad signaling in hepatic stellate cells and during liver fibrogenesis
… growth factor (TGF)-beta is a key mediator of hepatic stellate cell (HSC) activation and extracellular matrix accumulation leading to fibrosis. Aim of the present study was to perturb the TGF-beta pathway in this process by overexpression of Smad7, an intracellular antagonist of TGF-beta …
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Discovery of Peptide and Aptamer Ligands for Targeted Drug Delivery to Hepatic Stellate Cells
… is to present two approaches to identify Hepatic Stellate Cells (HSCs) targeting ligands using phage-display library biopanning and systematic evolution of ligands by exponential enrichment (SELEX). These HSC-specific ligands can be used for diagnosis and therapy of liver fibrosis. The …
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Detection and characterization of rat hepatic stellate cells in a 3-dimensional, perfused, liver bioreactor
… types, and involve mediators presented to the cells in concentrations much higher than physiological values. In addition, the liver lobule contains a 3-dimensional metabolic zonation, and liver cell types comprise a heterogeneous population from the portal triad where the blood flows into the …
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Unfolded Protein Response (UPR) Signaling in Hepatic Stellate Cells (HSC) as a Key Regulator of Liver Fibrosis
… liver disease to cirrhosis is the activation of hepatic stellate cells (HSCs), which secrete extracellular matrix (ECM) proteins such as collagen to form fibrotic scars. Activated HSCs produce vast amounts of fibrotic proteins that require folding and processing prior to secretion. Increased …
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Silencing of endoglin in rat hepatic stellate cells by RNA interference identifies endoglin as an important component of the transdifferentiation process
… the functional characterization of endoglin in hepatic stellate cells (HSC). This characterization was performed particularly with regard to the impact and the function of endoglin in the transdifferentiation process of HSC into myofibroblast-like cells (MFB). Transdifferentiation of HSC into …
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TGF-β Signaltransduktion in der experimentell induzierten Leberfibrose durch die Gallengangsligatur bei der Ratte
… Profibrogenic TGF-beta signaling in hepatic stellate cells is modulated during transdifferentiation. Strategies to abrogate TGF-beta effects provide promising antifibrotic results, however, in vivo data regarding Smad activation during fibrogenesis are scarce. Methods: Here, liver …
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Expression, Reinigung und therapeutischer Einsatz eines löslichen TGF-[beta]-Typ-II-Rezeptors in der experimentell induzierten Leberfibrose
… responsible for the transdifferentiation of hepatic stellate cells to myofiborblasts, the key-event of liverfibrosis. The protein may intercept tgf-beta before binding the hepatic stellate cells. For amplification of the protein COS-7 cells were infected with the adenoviral construct …
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Role of monocyte subsets in chronic liver injury and liver fibrosis
… of WT animals, which peaked at 24h and 48h. Intrahepatic recruitment of the Gr-1hi monocyte subset was markedly reduced in Ccr2-/- and Ccr2-/-Ccr6-/- mice, when compared to WT animals in the model of acute as well as chronic liver injury. In chronic CCl4-mediated liver injury, detailed analysis …
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Kryokonservierung von hepatischen Sternzellen der Ratte
… work a protocoll for the cryopreservation of rat hepatic stellate cells was developed. Isolated HSC of the rat are used as a model of liver fibrogenesis. Many proceedings are studied on this cells, for example the biotransformation, the gene expression, the effect of pharmaceuticals, different …
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Development of Protein Based PCBP2 siRNA Nanocomplex for Liver Fibrosis Therapy
… the poly (rC) binding protein 2 (PCBP2) gene in hepatic stellate cells (HSCs) leads to the reversal of the accumulated extracellular matrix. We therefore hypothesize that targeted delivery of the PCBP2 siRNA to HSCs could potentially treat liver fibrosis. Cholesterol and IGF2R (insulin growth …
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Innate Interleukin-17 and Interleukin-22 play important roles in viral hepatitis
Intrahepatic cell-derived IL-17 and IL-22 are important for T cell priming and hepatoprotection in viral hepatitis, respectively; however, the source and regulation of these cytokines in the liver microenvironment are not well defined. Here, we present evidence for a significant expansion of IL-17- …
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Mapping Human Liver Development using Single-Cell Transcriptomics to Reveal Pathways Directing Stem Cell Fates
… with non-parenchymal cholangiocytes, endothelial cells, Kupffer cells, and hepatic stellate cells. Disorders affecting hepatocytes are life threatening and increasing in prevalence12, with end-stage treatment relying upon a limited supply of liver donations for transplant. Consequently, the …
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Einfluss von BMP-7 und Endoglin auf den TGF-beta Signalweg bei fibrosierenden Lebererkrankungen
… shown that over expression of endoglin in these cells leads on the one hand to an inhibition of the ALK5/Smad3 pathway and on the other hand to an amplification of the BMP-7/Smad1/5 pathway. To further characterize the interaction between BMP-7 and TGF-b1 within liver fibrogenesis hepatic …
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Molecular genetics of patatin-like phospholipase domain-containing 3 and chronic liver disease
… following hypotheses: 1) PNPLA3 is involved in hepatic very low density lipoprotein secretion 2) the protein acts as a glycerolipid hydrolase and the 148M mutation is a loss of function 3) PNPLA3 has a specific role in retinol metabolism in hepatic stellate cells. We tested the first hypothesis …
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Enteric and hepatic glia-like cell reactivation after astrocyte-directed twinkle helicase loss
… out the twinkle gene from astrocytes, glial cells of the brain, causing spongiotic encephalopathy, astrogliosis and ciliogenesis activation in the mouse brain. This model is created by using a glial fibrillary acid (GFAP) promoter, driving a Cre recombinase that together with LoxP sequences …
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Insights into the role of hepatic stromal cells and the immune checkpoint protein PD-L1 in liver metastases of pancreatic cancer
… is the primary metastatic site, yet how the hepatic microenvironment influences disseminated pancreatic ductal epithelial cells (PDEC) remains unclear. While hepatic stellate cells (HSC) dominate physiological liver contexts, hepatic myofibroblasts (HMF) prevail under inflammation. Despite …
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Unterschiede in der TGF-[beta]-vermittelten Signaltransduktion in aktivierten hepatischen Sternzellen unterhalb der Smad2-Phosphorylierung
… activities involved in phenotypic changes of hepatic stellate cells (HSC) have not been characterized in detail. We investigated TGF-beta1-responsiveness of activated HSC and determined Smad2 as intracellular mediator. Gene expression and reporter gene analysis showed, that TGF-beta1 treatment …
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Mechanistic Elucidation of the Function of Sirtuin 6 in the Regulation of Liver Fibrosis
Hepatic fibrosis is a cellular repair mechanism that is initiated upon prolonged damage to the liver, resulting in an accumulation of excess extracellular matrix. This eventually leads to the formation of scar tissue, which disrupts the hepatic architecture and causes liver dysfunction. Hepatic …
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