Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"hepatic cells"”.
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Characterization of foetal hepatic cells during rat liver development
Die Leber entwickelt sich als Leberknospe aus dem Entoderm des unteren Abschnittes des Vorderdarmes. Die Leberknospe differenziert sich in 2 Teile: In den Leberteil und in den Galleteil. Während der embryonalen Entwicklung differenzieren sich die entodermalen Zellen zu Hepatoblasten, die sich zu …
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Regulation of the Gene Encoding Thrombin-Activable Fibrinolysis Inhibitor in Non-Hepatic Cells
… protein in various vascular and inflammatory cells. Specifically, we describe the expression of CPB2 mRNA in the megakaryocytic cell lines MEG-01 and Dami, the monocytic cell line THP-1, and peripheral blood mononuclear cells. TAFI protein was detected in differentiated Dami and THP-1 cells. …
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Engineering human hepatic organoids for in vitro liver modeling and implantable cell therapies
… to regenerate, but methods to expand primary hepatic cells in vitro while supporting their phenotype and function remain elusive. Organoid culture has successfully grown dozens of otherwise difficult-to-culture primary cells in 3D, generating valuable biological models and offering a new …
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Immunotoxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and Diethylstilbestrol (DES) in the Fetal Mouse Thymus and Liver
… noted primarily in cortical zones where pyknotic cells were increased by either TCDD or DES treatment. Using day 18 thymocyte suspensions and flow cytometry, 7-AAD showed decreases in viable thymocytes from TCDD- or DES-treated fetal mice, and concomitant increases in thymocytes in early …
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Transcriptional regulatory elements for basal expression and effects of phenobarbital and dexamethasone onmRNA levels of rabbit CYP2C genes
… involved in the regulation of general as well as hepatic-specific transcription, 5$\sp\prime$-flanking fragments from CYP2C1 (C1) and CYP2C2 (C2) genes were inserted in front of the firefly luciferase reporter gene and analyzed for promoter activity in HepG2 (hepatic) and COS-1 (non-hepatic) …
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EFFECTS OF THE LIVER MICROENVIRONMENT ON HUMAN LIVER SINUSOIDAL ENDOTHELIAL CELLS DURING IN VITRO CULTURE
Liver Sinusoidal Endothelial Cells (LSECs) are specialized endothelial cells that play an important role in the metabolic and xenobiotic clearance functions of the liver. LSECs are distinguished by their hallmark characteristics of lacking a distinct basement membrane and containing open pores …
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Επίδραση της υπεροξίας στο ήπαρ (στο ηπατικό κύτταρο)
… of normobaric hyperoxy on the structure of the hepatic cell and on SGOT and SGPT values. Protection of the hepatic cell with administration of vitamin E, a substance with noted antioxidantive action, was also studied. Seven (7) groups of wistar laboratory rats consisting of ten (10) animals each …
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A novel bioengineering platform using functionalized self-assembly peptides to enhance CYP3A2 activity in modified rat hepatocyte sandwich cultures
… thickness as well scaffold thickness. Thus, the cells are forced into anaerobic metabolic states that degenerate liver specific functions. Furthermore, cells separated from their extracellular matrix and disconnected from the synergistic interactions between other hepatic cells types further …
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The impact of genetic variation in ABCA1 on cholesterol metabolism, atherosclerosis and diabetes
… the major pathway for cholesterol exit from non-hepatic cells and thereby controls the rate-limiting step in the biogenesis of high density lipoprotein (HDL) particles. In humans,ABCA1 deficiency results in Tangier disease, characterized by low levels of HDL cholesterol, cellular cholesterol …
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Investigating Induced Pluripotent Stem Cells for Tissue Engineering and Hepatotoxicity Applications
Induced pluripotent stem cells (iPSCs) can be differentiated into multiple cell types in the body while maintaining proliferative capabilities. The generation of human iPSC-derived hepatocytes (iPSC-Heps) has resulted in a new source for hepatic cells. The current available options for human …
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Engineering Organoids for Stem Cell Maturation
… functions. However, sourcing these adult liver cells is extremely difficult since they are usually obtained through biopsies and are limited in quantity. Induced pluripotent stem cell (iPSC)-hepatocyte-like cells (iHLCs) hold tremendous potential to be used as a substitute since they can be …
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Mapping Human Liver Development using Single-Cell Transcriptomics to Reveal Pathways Directing Stem Cell Fates
… with non-parenchymal cholangiocytes, endothelial cells, Kupffer cells, and hepatic stellate cells. Disorders affecting hepatocytes are life threatening and increasing in prevalence12, with end-stage treatment relying upon a limited supply of liver donations for transplant. Consequently, the …
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Cell cycle regulation in the liver : differential functions of E-type cyclins E1 and E2 for G1/S-phase transition and endoreplication in mice
… are important regulators for driving quiescent cells into the cell cycle. The aim of the present study was to investigate the relevance of cyclin E1 and E2 for directing quiescent hepatic cells into the cell cycle in vivo. In the partial hepatectomy (PH) model it was shown that cyclin E1-/- …
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Proteomic Analysis of Three Dimensional Organotypic Liver Models
… microenvironment are central for understanding hepatic functions and intercellular communication processes. Bottom-up shotgun proteomic analysis of the hepatic cells can lend insight into such processes. This technique employs liquid chromatography-tandem mass spectrometry (LC-MS/MS) for …
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Multi-Cellular Organotypic Liver Models for the Investigation of Chemical Toxicity and Liver Fibrosis
… and disease. Only a limited number of 3D hepatic models incorporate the major liver cell types, and these cultures primarily focus on the hepatocyte response. We have developed 3D liver models that include all major hepatic cell types and recapitulate the layered architecture of the organ. …
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Role of cytochrome P450 enzymes on alcohol/nicotine-mediated oxidative stress and cytotoxicity in monocytes/astrocytes: Implications for HIV-infected alcohol/tobacco users
… in alcohol metabolism in the liver and extra-hepatic cells in chronic users. As a result of alcohol metabolism, CYP2E1 produces reactive oxygen species (ROS), which in turn inhibits acetaldehyde dehydrogenase leading to accumulation of acetaldehyde, both of which are known to damage DNA, …
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Systems Engineering of Stem Cell Fate
Recent advances in the derivation of functional cells from pluripotent stem cells have raised hope for cell therapy to treat liver ailments. They have enhanced the prospects of developing reliable in vitro models for liver diseases and drug toxicity screening. A differentiation protocol mimicking …
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Characterization of PCSK9-mediated LDLR Degradation in Hepatic and Fibroblast Cells
… were detected in several lines of fibroblast cells despite normal LDLR-dependent cellular uptake of PCSK9. Importantly, a PCSK9 degradation assay showed that 125I-labeled wild-type PCSK9 was internalized and degraded equally in both hepatic and fibroblast cells, indicating dissociation of …