Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"heparin-binding"”.
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A peptide model for the heparin binding site on Antithrombin III
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, 1992
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The role of heparin and heparin-binding growth factors in pre-eclampsia
… study tested the hypothesis that expression of heparin-binding growth factors (HBGFs) in normal placental development was altered in a specific pregnancy disorder preeclampsia. HBGFs bind to heparin, a glycosaminoglycan (GAG) affecting activity. I investigated the role of heparin and HBGFs in …
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Innovative Purification Protocol for Heparin Binding Proteins: Relevance in Biopharmaceutical and Biomedical Applications
<p>Heparin binding (HB) proteins mediates a wide range of important cellular processes, which makes this class of proteins biopharmaceutically important. Engineering HB proteins could bring many advantages, but it necessitates cost effective and efficient purification methodologies compared to the …
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Pyridoxylation of the essential lysines in the heparin-binding site of bovine platelet factor 4.
… of bovine platelet factor 4 essential to heparin-binding activity have been determined. The protein was labelled by pyridoxal 5$\sp\prime$-phosphate and reduced by sodium borohydride. Three moles of lysine residues are labelled per mole of platelet factor 4. This resulting modified protein …
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Cytotoxic Activity of Non-Specific Lipid Transfer Protein (nsLTP1) and Proteomic Evaluation of Heparin-Binding Proteins from Ajwain (Trachyspermum ammi) Seeds
<p>Trachyspermum ammi, commonly known as ajwain, is a member of the Apiaceae family. It is a therapeutic herbal spice with diverse pharmacological properties, used in traditional medicine for various ailments. However, all previous studies were conducted using small molecule extracts, leaving the …
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ANTIMICROBIAL ACTIVITIES OF HISTIDINE-RICH GLYCOPROTEIN AND CATIONIC PEPTIDES
… show that structural motifs connected with heparin-binding may confer antimicrobial activity to a given peptide. Peptides from various heparin-binding endogenous proteins exerted antimicrobial activity against Gram-positive and Gram-negative bacteria and similar results were obtained with …
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Identification of seminal proteins related to fertility of bulls
… conducted to determine the chemical identity of heparin binding proteins in semen that are related to fertility of bulls. The first study describes the isolation and identification of a 31,000 dalton fertility-associated antigen (FAA). FAA was found to have significant primary structure homology …
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Novel Endogenous Antimicrobial Peptides
… glycosaminoglycans like dermatan sulphate and heparin were shown to bind to and inactivate LL-37. Furthermore, we demonstrate that structural characteristics associated with heparin affinity (cationicity and amphipathicity) may confer antimicrobial properties to any given peptide. …
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Role of the interaction of proHB-EGF with heparan sulfate proteoglycans
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) exhibits activity as a juxtacrine, paracrine, and autocrine ligand for the epidermal growth factor receptor (EGFR), and possesses the ability to bind heparan sulfate proteoglycans (HSPGs). The interaction of HB-EGF with HSPGs has …
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Engineering the Structure of the Human Acidic Fibroblast Growth Factor to Enhance its Stability and Cell Proliferation Activity
… to the heavily sulfated glycosaminoglycan, heparin, which eliminates the charge-charge repulsion via electrostatic interactions with the positively charged residues near the c-terminus in the region known as the heparin-binding pocket. However, recently several disadvantages have been …
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MOLECULAR MODELING STUDIES OF HEPARIN AND HEPARIN MIMETICS BINDING TO COAGULATION PROTEINS
<p>Heparin, a glycosaminoglycan (GAG), is a complex biopolymer of varying chain length and consisting of uronic acid and glucosamine residues, which are sulfated at various positions. The interaction of heparin with antithrombin is the basis for anticoagulation therapy. Heparin accelerates the …
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A comparative analysis of cowpox virus (CPV WT) and a deletion mutant lacking the gene encoding the inflammation modulatory protein (CPV IMP)
… classical pathways of complement activation by binding to the proteins C3 and C4, thereby preventing complementmediated opsonisation of virus, antibody-mediated lysis of infected cells and migration of inflammatory cells into the site of infection. VCP also possesses heparin binding sites.
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Cryo-electron microscopy of HPV16 pseudovirions reveal changes in capsid conformation upon furin cleavage
… virus capsid attaches to the cell surface via binding to heparan sulphate proteoglycans (HSPGs). Cleavage of L2 by a host protease, furin, is necessary for infection and is thought to facilitate a conformational change in the virus capsid. Furin cleavage may affect the ability of HPV to bind to …
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Pleiotrophin inhibits melanogenesis in normal human melanocyte
Pleiotrophin (PTN) is a secreted heparin-binding protein that is involved in various biological functions of cell growth and differentiation. Little is known about the effects of PTN on the melanocyte function and skin pigmentation. In this study, we investigated whether PTN would affect …
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Regulatory Immune Responses and Repair Mechanisms in Atherosclerosis
… growth factor, epidermal growth factor, heparin-binding epidermal growth factor) measured in plasma can reflect a fibrous plaque phenotype. In particular, high plasma levels of heparin-binding epidermal growth factor at baseline was associated with a decreased risk for developing a …
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Examination of Microsporidia Spore Adherence and Host Cell Infection <em>In Vitro</em>.
… kDa <em>Encephalitozoon cuniculi</em> host cell binding protein was discovered and identified as ECU01_0820, hereafter known as <em>Encephalitozoon cuniculi</em> microsporidia spore adherence protein (EcMsAP). The gene encoding EcMsAP has multiple heparin-binding motifs and an integrin-binding …
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Optimizing chondrogenic factors and protein delivery methods for cartilage repair
… effects of Dexamethasone (Dex) and HB-IGF- I (heparin-binding insulin-like growth factor-1) on cells encapsulated in the self-assembling peptide RAD and agarose hydrogels. Dex is a synthetic corticosteroid that has been shown to improve cartilage-like tissue production by bone marrow stromal …
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The characterisation of the cell surface of Staphylococcus aureus in the search for new therapeutic targets
… protein processing. We also characterised heparin-binding proteins in S. aureus and interrogated the data in light of our surface proteome studies. This approach enabled us to gain insight into novel binding characteristics used by surface-accessible proteins that could not be predicted …
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Effect of disruption of 3D8 complementarity-determining regions on properties of 3D8 antibody
… antibodies have been utilized to improve antigen-binding affinity and/or stability. However, little is reported about functional consequence by the disruption of whole amino acid sequence in CDRs of an antibody with multiple activities. Here, we dissected influence of each whole CDR-disruption on …
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In vitro and in vivo growth factor delivery to chondrocytes and bone-marrow-derived stromal cells in cartilage and in self-assembling peptide scaffolds
… A fusion protein was created by adding a heparin-binding domain to IGF-1 (HBIGF- 1), converting IGF-1 from a short-acting growth factor to one that can be retained and locally delivered in articular cartilage in vivo. It was shown that HB-IGF-1 is retained in cartilage through binding to …
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