Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"heparan sulphate"”.
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An NMR and molecular modelling study on the solution conformation of Heparan Sulphate: new insights into the relationship between structure and function
… all oligosaccharides and their chemically de-2-O-sulphated derivatives have revealed additional new insights into the structural influence of sulphate groups. Consistent with previous studies, at the monosaccharide level, sulphation was found to influence iduronate conformational behaviour. …
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Characterisation of novel matrix-binding interactions for latent transforming growth factor-β-binding protein-2 (LTBP-2), with emphasis on heparin and heparan sulphate proteoglycans.
… fibulin-4, fibulin-5 and as yet unidentified heparan sulphate proteoglycans (HSPGs), have also been identified as playing important roles in this process. Fibrillins-1, -2, -3 and latent transforming growth factor-β binding protein (LTBP)-1, -2, -3, -4, associated components of …
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Biosynthesis and degradation of proteoglycans in cultured smooth muscle cells
… synthesize proteoglycans that are more highly sulphated than those produced in the absence of ascorbate. The glycosaminoglycan chains associated with the proteoglycans synthesized by rat smooth muscle cells were heparan sulphate, chondroitin sulphate and dermatan sulphate. There was no evidence …
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Circulating proteoglycans and glycosaminoglycans during critical illness
… levels of glycosaminoglycans, and in particular heparan sulphate and hyaluronic acid, main glycosaminoglycans on the vessel wall, were higher in in septic shock patients compared to control patients. Furthermore, the concentration was higher in the patients that did not survive the disease. These …
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Syndecan-4 regulates cell-surface trafficking and biological activity of transglutaminase-2
… heparin, and in a previous study cell-surface heparan sulphate (HS) has been implicated in extracellular TG2 mediated RGD-independent cell adhesion, a non-enzymatic process independent from the α5β1 integrin-binding to the RGD domain on FN (Verderio et al., 2003). Hence HS proteoglycan (HSPG) …
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Effects of bFGF (Basic Fibroblast growth factor) on the Haematopoietic Sequelae that follow transplantation
… 0, 2 and 20 ng/ml bFGF with and without 20 ng/ml heparan sulphate (HS). The second aliquot was for the selection of the progenitor population. Stroma was quantitated by placing culture dishes on a grid containing 1mm squares and scoring the number of squares occupied by stromal layers as the …
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Synaptic morphology, function, and regulation in a paediatric-onset neurodegenerative disorder
… sulphamidase gene, leading to accumulation of heparan sulphate. Progressive neurological symptoms are the primary manifestation, including impaired control of motor function. Despite the abnormal intracellular pathology, negligible neuronal cell loss occurs. This suggests that neurological …
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Isolation and characterisation of novel glycosaminoglycan-like polysaccharides derived from marine molluscs with antiproliferative activity
… chains; therefore, a new type of marine-derived heparan sulphate/heparin-like polysaccharide with potent anticancer properties was suggested, as none of the mammalian glycosaminoglycans exhibited antiproliferative activity on cancer cell lines. Overall, the final structural characterisation did …
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On the Role of Polyamines and Microvesicles in Tumour Development. Regulation by Hypoxia and Implications for Therapeutic Intervention of Cancer.
… epitope-specific interference of cell surface heparan sulphate proteoglycans (HSPGs) by anti-HS antibodies inhibits the bioavailability of exogenous polyamines, and that combined targeting of polyamine biosynthesis by α-difluoromethylornithine (DFMO) and HSPG-dependent uptake by a HIV-1 …
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Antibody production with synthetic peptides against human Coronavirus-NL63 ORF3 protein
… simplifies binding to ACE2 by attaching to heparan sulphate proteoglycans on the cell surface. ACE2 has a significant expression in airway differentiated epithelial cells and CoV‐NL63 enter these cells from their apical surface. It is imperative to note that CoV‐NL63, CoV‐229E, CoVHKU1 and …
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Molecular insights into signalling mechanisms within TGF-β superfamily
… domain hindering follistain’s ability to bind to heparan sulphate and to inhibit TGF-β superfamily signalling molecules. Additionally, SAXS-based modelling of follistatin’s solution structure demonstrates that all three full-length follistatin isoforms alternate between two different solution …
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The Mechanisms of Tau Entry to the Cytosol
… tau assemblies, surface receptor LRP1 and heparan sulphate proteoglycans promote the uptake of tau assemblies to membrane-bound vesicles. A subsequent escape from these vesicles is postulated for assemblies to enter the cytosol and contact cytosolic tau pools to induce seeded aggregation. …
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Cryo-electron microscopy of HPV16 pseudovirions reveal changes in capsid conformation upon furin cleavage
… attaches to the cell surface via binding to heparan sulphate proteoglycans (HSPGs). Cleavage of L2 by a host protease, furin, is necessary for infection and is thought to facilitate a conformational change in the virus capsid. Furin cleavage may affect the ability of HPV to bind to sulphated …
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Reiterative FGF signaling determines the identity and morphology of the lacrimal gland
… which binds to epithelial Fgfr2b and co-receptor heparan sulphate proteoglycans, to promote budding and branching morphogenesis of the lacrimal gland. However, the mechanism by which Fgf10 expression is regulated within the neural crest and the direct downstream targets of Fgf signaling in the …
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Cell surface interactions of Coxsackie A9 virus
… CAV9 has also been reported to interact with the heparan sulphate/heparin class of proteoglycans (HSPG). This thesis describes work designed to improve our understanding of the involvement of a) the RGD motif and more specifically the RGDX position in CAV9 infection, using a large panel of …
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Characterisation of the pro-regenerative extracellular matrix in the zebrafish spinal cord
… Tests using heparin-derived mimetics (mHeps) of heparan sulphate proteoglycans (HSPGs) indicate that a densely-sulphated glycan structure shown to be anti-regenerative in MC-Inj (mHep1) stimulates axonal regeneration in the zebrafish. A screen of specifically modified variations on this structure …
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Sequence-structure-function relationships of glycosyltransferases in families GT43, GT47, and GT64
… glycosyltransferases synthesise the backbone of heparan sulphate, and contain both a GT47 and a GT64 domain. Using cryo-EM, I solved the structure of EXTL3, the largest exostosin, in complex with UDP. The structure revealed that the EXTL3 GT47 domain adopts a GT-B fold that has been inactivated …
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Characterisation of human surfactant protein A and recombinant human vimentin in their modulation of HPV16 pseudovirus infection
… to a lesser degree, with surface-expressed heparan sulphate proteoglycans (HSPGs) than control particles. Removal of surface HSPGs on NIKS cells decreased HPV16-PsVs cell surface binding and internalisation, while pre-incubation of HPV16-PsVs with rhVim decreased viral particle binding and …
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Alpha-L-iduronidase transduced mesenchymal stem cells as a therapy for the treatment of CNS degeneration in mucopolysaccharidosis type I mice
… resulting in the accumulation of undegraded heparan sulphate and dermatan sulphate glycosaminoglycans (gags) within the lysosome of nearly every cell. MPS I is a multi-tissue and organ disease, presenting with profound mental retardation and skeletal abnormalities. Haematopoietic stem cell …
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Immune responses to AAV gene therapy in the ocular compartment
… binding affinity to the AAV2 primary receptor, heparan sulphate proteoglycan, and as such it was suggested that reduced sequestration of virions in the heparan-rich inner limiting membrane may increase cellular infection levels in the retina, thereby increasing overall immune activation. The …
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