Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"hanging drop"”.
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Controlling the Physico-Chemical Microenvironment for Low-Density Neuronal Analysis in Cultures and Microfluidics
… beginning with the first cultures in the hanging drop by Ross G. Harrison to the modern methods of microislands, Campenot chambers, patterned substrates and microfluidics.
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Generating hair follicle inductive dermal papillae cells from adipose derived mesenchymal stem cells
… hair follicle DP-like fate by culture using the hanging drop method and exposure to Wnt, mimicking signalling and mesenchymal condensation in embryological hair follicle induction. Gene expression analysis using RT-PCR showed that the DP-cell marker Versican is expressed at high levels in ASCs …
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ΗΛΕΚΤΡΟΔΙΕΠΙΦΑΝΕΙΑΚΗ ΣΥΜΠΕΡΙΦΟΡΑ ΤΕΤΡΑΥΠΟΚΑΤΕΣΤΗΜΕΝΩΝ ΦΩΣΦΩΝΙΟΚΑΤΙΟΝΤΩΝ ΣΤΗ ΔΙΑΦΑΣΙΚΗ ΠΕΡΙΟΧΗ ΥΔΡΑΡΓΥΡΟΥ/ΔΙΑΛΥΜΑΤΟΣ ΣΕ ΜΕΘΑΝΟΛΙΚΑ ΗΛΕΚΤΡΟΛΥΤΙΚΑ ΣΥΣΤΗΜΑΤΑ
… [PH4]+ CATIONS FROM METHANOLIC SOLUTIONS AT THE DROPPING AND HANGING DROP MERCURY ELECTRODE. THE ADSORPTION PARAMETERS WERE DETERMINED ON THEBASIS OF OUR EXPERIMENTAL DATA AND THE NON IONIC ADSORPTION ISOTHERMS EXCEPT THE MAXIMUM SURFACE CONCENTRATION WHICH WAS DETERMINED FROM CAPACITANCE …
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Effects of Diethylstilbestrol on Murine Early Embryonic Stem Cell Differentiation Using an Embryoid Body Culture System
… of embryoid bodies (EBs) in a liquid medium and hanging drop system. The OP9 co-culture system did not appear to give rise to well differentiated lymphoid lineage cells during 12 days of differentiation. The hanging drop EB culture system, previously shown to promote differentiation of …
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Drug Discovery in Redox Biology: From Natural Products to Structural Biology
… and FAD incorporation. Crystals obtained via hanging-drop vapor diffusion diffracted to ~4.0 Å at the Advanced Photon Source, enabling visualization of the enzyme’s overall fold and catalytic redox center, supporting structure-guided inhibitor development.
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Serial and Single Crystal Studies of Ligand Binding to Radiation Sensitive Haem Protein DHP-B
… protein data bank. Crystals were grown using the hanging-drop vapour diffusion method, soaked in cryoprotectant and measured at synchrotron sources. Serial data was collected using microcrystals at SACLA, Japan and from DLS. The structures were solved using both CCP4i2 suite and Phenix. The …
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Development of advanced 3D tissue models and O2 imaging methodologies
… which method of formation from "free floating", "hanging drop" and LipidureTM, produced the most uniform, viable and metabolically active in vitro tissue. Numerous applications were improved and aided by the new O2 measuring platforms. Combining the novel probes with our new 3D models we could …
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Biomimetic and vascularized 3-d liver cancer model
… are embedded together on Matrigel. Using the hanging drop method, spheroids are formed and encapsulated in collagen before exposure to the anti-cancer drug doxorubicin. Cell viability, bile canaliculi, and cytochrome p450 activity are measured afterwards, showing greater maintenance of liver …
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A study on the role of oxidative stress and protein kinase signalling in hyperglycaemia induced cardiac remodelling
… pulsatile embryoid bodies (EBs) using the hanging drop method. Once pulsatile, EBs were further cultured for 72 hours in either baseline (25mM) or high glucose (50mM) media or with the pro-oxidant hydrogen peroxide (100μM). Changes in EB morphology and beating characteristics were observed …
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Effect of Cell-Substrate Interactions on Epithelial Cell Mechanics
… in the absence of any supporting structures in hanging drops. We found that the direction of strain in the unsupported monolayers was not correlated to nuclear alignment as observed when the monolayers were grown on soft deformable gels. It was also observed that both gel and glass substrates …
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Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells
… stress delivered prior to the</p> <p>start of hanging drop culture was remembered by mESC, suppressing differentiation</p> <p>events slated to occur from 1-6d later. Mesoderm marker, Brachyury, and anterior</p> <p>visceral endoderm marker, Goosecoid, expression was suppressed. The timing of</p> …
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C/EBPβ Regulates Tumour Size and Microenvironment Composition in Lewis Lung Carcinoma
… carcinoma cells grown as adherent monolayers or hanging-drop spheroids revealed a conserved “core spheroid signature” of 1, 527 genes, characterized by enrichment of autophagy-related pathways and suppression of cell-cycle programs. Consistent with this, spheroids exhibited an increase of cells …
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Impact of chemotherapy on the release and function of extracellular vesicles in the pre-pubertal testis
… 13 and 18 weeks gestation and cultured using a hanging drop culture system established in our laboratory previously. Incubation of cisplatin-derived and control EVs were undertaken for eight days and immunofluorescence was undertaken to quantify Sertoli cell number and the number of apoptotic …
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Micro-nano scale diagnostics and therapeutic platforms for personalized medicine
Limited Restriction Lifted for Item 113065 on 2021-11-27T10:15:20Z.