Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"hematopoietic progenitor cells"”.
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Retinoic Acid Pathway Inhibition to Expand Human Hematopoietic Progenitor Cells with Islet Regenerative Capacity
… for diabetes. Umbilical cord blood (UCB)-derived hematopoietic stem/progenitor cells (HSPC) isolated by high aldehyde dehydrogenase activity (ALDHhi) reduce hyperglycemia after transplantation into streptozotocin (STZ)-treated NOD/SCID mice. However, UCB-derived ALDHhi cells are rare and expansion …
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Characterization of Genome-Wide Binding of NUCLEAR-FACTOR I-X in Hematopoietic Progenitor Cells
… of c-Mpl transcription can protect primitive hematopoietic cells from stress ex vivo. Understanding the direct transcriptional targets or co-binding partners of NFIX would provide further insight into the mechanisms HSPC employ to maintain steady-state hematopoiesis or overcome stress …
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Identification of the Downstream Effector Genes Involved in HOXB4-Induced Expansion of Hematopoietic Progenitor Cells
… factor family, promotes expansion of hematopoietic stem and progenitor cells both<em>in vivo </em>and <em>in vitro</em>.<em> </em>However, the molecular mechanisms underlying this effect are not well understood. In order to identify direct target genes of HOXB4 in primary murine …
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The Wilms' tumor gene 1 (WT1) and leukemia -new insights and further complexity
… factor which is highly expressed in immature hematopoietic progenitor cells. A high expression of WT1 and the presence of somatic mutations in acute leukemia indicate a role for WT1 in the pathogenesis of leukemia. The ojective of this thesis was to investigate the role of WT1 during human …
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The Role of AF9 and AF9-Mediated Protein Interactions in Hematopoiesis and Leukemogenesis
… leukemic transformation.</p><p>Using bone marrow progenitor cells isolated from conditional Af9 knockout mice, we examined in vitro differentiation of hematopoietic progenitor cells to the erythroid, myeloid and megakaryocytic lineages in the presence or absence of Af9. Based on previously …
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Investigation of a suppression of asymmetric cell kinetics (SACK) approach for ex vivo expansion of human hematopoietic stem cells
Ex vivo expansion of hematopoietic stem cells (HSCs) is a long-standing challenge faced by both researchers and clinicians. To date, no robust, efficient method for the pure, ex vivo expansion of human HSCs has been demonstrated. Previous methods primarily induced the expansion of committed …
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A novel principle for immunomodulator delivery with hematopoietic cells as vehicles Focus on soluble TNF-receptor gene expression, targeting to secretory lysosomes and regulated secretion
The goal of this research is to use hematopoietic cells as vehicles for targeting transgenic immunomodulators to the inflammatory sites. The work presented was focused on the anti-inflammatory soluble TNF receptor. A transmembrane soluble TNF receptor (sTNFR1) construct was expressed in …
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Effect of CDDO-me on Myelopoiesis in Naive Mice and Mice Undergoing Bone Marrow Transplantation (BMT)
… other hematological diseases, large numbers of hematopoietic stem cells (HSC) and hematopoietic progenitor cells (HPC) are needed. The objectives of this study were to examine the toxicity effect of the synthetic triterpenoid CDDO-me in vivo, to investigate the effect of CDDO-me on myeloid …
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Studies on signaling pathways induced by FLT3, an important oncogene in AML
FLT3, a receptor tyrosine kinase, is expressed in hematopoietic progenitor cells. FLT3-ITD (internal tandem duplication) and D835 mutations are found in approximately 30% and 7% of Acute Myeloid Leukemia (AML) patients respectively, and correlate with a poor prognosis, thus making the mutated …
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Upstream Migration Under Shear Flow: Force Generation and Molecular Regulation
… a mechanically anomalous behavior implicated in hematopoietic stem cell trafficking and immune cell surveillance. Despite the centrality of mechanical force to this process, the forces upstream-migrating cells generate at the cell-substrate interface have not been directly measured during …
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Functions of Novel Ligand-independent Flt3 Alleles and RANKL in Promoting Dissemination of Murine B-Cell Leukemias to the Central Nervous System
… that drive CNS dissemination of leukemic B-cells. We show that CNS-disseminating B-ALLs had recurrent genomic rearrangements that replaced N-terminal Fms-like tyrosine kinase 3 (Flt3) exons with endogenous retrovirus (ERV) transcriptional control elements. ERV-Flt3 fusion genes encoded …
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The Role of Hspa9 in Mouse Hematopoiesis and IL-7 Receptor Signaling
… with myelodysplastic syndrome (MDS), a clonal hematopoietic stem cell disorder. Cytogenetic abnormalities occur in ~50% of MDS patients and an interstitial deletion or loss of chromosome 5 containing HSPA9 is the most common, occurring in up to 25% of patients. In order to understand the role …
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Signaling pathways regulating self-renewal, differentiation, and multipotency of CD133+ umbilical cord blood stem cells
… the number of immature umbilical cord blood stem cells (UCBSCs) for hematopoietic transplantation. Towards this goal, our lab adapted a culture technique to grow immature CD133+ UCBSCs (CD133+ cells). Although CD133+ cells proliferate rapidly in culture, a minority self-renew and remain CD133+, …
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THE ROLE OF SDF-1[alpha] AS A VASCULOGENIC CHEMOKINE AND ENDOTHELIUM-ASSOCIATED CELL ADHESION MOLECULE FOR THE RECRUITMENT OF BONE MARROW-DERIVED PROGENITOR CELLS TO DEVELOPING TUMORS
… or CXCL12) is a chemoattractant for lymphocytes, hematopoietic progenitor cells, and vascular endothelial cells. SDF-1 alpha binds to the CXCR4 receptor on cell surfaces and to heparan sulfate in cell membranes and the extracellular matrix. We hypothesized that SDF-1 alpha is an important mediator …
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Understanding and Exploiting Viral Protein US28 During Human Cytomegalovirus Latency
… site of HCMV latency is early myeloid lineage cells, including hematopoietic progenitor cells and monocytes, in which the critical viral lytic promoter, the major immediate early promoter (MIEP), is repressed. This is mediated by a combination of host and viral factors, including the viral …
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WILMS’ TUMOUR GENE 1 PROTEIN (WT1) – AN EFFECTOR IN LEUKEMOGENESIS?
… WT1 is expressed in a small subset of early progenitor cells, with declining levels as maturation occurs into mature blood cells. However, most primary leukemias express high levels of WT1 at diagnosis and this is an independent predictor of adverse outcome. In paper I, I demonstrate that WT1 …
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Characterization of Thy-1 expression on human hematopoietic cells
Hematopoietic stem cells have extensive proliferative capacity and the ability to produce daughter cells of all hematopoietic lineages. However, their characterization is complicated by their low frequency and the heterogeneous cellular composition of the tissues in which they are found. The …
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Natural and Exogenous Genome Editing In Wiskott-Aldrich Syndrome Patient Cells
… the WAS protein (WASp), exclusively expressed in hematopoietic cells and required for proper platelet production and lymphoid cell function. Approximately 11% of patients with WAS exhibit a phenomenon called Somatic Revertant Mosaicism which is characterized by the presence of lymphocytes which …
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Oncogenic roles of RAS in acute myeloid leukemia cooperated with Mll-AF9.
… in this thesis. First, we investigated which hematopoietic cells express TRE -driven transgenes when combined with Vav-tTA , because mastocytosis was developed in Vav-tTA ; TRE-NRAS G12V transgenic mice without detectable other diseases. We assayed Vav-tTA -driven luciferase expression in …
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The Role of Meis1 in Hematopoietic Development
… cell fate in differentiating embryonic stem cells: ESCs). To understand the molecular mechanisms underlying the actions of <italic>Mesp1</italic>, we sought to identify transcription targets of <italic>Mesp1</italic>. <italic>Mesp1</italic> rapidly induced expression of PDGFRα in …
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