Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"haematopoietic stem and progenitor cells"”.
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Mapping the transcriptional landscape of haematopoietic stem and progenitor cells
Maintenance of the blood system requires balanced cell-fate decisions of haematopoietic stem and progenitor cells (HSPCs). Individual haematopoietic stem cells (HSCs) decide between self-renewal and differentiation and can generate all mature cell types. Cell-fate decisions are made at the …
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Elucidating Cell Fate Regulation of the Bone Marrow Vascular Niche Through Development of Engineered Extracellular Matrices
Haematopoietic stem and progenitor cells (HSPCs) are crucial to the curative treatment of a variety of leukaemias and autoimmune diseases. However, their rapid differentiation and exhaustion within 72 hours ex vivo poses a barrier to efficient treatment. Here, we have developed a culture method …
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Single cell -omic landscapes in normal and perturbed haematopoiesis
… comparator resource for perturbed datasets, and to permit the discovery of novel biological processes in the differentiation of stem cells into mature tissues. Large scale profiling of human cells now yields tissue scale compendia extending to millions of cells. In the field of …
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From Mechanisms of Leukaemogenic Dysregulation to New Candidate Therapeutics
… maintenance of a continuous flow of mature blood cells from a reservoir of specialised haematopoietic stem cells requires regulation from an intricate network of regulatory programmes. Gene regulatory networks are well evolved to control multi-lineage commitment and differentiation while …
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Targeting integrin alpha 5 and focal adhesion kinase to overcome azacitidine resistance in higher risk myelodysplastic syndromes
… bone marrow failure due to profoundly impaired haematopoietic differentiation with higher risk disease commonly progressing to acute myeloid leukaemia. Azacitidine (AZA) and decitabine are the only available therapies for higher risk myelodysplastic syndromes shown to improve the overall …
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Loss of rad51 in zebrafish (Danio rerio): a novel Fanconi anaemia model
… recombination protein, necessary for strand invasion and crossing over. It has recently been designated as a Fanconi anaemia (FA) gene, following the discovery of two patients carrying dominant negative mutations. FA is a hereditary DNA repair disorder characterised by various congenital …
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An investigation of the role of TRIB2 in steady state and stressed haematopoiesis
… after Trib2 ablation under steady state and proliferative stress conditions, including genotoxic and oncogenic stress. At the steady state, we found that TRIB2 loss did not adversely affect peripheral blood cell counts and populations. No detectable significant differences were found in …
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Somatic evolution in human blood and colon
All cancers were once normal cells. They became cancerous through the chance acquisition of particular somatic mutations that gave them a selective advantage over their neighbours. Thus, the mutations that initiate cancer occur in normal cells, and the normal clonal dynamics of the tissue determine …
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Using single cell transcriptomics to characterise endothelial populations in embryonic haematopoiesis
Haematopoietic stem cells (HSCs) possess the unique ability to reconstitute an animal’s entire haematopoietic system. As such, it is inherently interesting to the fields of regenerative medicine and developmental biology to understand how these cells first form in the embryo. Haematopoietic stem …
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The Effects of Methyltransferase Genes on Human Haematopoietic Stem Cell Function
The highly structured and hierarchical haematopoietic system is defined by the existence of a pool of haematopoietic stem cells (HSCs) at the apex, which possess both multipotent differentiation and self-renewal properties. The HSC pool is heterogenous in terms of self-renewal and differentiation …
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Dissecting the early steps of MLL induced leukaemogenic transformation using a new mouse model of AML
To maintain a balanced production of all mature haematopoietic lineages and at the same time secure a stem cell reservoir, intricate regulatory programs have evolved to control multi-lineage differentiation and self-renewal in haematopoietic stem and progenitor cells. Leukaemogenic mutations …
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Investigating the anti-leukaemic effect of tamoxifen in the myeloproliferative neoplasms
… protein CALR (such as CALR<sup>Ins5</sup> and CALR<sup>Del52</sup>), causing the constitutive activation of signal transducer and activator of transcription (STAT) proteins. The selective oestrogen receptor modulator (SERM) tamoxifen (broadly used in ER+ breast cancer) was effective on …
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Dissecting cellular crosstalk and genetic perturbations in haematopoietic regulation
Haematopoietic stem and progenitor cells (HSPCs) proliferate and differentiate to produce a large number of mature blood and immune cells on a daily basis while maintaining themselves as the reservoir for lifelong haematopoiesis. Haematopoiesis is dynamically regulated by activating …
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Deciphering Leukaemogenic Mechanisms through System-Scale Analysis of Single-Cell RNA Sequencing Data
Haematopoietic stem cells are responsible for producing and sustaining the diverse array of cell types present in the adult blood system. This complex process requires the strict regulation of haematopoietic fate decisions and differentiation trajectories in order to maintain a healthy state. …
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Single-cell Protein-Transcriptome Atlas of Haematopoiesis across the Human Lifespan
The haematopoietic system is responsible for the continuous production of stem and progenitor cells, which divide and mature to produce a wide variety of specialised circulating blood cells. Proper regulation of lineage decisions is key to ensure the right balance between the generation of …