Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"hTERT"”.
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The synthetic dosage lethality between hTERT and DNA damage response associated genes
… 85-90% of primary cancer cells overexpress hTERT. Under normal conditions, the expression of this gene is usually tightly repressed. Cells that do not express hTERT will experience telomere shortening during each cellular division, and eventually, the shortened state of the telomeres will …
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Humoral immunity in colorectal cancer : evaluation of the anti-p53 and anti-hTERT auto-antibody responses
… which are overcome by p53 dysfunction (M1) and hTERT re-expression (M2). These two molecular events generate TAA which are recognised by the humoral immune system with a corresponding auto-antibody response. The aim of this thesis was to investigate the significance of the humoral anti-p53 …
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The Design and Development of Artificial Zinc Finger Transcription Factors and Zinc Finger Nucleases to the hTERT Locus
The ability to direct hTERT expression through genetic control or tunable regulatory factors would advance our understanding of the transcriptional regulation of hTERT, and also potentially produce new strategies for addressing telomerase-associated disease. In this work, we describe the …
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Human telomerase reverse transcriptase (hTERT) overexpression modulates intracellular redox balance and protects cancer cells from apoptotic cell death
The human telomerase reverse transcriptase (hTERT) is the catalytic subunit of the telomerase holoenzyme. Evidence is accumulating to link hTERT to activities other than telomere maintenance and immortalization. Here we show that hTERT overexpression not only reduces the basal cellular ROS levels …
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Regulation of Human Telomerase Alternative Splicing
… of the catalytic subunit of human telomerase (hTERT) may be alternatively spliced into 22 different isoforms. Only a small fraction of hTERT transcripts are spliced into the full length isoform, the form capable of being translated into function hTERT with reverse transcriptase activity. If …
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Differenzielle transkriptionelle Regulation der humanen Telomerase in einem zellulären Karzinogenese-Modell am Beispiel des Plattenepithelkarzinoms des Ösophagus
… durch die <br>Aktivierung des Enzyms Telomerase (hTERT). Bisher ist relativ wenig über die <br>Regulation der Telomerase während des Prozesses der malignen Transformation <br>bekannt. Aus diesem Grund haben wir die transkriptionelle Regulation der <br>Telomerase im Prozeß der malignen …
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Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas
… human telomerase enzyme reverse transcriptase (hTERT)-immortalized human corneal epithelial cell line (hTCEpi) transduced with the hygromycin-thymidine kinase gene (HyTK) as a viable cell source for the reconstruction of the corneal surface. METHODS: HyTK cells were cultured in KGM-2 serum-free …
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Targeting Telomerase Overexpression by Synthetic Dosage Lethality
Telomerase reverse transcriptase (hTERT) is upregulated in multiple cancers and accompanied by the evidence suggesting that up-regulation of telomerase expression is linked to tumorigenesis, raised the hypothesis that inhibiting this enzyme is a powerful antitumor strategy. Despite intensive …
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Glioma genetic profiling: The role of DNA repair and telomerase
… tissues and cell lines. Evidence suggests that hTERT was the only gene that was transcribed in all the glioma cell lines and tissues, while absent in the normal. Thus hTERT may represent a simple but reliable biological marker for distinguishing glioma tissues from normal. The hTERT gene is …
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Signaling Pathway Inhibitor Library Screening Reveals B-Catenin/TCF4 as a Novel Telomerase Regulator in Cancer cell lines
… to inhibit TA via transcription regulation of hTERT (human TERT). Activation of the Wnt pathway either by Wnt ligand (Wnt-3a) or LiCl (activates Wnt signaling by inhibiting GSK-3?) treatment as well as overexpression of a constitutively active form of ?-catenin (?-N ?-catenin) up regulated …
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Hemmung der humanen Telomerase Reverse Transkriptase-Expression mittels synthetischer Nukleinsäuren in Harnblasenkarzinomzellen
… Expressionshemmung des potenziellen Targetgens hTERT (humane Telomerase Reverse Transkriptase) mit AS-ODN und siRNAs in BCa-Zellen untersucht. Die Tumorspezifität der hTERT-mRNA-Expression konnte zunächst an tumor- und tumorfreien Gewebeproben von BCa-Patienten gezeigt werden. Die verwendeten …
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A study of gene expression in human normal and carcinogenic cell lines using qRT-PCR
… CYP2EI), phase II (GSTMJ) and immortalisation (hTERT) genes were investigated. There was evidence suggesting that the transcription of CYPJAI, and CYP/A2 is of cell specific since CYPIAJ transcribed in the A549 eell line only, while CYPIA2 was transcribed in the H460 cell line. The present study …
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Repression of telomerase components in isogenic normal, immortal and tumorigenic cells
… holoenzyme include the reverse transcriptase, hTERT, an RNA component (hTR) that has a template region for reverse transcription and the RNA binding and modifying protein, dyskerin. There is evidence indicating that these components may contribute to the survival and/or proliferation of tumour …
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REGULATION OF TELOMERASE EXPRESSION IN STEM CELL REPROGRAMMING
… tissue regeneration, due to their expression of hTERT and their capacity to differentiate into all three germ layers. Interestingly, telomerase is activated during the induction process, accomplished by the exogenous expression of four genes in normal, non-hTERT-expressing fibroblasts. To …
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The Dietary Isoprenoid Perillyl Alcohol Inhibits Telomerase Activity in Prostate Cancer Cells
… We hypothesized that perillyl alcohol regulates hTERT (human telomerase reverse transcriptase) at the translational and post-translational levels via its effects on the mTOR pathway. A rapid suppression of telomerase activity was detected in prostate cancer cell lines (PC-3 and DU145) in response …
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Telomerase : a tumor marker in the peripheral blood of colorectal cancer patients
… is human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase, which is concomitantly associated with telomerase activation during carcinogenesis. Its expression in tumours has been very commonly described using reverse transcriptase-polymerase chain reaction …
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Telomerase Inhibition and Sensitization of Breast Tumor Cells
… and a catalytically active protein subunit (hTERT), synthesizes telomeric repeats onto chromosome ends and is obligatory for continuous tumor cell proliferation, as well as malignant progression of breast cancer cells. Telomerase is an attractive anticancer therapeutic target because its …
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Chimärer anti-Tumor/CD3 zeta-Kette–Rezeptor („T-Body“) für die Therapie von humanen B-Zell-Neoplasien
… Arbeit das „Immortalisierungsskonstrukt“ hTERT zum Einsatz gebracht. Dieses System wurde parallel zu primären Spender T-Zellen auf seine Wirksamkeit und Funktionalität in Antigenspezifischen T-Zellklonen untersucht.Zu Beginn stand die Klonierung des „T-Body“ Expressionsvektors und die …
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Nucleic Acid Scaffold-dependent Proximity-mediated Enzyme Response (NASPER) - A Proof of Concept Study
Telomerase hTERT RNA is overexpressed in around 90% of all cancers but targeting it has been unsuccessful to date due to the inability of this approach to kill telomerase-expressing cells leading to the evolution of telomerase-independent cells. The approach proposed in the thesis (NASPER), aims to …
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