Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"hESC"”.
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FGF signaling in specification of hESC-derived definitive endoderm
… to be established. Human embryonic stem cells (hESCs) not only offer an excellent source for establishment of strategies for future regenerative cell therapies and drug discovery but also offers en excellent experimental assay for understanding human pancreas development. Pancreas originates …
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The Role and Control of WNT Signalling in an HESC Model of Human Primitive Streak
… I used an in vitro human embryonic stem cell (hESC) micropatterned "gastruloid" system. I first showed that in the human PS there is a conserved BMP → WNT→ NODAL signalling initiation hierarchy, and that WNT is necessary and sufficient for PS formation. Next, I found that structured …
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INVESTIGATING THE IMMEDIATE CONSEQUENCES OF NORMAL AND MUTANT HTT LOSS IN HD-HESC THROUGH THE DTAG SYSTEM.
… aimed at generating a human embryonic stem cell (hESC) HD model in which normal or mutant HTT can be rapidly and efficiently removed upon exposure to a small molecule. This is made possible by a degradation tag (dTAG)28 that, when fused to HTT, induces a proteosome-mediated degradation upon …
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The Epicardium as the Conductor of Cardiovascular Cells in Cardiac Regeneration
… co-transplantation of human embryonic stem cell (hESC)-derived epicardial cells (EPI) with hESC-derived cardiomyocytes (CM) improves cardiac repair processes with respect to engraftment, cell maturation, and graft and host vascularisation. One plausible explanation for the observed benefits of …
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Cellular therapy for chronic heart failure: a key role for epicardial fibronectin
… damaged heart with human embryonic stem cell (hESC)-derived cardiomyocytes. Recently, outcomes were improved by co-delivering hESC-derived epicardium. Clinically, the main challenge remains chronic heart failure. However, hESC-cardiomyocytes alone, in the chronically infarcted heart, have shown …
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Development of a bioreactor imaging system for characterizing embryonic stem cell-derived cardiomyocytes
… heart is limited, human embryonic stem cells (hESC) have emerged as a source for potential cardiac therapies. However, despite the use of a variety of biochemical differentiation protocols, current yields of hESC-derived cardiomyocytes (CM) have been low. In the case of hESC-CM, which are …
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The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation
Human embryonic stem cells (hESCs) have the potential to grow into almost all types of cells in the human body. This special property makes hESC invaluable for the restoration of tissue function after damage from different diseases like Parkinson's disease, Alzheimer’s disease, stroke, and …
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Investigating the role of the cardiac apelin receptor using human embryonic stem cell derived-cardiomyocytes.
… apelin receptor. Human embryonic stem cells (hESCs) represent a powerful tool for modelling as they are amenable to genetic editing and can be induced to differentiate to any cell type. This study aimed to use hESC-derived cardiomyocytes to examine the role of the apelin receptor in …
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A chemical biology study of human embryonic stem cell pluripotency and differentiation
Human embryonic stem cell (hESC) research provides promising prospects for the future of regenerative medicine. To fully realize its potentials, we not only need to further our understandings toward the molecular mechanisms governing pluripotency and differentiation, but also to find new tools to …
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Identification of molecular signaling pathways underlying human endometrial stromal cell differentiation
… cultures of human endometrial stromal cells (HESC) to analyze the role of estrogen receptor alpha (ESR1) and progesterone receptor isoforms (PRA and PRB) in human decidualization. Previous studies established that HESC, when treated with the differentiation cocktail containing E, P, and a …
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Encapsulation as a 3D model for human embryonic stem cell propagation and differentiation
… cells is essential. Human embryonic stem cells (hESCs) have an indefinite replication capacity and can be directed to differentiate into many types of specialized cells. Currently, efficient protocols to generate beta cells rely on a step-wise ontogenic approach which mimics the in vivo …
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HYDROGEN PEROXIDE-INDUCED CYTOTOXICITY AND OXIDATIVE DNA DAMAGE ON HUMAN EMBRYONIC STEM CELLS AND THEIR DIFFERENTIATED FIBROBLAST-LIKE PROGENIES
Background: Human embryonic stem cells (hESCs) are pluripotent cells derived from in vitro fertilized (IVF) embryos. Because of their origin in healthy human tissues, self-renewability and differentiate capability, hESCs can potentially be adopted as a biological model for toxicity study. To date, …
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Self-Renewal Requirements of Human Embryonic Stem Cells and Their Engraftment Potential in Mouse Blastocysts
<p>Human embryonic stem cells (hESCs) are a unique population of cells derived from a 6 day old human embryo that can be maintained indefinitely in vitro and have the ability to differentiate to all adult cell types. In addition to their potential for cell based therapies in the treatment of …
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Bayesian level sets and texture models for image segmentation and classification with application to non-invasive stem cell monitoring
… stem cells, whether human embryonic (hESC), induced pluripotent (iPSC), or animal. Motivated by this and other applications, the Bayesian Level Set (BLS) algorithm is developed for automated segmentation and classification that computes smooth, regular segmenting contours in a manner …
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The Possible Role for Basigin Receptor Function in The Induction of Matrix Metalloproteinase Expression in Human Endometrial Stromal Cells
… vitro in human endometrial stromal cell lines (HESC). Understanding how basigin induces matrix metalloproteinases within normal cellular conditions will help researchers understand how these signaling cascades flow in the framework of implantation. In order to do this, I attempted to …
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The role of the immune system in Parkinson’s Disease: from neuropathology to treatment.
… characterisation of a human embryonic stem cell (hESC)-derived dopamine neuron progenitor cell (NPC) ahead of its use in the STEM-PD clinical trial for PD. Several groups have shown that hESC-NPCs can generate mature DAn when grafted into rodent models of PD - providing targeted physiological …
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IN-VITRO VASCULARIZED TISSUE EQUIVALENTS FROM HUMAN EMBRYONIC STEM CELL-DERIVED ENDOTHELIAL AND VASCULAR SMOOTH MUSCLE CELLS
… cells (vSMCs). Human embryonic stem cells (hESCs) are virtually an unlimited source of differentiated cells including ECs and vSMCs. However, current differentiation strategies need improvisation in terms of efficiency, reproducibility, and use of xenogeneic (animal-derived) products. To …
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QUANTIFYING OLIGOMERIC STATES OF PROTEINS IN-CELL VIA SINGLE-MOLECULE SUPER-RESOLUTION MICROSCOPY
… we further used CRISPR-Cas9 to generate H1 hESC lines, where the SOD1 gene with genetically tagged with SNAP-tag, allowing us to dissect the oligomeric states of SOD1 at an endogenous expression level. Further, the ability to differentiate hESC to different cell types (e.g., hepatocytes or …
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Chondrocyte biomechanics and chondrogenesis
… cartilages with human embryonic stem cells (hESCs). Both of these areas of investigation are motivated by the significant economic and social burdens of cartilage afflictions. Mechanical forces directly influence the biological processes of native chondrocytes; understanding and controlling …
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Development of novel diagnostics and therapeutics for amyotrophic lateral sclerosis
… and 3D scaffolds for human embryonic stem cell (hESC) and neuronal encapsulation.A Gd-TTC conjugate was developed and characterized that was shown to be highly visible under MRI and preserved the functionality of the native TTC protein in vitro. Live animal MRI imaging and immuno fluorescent …
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