Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"haptens"”.
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Novel antibody-based biosensors for the detection of haptens, proteins and whole cells
… improved biosensors for the detection of small haptens, proteins and whole cells. These Included fluorescence, chemiluminescence and impedance-based detection systems. Microtitre plates, polymer chps, fluorescence-enhancement chips and screen-printed electrodes were all examined. The specific …
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Molecular mechanisms and complete antigen formation in allergic contact dermatitis
… disease. Small reactive organic molecules called haptens induce ACD. Haptens are not recognized by themselves, but need to bond to endogenous proteins in the skin, and processed further into complete antigens. Antigen specific T cells then recognize the resulting antigen. Although the haptens are …
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Catalytic antibodies for amide cleavage and prodrug activation
… to generate amide cleaving antibodies. Two haptens, O1 and O2, were designed and synthesized to create a hydrophobic pocket for an auxiliary nucleophile as well as elicit complementary basic/acidic side chain residues in the antibody combining binding site. The recently established …
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Detection of colorectal carcinoma-associated antigens using specific antibodies against human milk oligosaccharides
… were used to detect their homologous haptens as gangliosides in the human colorectal carcinoma cell line SW'lll6. Sialyltetrasaccharide b-ceramide was detected in the monosialylganglioside fraction from human meconium and a total ganglioside fraction from SWlll6 cells on thin layer …
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Herstellung von katalytischen monoklonalen Antikörpern
… have only a limited applicability for human use. Haptens and substrates have been synthesized with predefined entantiomeric key structures representing the chroman ring system of vitamin E. Mice strains with a higher property of forming catalytic antibodies were immunized with KLH conjugated …
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Einfluss der Osteopontin Defizienz bei Mäusen auf allergische Hautreaktionen vom Spättyp
… zu Wildtyp Mäusen. Bei Verwendung eines anderen Haptens (TNCB) wurde diese Veränderung der zellulären Zusammensetzung der regionalen Lymphknoten nicht beobachtet. Auch im Rahmen von Restimulationsversuchen konnte im TNCB-Modell kein Unterschied bezüglich Proliferation, Zytokinproduktion und …
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Molekulare Grundlagen der T-Zell Aktivierung durch Haptene : Analyse eines murinen T-Zell Klons mit zwei Antigenrezeptoren
… die molekularen Grundlagen der Erkennung des Haptens TNP (Trinitrophenyl) am Beispiel des Klasse-I MHC (H2-Kb) restringierten murinen T-Zell Klons E6. Als Angehöriger einer ca. 10 igen Minderheit von T-Zellen exprimiert Klon E6 neben einer TCR b-Kette (Vb16) zwei funktionelle a-Ketten (Va3 und …
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METABOLIC AND PHARMACOKINETIC STUDIES OF BENZTROPINE AND TRIHEXYPHENIDYL
… hydroxylated trihexyphenidyl in human urine. Two haptens, benztropine hemisuccinate and trihexyphenidyl hemisuccinate, were synthesized for the development of RIA procedures for benztropine and trihexyphenidyl, respectively. The haptens were coupled to bovine serum albumin or porcine thyroglobulin …
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Entwicklung eines Biosensors zum Nachweis von Antibiotika und Sulfonamiden in Milch- Herstellung der immunchemischen Komponenten
… of coating-antigens was prepared by linking haptens to carrier-proteins. After optimizing test sensitivity and characterizing the test specificity indirect EIAs could be developed for each antimicrobial compound, fulfilling the MRL requirements due to EU regulation 2377/90. Only for …
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Immunoassays with chemically modified bacteriophage
… extended to antibodies against a wide variety of haptens and proteins. Phage particles that have been modified by attaching some molecule onto their surface are sensitive to antibodies directed against the coupled chemical moiety. Furthermore, the inactivation of the modified phage by antibody can …
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Nanoscale antigen organization regulates binding to specific B-cells and B-cell activation
… structural requirements originally defined for haptens triggering T-cell-independent stimulation of B cells, we investigated how the mode of antigen presentation, antigen density, particle size, and lipid mobility influence B cell receptor (BCR) crosslinking by multivalent antigen-bearing …
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Versuche zur Gewinnung von katalytischen Antikörpern zur Hydrolyse von Arylcarbamaten und Arylharnstoffen
Im Rahmen dieser Arbeit gelang es, katalytische Antikörper zur Hydrolyse von Benzylphenylcarbamaten sowie zahlreiche monoklonale Antikörper gegen Haptene herzustellen. Es wurden verschiedene Hapten-Protein-Konjugate unter Verwendung unterschiedlicher Kopplungsmethoden hergestellt und …