Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"hairy roots"”.
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Metabolic, biochemical and molecular profiling of Catharanthus roseus flower cultivars and transformed hairy roots for monoterpenoid indole alkaloid accumulation /
… bios}l1thesis in Catharanthus rosellS hairy root cultures have been performed by expressing the last step in vindoline biosynthesis [Dcacetylvindoline-4-0- acetyltransferase (DAT)]. Enzymatic profiling studies with transformed hairy roots have confirmed that over-expressing DAT leads to …
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Investigation of Protein-Protein Interactions among Nicotine Biosynthetic Enzymes and Characterization of a Nicotine Transporter
… of nicotine occurs exclusively in the roots of tobacco, yet accumulates in the leaves of tobacco where it is acts as a defense compound to deter insect herbivory. The research detailed in this dissertation addresses two aspects of nicotine physiology in tobacco: 1) an investigation of …
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Repression der cytosolischen GS1 von Zuckerrüben (Beta vulgaris L. var. altissima) durch Antisense-DNA-Konstrukte
… selektiert und regenerierte Feinwurzeln (hairy roots) mit molekularbiologischen und proteinbiochemischen Methoden untersucht. Die verschiedenen unabhängigen Transformanten zeigten im Vergleich zu den Kontrollwurzeln sowohl unterschiedlich starke Reduktionen in den GS1 mRNA- und den GS1 …
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Isotopic labeling of isoflavones from plant cell and organ culture for metabolic tracking in animal models
… fraction from a high isoflavone yielding kudzu hairy root genotype in vitro, which was used to track the absorption, distribution, and elimination of kudzu isoflavones in rats. Pharmacokinetics showed that kudzu isoflavones were rapidly absorbed, distributed into all tissues including bones, …
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Bioreactor design for scaleup of Catharanthus roseus hairy root cultures for production of indole alkaloids
… indole alkaloids, vincristine and vinblastine. Hairy root cultures are an attractive alternative for the production of plant derived secondary metabolites. C. roseus hairy root cultures were established in our laboratory by A. rhizogenes mediated transformation of C. roseus seedlings prior to …
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Combinatorial biochemistry of triterpene saponins in plants
… saponin biosynthesis genes in M. truncatula hairy roots. Three of the generated transgenic hairy root lines were found to accumulate novel molecules, two of which were shown to be novel triterpene saponins, whereas the third line produced a set of novel, non-saponin compounds. Furthermore, …
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Substrate analogs to investigate alkaloid biosynthesis in Catharanthus roseus
… of alkaloid analogs in Catharanthus roseus hairy roots and seedlings. All terpene indole alkaloids are derived from tryptamine and secologanin, which are coupled to form strictosidine by the enzyme strictosidine synthase (STR). Analysis of the tryptamine substrate specificity of …
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Understanding the molecular mechanism of postharvest seed coat darkening in pinto beans
… an elevated level of PAs in pinto bean hairy roots, that constitutively express PvWD9. Finally, a genome-wide analysis for MATE genes and their detailed characterization in common bean identified PvMATE8 as a transporter of PA monomers. PvMATE8 is able to transport …
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A FAMILY OF SMALL SECRETED PROTEINS FROM RHIZOPHAGUS IRREGULARIS CONTRIBUTE TO ARBUSCULAR MYCORRHIZA SYMBIOSIS
… candidate effectors in Nicotiana benthamiana roots showed evidence for an increase in AM symbiosis. Amongst them, one candidate effector, R5, showed expression evidence in R. irregularis when colonizing N. benthamiana. The gene was found to be part of a gene family containing 15 members, most …
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Plant-derived Murine IL-12 and Ricin B-Murine IL-12 Fusions
… form of mouse IL-12 (mIL-12) was utilized. Hairy root cultures, as a fast-growing bioproduction system were developed from high expressers of mIL-12. A purification scheme was developed to purify plant-derived mIL-12 from hairy roots and purified mIL-12 was used to assess IL-12 bioactivity …
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Characterization of an agrobacterial plasmid inducible for transfer by mannopine and evolution of the core replication and transfer functions of repabc plasmids with class i quorum-sensing and transfer systems
… responsible for inducing crown gall tumors and hairy roots on susceptible plant hosts. These plasmids also encode the genes required for production by the plant, and utilization by the bacteria of unique carbon conjugates called opines. Similarly, repABC plasmids in species of Rhizobium confer …