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Showing 1 to 20 of 24 for “"helix-turn-helix"”.
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I. Collagen-like polypeptides. II. Helix-turn-helix peptides and turn mimetics
… mammals. It consists of three left-handed PPII helixes coiled along a common axis to form a very compact right-handed super helix. The primary structure is shown to be (Gly-Xaa-Yaa)n repeats with high content of prolyl residues at both Xaa and Yaa positions. <i>Cis-trans</i> isomerization of the …
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Part 1 Synthesis of a potent histone deacetylase inhibitor; Part 2 Studies towards a stabilized helix-turn-helix peptide
… in the design of HDIs. The hydrophobic cyclic turn mimic allows the formation of a tight complex between HDI and HDAC enzymes. The second part of this work is to synthesize secondary structure mimics and incorporate them into the helix-turn-helix (HTH) motif. One of the important methods to …
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Structure and Function of Bordetella Transcription Factor BpsR
… as well as analyze residues within the (winged) Helix-turn-Helix DNA binding domain likely to confer BpsR affinity for the bpsA promoter. Sequence alignment of BpsR-like proteins revealed a novel CxxxxxxxC motif in BpsR that contributes to regulation of DNA binding. Additionally, it was found …
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Structural Basis for Signal Transduction in LOV Blue Light Photoreceptors
… LOV domain and a C-terminal DNA binding helix-turn-helix (HTH) domain. Following initial characterization of this protein, I was able to determine how light-induced covalent adduct formation in the N-terminus leads to interdomain separation through release of inhibitory contacts with the …
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Genetic interaction landscape of the Escherichia coli transcriptional factor machinery
… of sensory ligand-binding and DNA-binding helix-turn-helix (HTH) domains to respond to the environmental and internal stimuli. Although Escherichia coli is a well-studied model bacterium, yet half of its TFs’ characteristics remain unclear, including 72 genes of unknown function. Using E. …
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Function and Environmental Regulation ofkpsF, the First Gene of the Region One Operon of Escherichia Coli K1
… (37$\sp\circ$C or 98.6$\sp\circ$F), but is turned off at temperatures commonly encountered in the external environment, below about 26$\sp\circ$C. Expression of the first gene of the region 1 operon, kpsF, is controlled by thermal regulation of a $\sigma7$0-type promoter. Thus, …
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Molecular Mechanisms of DNA Protection by Tardigrade Damage Suppressor Protein (Dsup): Structural Adaptation and Functional Interactions
… and C-terminal regions and a central amphipathic helix forming a helix-turn-helix motif and highly charged C-terminal domain. The C-terminal domain, enriched in positively charged residues, mediates strong electrostatic interactions with DNA, while the helical region stabilizes the complex through …
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The regulation and two genes in Bacteroides thetaiotaomicron starch utilization system
… amino acid sequence of SusR protein had a helix-turn-helix motif at its carboxyterminal end and this region had highest sequence similarity to the corresponding regions of known transcriptional activators. A disruption in susR eliminated expression of all known sus structural genes, as …
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Effect of Autoregulated TxeR on the Expression of <I>Clostridium difficile</I> Toxins
… txeR predicts a 22-kDa protein that contains a helix-turn-helix motif characteristic of DNA binding regulatory proteins. To determine if the protein TxeR regulates expression from the toxA, toxB, and txeR promoters, gene fusions were constructed that contained the various promoter regions and a …
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The Functional Characterization of the LysR-Type Transcriptional Regulator QseD and the SorC-Type Transcriptional Regulator LsrR in Enterohemorrhagic Escherichia coli
… contain two functional domains, an N-terminal helix-turn-helix (HTH) and a C-terminal co-factor binding domain which allows for oligomerization [5]. We have demonstrated that QseD indirectly represses transcription of the LEE in EHEC and represses the flagella regulon expression in K-12 E. …
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The Structural and Functional Analysis of a Voltage-Dependent Potassium Channel
… contain "voltage-sensor paddles"— hydrophobic helix-turn-helix structures located on the channel's outer perimeter. The gating charge residues are embedded in a helical segment of each voltage-sensor paddle. The crystal structures suggest that the voltage-sensor paddles are attached to the …
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Stability, folding and evolution of the tumour suppressor protein p16.
… sequence motif that adopts a β-hairpin-helix-turn-helix fold. Multiple repeats stack in a linear manner to produce an elongated structure that is stabilized predominantly by short-range interactions between residues. Purely composed of four ANK repeats, p16 is the minimal ANK-fold …
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Structure and function of the repressor and operators of the sn-glycerol-3-phosphate regulon of Escherichia coli K-12
… of the purified gip repressor. The presumptive helix-turn-helix region of the repressor was located near the N-terminus (amino acids 22 to 41) at a poSition analogous to that found for the operator binding domain of other repressors such as the deo and Jac repressors. The recognition helix of …
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Dynamic Regulation of Metabolism in Archaea
… in archaea. Previous work identified the winged helix-turn-helix transcription factor TrmB as a major regulator of metabolism in the model archaeon Halobacterium salinarum. TrmB was found to bind to the promoter of 113 genes in the absence of glucose. Many of these genes encode enzymes involved …
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Strengths and Weaknesses of Hybrid TPR Technology for Obtaining Structural and Mechanistic Insights into TPR Proteins
… (TPR) repeats are a 34-residue helix-turn-helix motif that when repeated pack into a superhelical structure. TPR domains are frequently found mediating protein-protein interactions, often through a central groove. One protein complex bearing numerous TPR repeats is the Anaphase …
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Protein/DNA interactions during site-specific recombination
… for detectable excision. Orf2x has a putative helix-turn-helix motif and interacts specifically with the excisive attachment site attL. I purified IntN1 and partially purified Orf2x, then performed DNase I footprinting experiments with fluorescently-labeled DNA containing the NBU1 attachment …
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DNA-Bindung des Transkriptionsfaktors ABF1 aus S. cerevisiae: Spezifität und Kontaktpunkte
… Domänen von ABF1 (Zink-Finger-Motiv, Helix-Turn-Helix-Motiv (HTH) und C-terminale Transaktivierungsdomäne). Mittels Gelretardationsexperimenten mit verschiedenen DNA-Sonden wurde gezeigt, dass ABF1 einen richtungsorientierten DNA-Bindungsmechanismus aufweist und eine Neigung zur …
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Functional Characterization of Atxa, The Bacillus Anthracis Virulence Regulator
… region of the AtxA protein contains winged-helix (WH) and helix-turn-helix (HTH) motifs, structural features associated with DNA-binding. Using filter binding assays, I determined that AtxA interacted non-specifically at a low nanomolar affinity with a target promoter (P<em>lef</em>) and …
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Mechanistic Studies of the Roles of the Transcriptional Activator ExsA and Anti-activator Protein ExsD in the Regulation of the Type Three Secretion System in Pseudomonas aeruginosa
… family are characterized by the presence of two helix-turn-helix (HTH) motifs, which bind to the promoter DNA and activate transcription. ExsA uses its HTH containing C-terminal domain (CTD) to regulate gene expression from 10 different promoters. The N-terminal domain (NTD) of ExsA mediates …
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Transcriptional regulation and enzymatic functions of the Escherichia coli biotin protein ligase
… protein ligase of E. coli belongs to the winged helix-turn-helix (wHTH) family of transcriptional regulators. The N-terminal BirA domain is required for both transcriptional regulation of biotin synthesis and biotin protein ligase activity. In Chapter 3, I addressed the structural and functional …
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