Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 275 for “"guanine"”.
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Regulation of transcription initiation at guanine + cytosine-rich promoters.
Regulation of transcription initiation at guanine + cytosine-rich promoters.
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Analysis of sequence-selective guanine oxidation by biological agents
… cancer, chronic degenerative diseases and aging. Guanine is the most frequently oxidized base in the DNA, and generation of a guanine radical cation (G'") as an intermediate in the oxidation reaction leads to migration of a resulting cationic hole through the DNA n-stack until it is trapped at the …
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The biochemical and molecular basis of Hypoxanthine-guanine phosphoribosyltransferase deficiency
Hypoxanthine-guanine phosphoribosyltransferase (HPRT) catalyses the first step in purine salvage. A complete deficiency of the enzyme results in the devastating neurological symptoms of the Lesch-Nyhan syndrome. The Lesch-Nyhan syndrome is characterised by purine overproduction leading to, …
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Singlet Oxygen Oxidation of Guanine, 9-Methylguanine and Guanine-Cytosine Base Pair: Dynamics and Kinetics Revealed by Parallel Gas- and Solution-Phase Experiments and Computations
… mutagenesis, carcinogenesis, and cellular death. Guanine is the most susceptible DNA target of <sup>1</sup>O<sub>2</sub>. The related process has been studied over three decades but the mechanism has remained elusive. My thesis research has focused on reaction mechanism, dynamics and kinetics of …
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Rationalising the effect of guanine-based Ricin inhibitors using computer simulations
for humans.This has lead to its use as a biological weapon and a therapeutic agent. the reaction mechanism of ricin is not known and so rational design is limited, resulting in no effective inhibitor being found for this enzyme. An important step towards understanding the reaction mechanism and the …
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The expression and drug targeting of parasitic hypoxanthine-guanine phosphoribosyltransferase (HGPRT)
… falciparum, and Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HPRT) in E. coli using the pET expression system. The cDNA encoding the ORF of HPRT was amplified by PCR and transformed into E. coli cells using standard methods. Expression was induced by IPTG and reached about …
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Regulation of rho guanine nucleotide exchange factors through lipid binding and phosphorylation
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-08-01
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Zyxin signaling: Protease-activated receptor-1 and Rho guanine nucleotide exchange factor p114.
… signaling: Protease-activated receptor-1 and Rho guanine nucleotide exchange factor p114.
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Genomic Analysis of Human and Mouse Guanine-7-Methyltransferase with Active Site Characterization
… The methylation of the 7-position on the guanine cap is very important to proper mRNA processing and initiation of translation. The enzyme responsible for this methylation, RNA guanine-7-methyltransferase, has been cloned and studied from a number of different species, including human, X. …
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The effect of guanine nucleotides on glucagon-sensitive adenylate cyclase in the rat heart
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Invesitagating the role of the guanine nucleotide exchange factor P-Rex1 in cell invasion
… of the actin cytoskeleton. P-Rex1, a Rac guanine nucleotide exchange factor (GEF), was previously identified in this lab in a transcriptional screen for mediators of cell invasion. A number of GEFs have been shown to regulate the invasive phenotype of tumours and cancer cell lines, leading …
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Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT)
The research concerns sub-cloning the gene for HGXPRT from Plasmodium falciparum from a vector with a His-tag facility to one without, expression of the protein in E. coli, and purification. On an analytical scale (40 ml culture), a purification procedure was developed that involves extraction of …
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Identification of a family of cAMP-binding guanine nucleotide exchange factors by Gregory M. Springett.
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
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The WXXXE Effector Map Functions as a Potent and Specific Guanine Nucleotide Exchange Factor for CDC42
… activity of GTPases is the conversion of bound guanine triphosphate (GTP) to guanine diphosphate (GDP) via the hydrolysis of the gamma phosphate. The oscillation between the "off" or GDP-bound form and the "on" or GTP-bound form is facilitated by the action of GTPase activating factors (GAPs) …
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Identification and functional characterisation of RAM, a novel and essential component of RNA guanine-7 methylation
… of the guanosine cap, is catalysed by the RNA guanine-7 methyltransferase, RNMT.<br/>RNA guanine-7 methylation is an essential process for cell viability and its up-regulation has been associated with cell transformation. However, the mechanistic details of RNMT function in mammalian cells …
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Purification, characterization and substrate specificity of a nuclear mRNA (Guanine-7)-Methyltransferase from Ehrlich ascites cells
… known as the "cap" structure. The guanine-7 methylation of this cap structure is important for mRNA processing and initiation of translation. A RNA (guanine-7)-methyltransferase that specifically methylates the 5'-terminal guanosine residue of the RNA cap structure has been purified …
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Allosteric Determinants of Guanine Nucleotide Binding Proteins and Methods to Crystallize the Cytosolic Domains of Adenylyl Cyclase
The cytosolic domains of mammalian adenylyl cyclases, termed C1 and C2, are responsible for catalytic activity and most regulatory properties. Crystal structures of the soluble catalytic core of adenylyl cyclase bound to activators Gsa and forskolin were previously determined. However, structural …
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Homology-Directed Repair of Dna Lesions Generated by the Mismatch Repair Dependent Processing of Cytotoxic Guanine Adducts
… adducts, while a thiopurine antimetabolite 6-thioguanine (6-thioG) generates S6-MeG DNA adducts. Instead of pairing with cytosine, these modified guanines mispair with thymine during DNA replication. The Mismatch repair (MMR) pathway rectifies base mismatches. However, MMR is unable to correct …
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