Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 5 of 5 for “"helper phage"”.
-
Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins.
… and are mobilized at high frequency by specific helper bacteriophages. One remarkable feature of helper phage exploitation by SaPIs is remodeling of the normal T=7 bacteriophage capsid to produce smaller T=4 phage-like particles. These particles, composed entirely of helper phage proteins, can …
-
Identification and Characterization of Helper Phage Gene Products Involved in Mobilization of Staphylococcal Pathogenicity Island SaPI1
… during the vegetative growth of staphylococcal phage 80α. The amplified genetic element is subsequently encapsidated and transduced at very high frequency. Previous studies have demonstrated that the transducing particles have virions with tails that appear identical to those of helper phage 80α …
-
Single-Stranded-Dna Sp6 and T7 Bacteriophage Promoter Plasmids for Engineering Mutant Rnas and Proteins: Extensions and Deletions at the Amino Terminus Which Alter the Activity of Preproparathyroid Hormone Signal Peptide
… proteins, all from a single vector. Chimeric phage-plasmid expression vectors, or phagemids, containing the intergenic region of the single stranded DNA bacteriophage f1 and either a bacteriophage SP6 or T7 promoter have been constructed. Coinfection of E. coli with these chimeric plasmids and …
-
Role of the Small Terminase Subunit Encoded by Staphylococcus Aureus Pathogenicity Island SaPI1 in Formation of SaPI1 Transducing Particles
… is mobilized and transduced at high frequency by helper phage 80α. SaPI1 encodes a small terminase protein that belongs to the phage small terminase subunit family. The presence of SaPI1-encoded small terminase suggests that it plays a role in SaPI1-specific packaging into transducing particles by …
-
Analysis, Optimization and Application of AAV Capsid Assembly using Escherichia coli
… VP3 capsid protein. AAV ssDNA was produced using phagemids and M13 helper phage. The produced circular ssDNA with an expression cassette flanked by AAV inverted terminal repeat sequences (ITRs) mediated protein expression in CHO-K1 cells. The ARM_VP3 fusion protein was successfully obtained from …