Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"helicase unwinding"”.
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Single molecule studies of helicase mechanisms
Bloom helicase (BLM) is a member of the RecQ helicase family, a group of 3’ to 5’ helicases which are homologs of the RecQ protein found in E. coli. Besides the canonical helicase function of unwinding duplex DNA, the RecQ family of helicases is known to preferentially bind to non-canonical DNA …
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Single molecule fluorescence studies of replicative helicases
DNA helicases are motor enzymes that convert the chemical energy of nucleotide triphosphate hydrolysis into mechanical energy for translocation on single stranded (ss) DNA and unwinding of double stranded (ds) DNA. All of the 12 known replicative helicases are hexameric helicases that are …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
… often averaged out and lost in ensemble assays. Helicases form a ubiquitous class of enzymes that function in many aspects of nucleic acid metabolism which is central to life. We used single molecule Förster resonance energy transfer (FRET) and optical tweezers force spectroscopy to study …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
… often averaged out and lost in ensemble assays. Helicases form a ubiquitous class of enzymes that function in many aspects of nucleic acid metabolism which is central to life. We used single molecule Förster resonance energy transfer (FRET) and optical tweezers force spectroscopy to study …