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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"hESCs"”.
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HYDROGEN PEROXIDE-INDUCED CYTOTOXICITY AND OXIDATIVE DNA DAMAGE ON HUMAN EMBRYONIC STEM CELLS AND THEIR DIFFERENTIATED FIBROBLAST-LIKE PROGENIES
Background: Human embryonic stem cells (hESCs) are pluripotent cells derived from in vitro fertilized (IVF) embryos. Because of their origin in healthy human tissues, self-renewability and differentiate capability, hESCs can potentially be adopted as a biological model for toxicity study. To date, …
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Human embryonic stem cells for genotoxicity testing
… explanted cells. Human embryonic stem cells (hESCs) have been validated as a stable and healthy human cell source. This study aimed to investigate the utility of hESCs as a cellular model for genotoxicity testing. Chromosomal analysis by PNA-FISH and mFISH following Mitomycin C treatment …
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Human Embryonic Stem Cells - Constitutive Gene Expression and Differentiation Towards Definitive Endoderm and Posterior Foregut Endoderm
… transplantations, human embryonic stem cells (hESCs) could be an alternative source of transplantable insulin producing cells. hESCs have the unique potential to self-renew and to differentiate to many cell types, presumably also beta cells. In order to generate therapeutically relevant beta …
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Self-Renewal Requirements of Human Embryonic Stem Cells and Their Engraftment Potential in Mouse Blastocysts
<p>Human embryonic stem cells (hESCs) are a unique population of cells derived from a 6 day old human embryo that can be maintained indefinitely in vitro and have the ability to differentiate to all adult cell types. In addition to their potential for cell based therapies in the treatment of …
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TRANSCRIPTIONAL NETWORKS VARIATIONS DURING CELL CYCLE PROGRESSION IN HUMAN EMBRYONIC STEM CELLS
… of the cell cycle in human Embryonic Stem Cells (hESCs). As a consequence, hESCs can only differentiate into endoderm in the Early G1 phase when Cyclin Ds are expressed at low levels. These results show the mechanisms by which the cell cycle defines differentiation propensity of stem cells. …
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Encapsulation as a 3D model for human embryonic stem cell propagation and differentiation
… cells is essential. Human embryonic stem cells (hESCs) have an indefinite replication capacity and can be directed to differentiate into many types of specialized cells. Currently, efficient protocols to generate beta cells rely on a step-wise ontogenic approach which mimics the in vivo …
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Towards hES cell-based therapy of Epidermolysis Bullosa
… stem cells (human embryonic stem cells, hESCs). Undifferentiated hESCs were exposed to a complex microenvironment in organotypic cultures (to mimic conditions for epidermal stem cell development and maintenance); this yielded epidermis-like structures which stained positively for basal …
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Genome-Wide Profiling Unveils Criticial Functions of P53 In Human Embryonic Stem Cells
… regulatory functions in human ESCs (hESCs) that a) oppose pluripotency and b) protect the stem cell genome in response to DNA damage and stress signaling. In mouse ESCs, these roles are believed to coincide, as p53 promotes differentiation in response to DNA damage, but this is …
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Roles of Heparan sulfate in mesendoderm differentiation of human embryonic stem cells
Human embryonic stem cells (hESCs) are remarkable for their ability to self-renew indefinitely and differentiate into any cell type in the human body. The differentiation of hESCs is regulated by intrinsic and extrinsic signals in the stem cell niche. Heparan sulfate proteoglycans (HSPGs) are found …
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Characterizing the Role of CDK2AP1 in Primary Human Fibroblasts and Human Embryonic Stem Cells
… (HDFs)) and human embryonic stem cells (hESCs) and specifically assess its impact on proliferation, self-renewal and differentiation. In the first part of this study, using a short-hairpin RNA (shRNA) approach, we investigated the effect of CDK2AP1 downregulation in HDFs. Outcomes …
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Signalling and transcriptional regulation of human primordial germ cell specification
… be modelled using human embryonic stem cells (hESCs) for in vitro derivation of human PGC-like cells (hPGCLCs). hESCs in conventional culture conditions have very low potential for germ cell fate; this increases significantly if the cells are first cultured in PGC competence-promoting media. To …
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Chondrocyte biomechanics and chondrogenesis
… cartilages with human embryonic stem cells (hESCs). Both of these areas of investigation are motivated by the significant economic and social burdens of cartilage afflictions. Mechanical forces directly influence the biological processes of native chondrocytes; understanding and controlling …
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Determining the Signalling Pathways that Govern Human Naive Pluripotency
… or “primed” human embryonic stem cells (hESCs) rely on FGF and TGFβ signalling for self-renewal, and occupy a developmentally advanced state of pluripotency comparable to mouse EpiSCs. Recent reports demonstrate that a naïve state of human pluripotency can be consistently derived either …
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IN-VITRO VASCULARIZED TISSUE EQUIVALENTS FROM HUMAN EMBRYONIC STEM CELL-DERIVED ENDOTHELIAL AND VASCULAR SMOOTH MUSCLE CELLS
… cells (vSMCs). Human embryonic stem cells (hESCs) are virtually an unlimited source of differentiated cells including ECs and vSMCs. However, current differentiation strategies need improvisation in terms of efficiency, reproducibility, and use of xenogeneic (animal-derived) products. To …
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Materials and biological approach to gene delivery in human embryonic stem cells
… lung fibroblasts and human embryonic stem cells (hESCs). The PEG-b-PVBLG-8 contained a membrane-disruptive, cationic, helical polypeptide block (PVBLG-8) for complexing with DNA and a hydrophilic PEG block to improve the biocompatibility of the gene delivery vehicle. PEG-b-PVBLG-8 diblock polymers …
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FGF signaling in specification of hESC-derived definitive endoderm
… to be established. Human embryonic stem cells (hESCs) not only offer an excellent source for establishment of strategies for future regenerative cell therapies and drug discovery but also offers en excellent experimental assay for understanding human pancreas development. Pancreas originates …
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Stimulations and modulations of chondrogenic differentiation of human embryonic stem cells
By using human embryonic stem cells (hESCs) as the model system, this study aims to understand the full span process of chondrogenesis, and how this process could be regulated by growth factors BMP2 and/or TGFI?1 in a controlled context both in vitro and in vivo. By culturing hESCs in 2-D EB …
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Materials and biological approach to gene delivery in human embryonic stem cells
… lung fibroblasts and human embryonic stem cells (hESCs). The PEG-b-PVBLG-8 contained a membrane-disruptive, cationic, helical polypeptide block (PVBLG-8) for complexing with DNA and a hydrophilic PEG block to improve the biocompatibility of the gene delivery vehicle. PEG-b-PVBLG-8 diblock polymers …
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The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation
Human embryonic stem cells (hESCs) have the potential to grow into almost all types of cells in the human body. This special property makes hESC invaluable for the restoration of tissue function after damage from different diseases like Parkinson's disease, Alzheimer’s disease, stroke, and …
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Investigating the role of the cardiac apelin receptor using human embryonic stem cell derived-cardiomyocytes.
… apelin receptor. Human embryonic stem cells (hESCs) represent a powerful tool for modelling as they are amenable to genetic editing and can be induced to differentiate to any cell type. This study aimed to use hESC-derived cardiomyocytes to examine the role of the apelin receptor in …
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