Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"heparan sulfate proteoglycans"”.
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Heparan Sulfate Proteoglycans in Pancreatic Ductal Adenocarcinoma (PDAC)
<p>We have previously identified a heparan sulfate proteoglycan (HSPG), Syndecan-1 (SDC1), as a key effector for pancreatic ductal adenocarcinoma (PDAC) progression whose surface expression is driven by KRAS*. By leveraging various PDAC models, we found that surface SDC1 expression was initially …
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Role of the interaction of proHB-EGF with heparan sulfate proteoglycans
… (EGFR), and possesses the ability to bind heparan sulfate proteoglycans (HSPGs). The interaction of HB-EGF with HSPGs has been previously studied only with the soluble (autocrine/paracrine) form of the protein (sHB-EGF), produced after proteolytic cleavage of the transmembrane form …
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Defining the role of heparan sulfate proteoglycans in APL-1 function in Caenorhabditis elegans
… to guide sAPL-1 to its target receptors. Heparin sulfate proteoglycans (HSPGs), such as LON-2 glypican and UNC-52 perlecan, are likely candidates for the ECM proteins that interact and guide sAPL-1. The disruption of APL-1 causes phenotypical changes, including in learning, reproduction, and …
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The role of heparan sulfate proteoglycans and heparanase in the control of vascular remodeling
… atherosclerosis and restenosis. We examined heparan sulfate proteoglycan homeostasis as a mechanism of regulation of arterial vascular remodeling in response to altered mechanical environments such as hypertension and injury. We first studied the effect of in-vitro mechanical strain on the …
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Characterization of Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) interaction with the Bovine Aortic Endothelial (BAE) cell surface: Examination of the Role of Heparan Sulfate Proteoglycans (HSPG)
… value of 3.1 x 10<sup>-10</sup> M. The role of heparan sulfate proteoglycans (HSPG) in the IGFBP-3 binding mechanism was also examined. It was seen that inactivation of the cell surface HSPGs with 75 mM sodium chlorate did not affect IGFBP-3 binding. Further, there have been reports of …
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Mutational Analysis of the Export Targeting Motif of Fibroblast Growth Factor 2, a Mediator of Tumor-Induced Angiogenesis
… since exported FGF2-GFP binds to cell surface heparan sulfate proteoglycans and, therefore, is accessible by membrane-impermeable tools such as antibodies and biotinylation reagents. In the second part of this thesis, a systematic mutational analysis of the FGF2 open reading frame was conducted …
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Uterine Remodeling During Pregnancy - Studies on the Effect of Heparin/Heparan Sulfate
… of the uterine cervix preceding labor. Moreover, heparan sulfate proteoglycans appear to be central for the remodeling of the cervix and uterus before parturition, as shown by the finding that heparin fragments can be used in a clinical setting to prevent protracted labor.
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Identification of the receptor for cellular internalization of an anti-nucleic acid antibody, 3D8 scFv
… 3D8 scFv bound to both cell surface heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans (CSPGs) that are highly negatively charged molecules known as endocytic receptors for a variety of ligands. Treatment of soluble HS or CS reduced cell surface binding of 3D8 …
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Roles of Heparan sulfate in mesendoderm differentiation of human embryonic stem cells
… and extrinsic signals in the stem cell niche. Heparan sulfate proteoglycans (HSPGs) are found on the membrane of all animal cells and have long been implicated in a wide range of cell-cell signaling and cell-matrix interactions. Multiple heparan sulfate (HS)-binding growth factors, such as Wnt, …
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Modeling of Therapeutic Drugs, Polymers, and Self-Assembling Peptides
… of different topologies and sizes that can mimic heparan sulfate proteoglycans. We also investigated cucurbit[n]urils and small drugs capable of encapsulating charged viral residues and performing other inhibitory activities. B) First, we developed methods capable of designing small therapeutic …
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The Regulation and Mechanism of Myelin Synthesis Involving Endogenous Steroid Hormones
… basic carboxy-terminal to membrane associated heparan sulfate proteoglycans (HSPG), surface competition experiments were performed with heparin and the basic peptide encoded by exon 6. Heparin and the exon 6 peptide were both found to dramatically inhibit the initiation of myelin formation. …
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Endocytosis of therapeutic macromolecules in tumor cells - Mechanistic aspects of the proteoglycan receptor function
… delivery methods depend on cell surface heparan sulfate proteoglycans (HS PGs) for efficient uptake, however the details of this mechanism and the exact role of the PG has been unclear. The aim of this thesis was to clarify the role of the cell surface PG in macromolecular uptake …
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Molecular Basis of Adaptation of Enteroviruses to Different Cancer Cell Lines
… αvβ6. Some isolates of CVA9 also bind to heparan sulfate proteoglycans (HSPG). This thesis describes work designed to improve our understanding of CVA9 and echovirus cell/receptor tropism. Several echoviruses and CVA9 variants were tested in a panel of 9 cell lines. Distinct patterns of …
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The Heparan Sulfate Proteoglycan Perlecan Regulates Axonal and Synaptic Stability
Heparan sulfate proteoglycans (HSPGs) form essential components of the extracellular matrix (ECM) and basement membrane (BM) and have both structural and signaling roles. Perlecan is a secreted ECM-localized HSPG that contributes to tissue integrity and cell-cell communication. In this thesis, I …
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Expression and Transcriptional Regulation of APL-1, a <i>Caenorhabditis elegans</i> APP Ortholog
… that extracellular matrix proteins, particularly heparan sulfate proteoglycans, are involved in shuttling the extracellular domain sAPL-1 to activate its targets and also towards degradation in the coelomocytes. Finally, we identify genetic factors that control APL-1 expression, and putative …
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Dynamic visualization and genetic determinants of Sonic hedgehog protein distribution during zebrafish embryonic development
… required for the synthesis of Heparan Sulfate Proteoglycans and established a tentative link of these genes to somitic Hh signaling. In addition, I characterized the developmental expression and function of zebrafish Rab23, a small GTPase, which acts as a negative regulator of …
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Mechanistic studies on the role of extracellular vesicles in the tumor microenvironment
… the glycan chains of the cell surface receptors, heparan sulfate proteoglycans (PGs). We further show that EVs are internalized through a lipid raft-mediated endocytosis pathway negatively regulated by caveolin-1. In the second part (papers III and IV), we show that EVs originating from hypoxic …
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Analysis of Cellular Factors Involved in Adeno-Associated Virus Type 2 Entry
… of sugar modified membrane protein known as heparan sulfate proteoglycans, have been shown to be a critical determinant in the efficiency of infection of many important pathogens. Adeno-associated virus type 2 (AAV) is a small (25nm) single stranded DNA, non-enveloped parvovirus that is …
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The Role of Stromal-Derived Factors in Neuroblastoma Differentiation
… differentiation and decreased proliferation. Heparan sulfate proteoglycans (HSPGs) and heparin derivatives further enhance HBEGF-induced differentiation by forming a complex with the epidermal growth factor receptor (EGFR), leading to activation of the ERK1/2 and STAT3 pathways and …
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