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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"grp78"”.
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Targeting GRP78 in Cancer with Nucleic Acid Bioconjugates
… 2 of this thesis. This bioconjugate exhibited GRP78 oncogene binding affinity (K<sub>D</sub>: 0.25 mM) as characterized by PAGE gel shift assays. Its binding affinity towards the GRP78 oncogene was also confirmed using circular dichorism spectroscopy, and thermal denaturation experiments. …
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Cell-Surface GRP78 and its Antibodies: Pathologic and Therapeutic Roles in Cancer
<p>The chaperone protein GRP78 is primarily expressed in the endoplasmic reticulum, but it is also aberrantly expressed on the surface of cells under pathological conditions. One the cell membrane, GRP78 acts as a signaling molecule with unique properties. The amino-terminal domain acts as a growth …
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Study of the Cellular Role of GRP78/BiP mono-ADP-ribosylation in UPR and Cancer
… to demonstrate that the ER-localized chaperone, GRP78/BiP, was a prime target of hARTC1. A doubly mutated hARTC1 mutant was designed, and used as a specific control for hARTC1 expression. The mutant enzyme localized to the ER, but did not catalyze GRP78/BiP ADP-ribosylation. The demonstration …
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SREBP-1 and Cell Surface GRP78 are important modulators of TGF-β1 in the progression of diabetic nephropathy
… of this thesis is that SREBP-1 and cell surface GRP78 are novel regulators of TGF-β1 signaling in mesangial cells. Our first study aims to define a novel pathway by which SREBP-1 regulates TGF-β1 signaling in kidney mesangial cells. Our results indicate that SREBP-1 regulates the expression of …
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RNA interference targeting Glucose-Regulated-Protein 78 induces HepG2 cell Apoptosis
… protein, 78-kDa glucose-regulated-protein (GRP78), has been observed on the surface of cancer cells, but not on normal tissues. By selectively targeting GRP78 with short-interfering RNA (siRNA), potent GRP78 silencing is anticipated by the RNA interference (RNAi) pathway. Silencing GRP78 …
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Branching into RNAi: Synthesis, Characterization and Biology of Branch and Hyperbranch siRNAs
… the Glucose Regulated Protein of 78 kilodaltons (GRP78) which signals tumor initiation, proliferation and resistance towards chemotherapy. Moreover, GRP78 is overexpressed and cell surface localized on a wide range of resilient tumor types but not on healthy cells, making it a viable bio-marker …
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Poly(Arginine) Derived Cancer-Targeting Peptides for the Development of a Cancer-Targeted Gene Therapy Approach in HepG2 Liver Cancer Cells
… surface receptor, Glucose Regulated Protein 78 (GRP78). GRP78 is a member of the heat shock family of chaperone proteins, assisting in protein folding events under physiological stress induced conditions that are mitigated by the unfolded protein response (UPR) mechanism. In cancer, GRP78 is …
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Regulation of autophagy, endoplasmic reticulum and unfolded protein response by glucocorticoids in physiology and disease
… resulted in increase of ER chaperone proteins GRP78 and GRP94 in CEM-C7-14 cells. This is potentially related to increased cell death and may play a role in ER stress mediated cell apoptosis. Protein degradation inhibition by BTZ sensitised the ALL cells to the treatments and combination …
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Molekulare Mechanismen der Aldosteron-Wirkung und Interaktionen des Mineralocorticoid-Rezeptors in Nierenzellen
… beschriebene Interaktionspartner (p23, 14-3-3, GRP78, Elongationsfaktoren eEF1 und eEF2). Daneben wurden zahlreiche neue Proteine im MR-Komplex identifiziert. Um die differentiellen Wirkungen unterschiedlicher MR-Liganden zu verstehen, wurde die Liganden-abhängige Rekrutierung von Kofaktoren …
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Induktion von myokardialem ER-Stress durch biomechanische Last und neurohumorale Stimulation.
… ER-Chaperonen 78kDa-glucose-regulated-protein (Grp78) und Calreticulin (CRT) in den rechten (RV) und linken (LV) Ventrikeln von Monocrotaline-behandelten Ratten und rechtsventrikulären Muskelstreifen, die verschiedenen Lastbedingungen und pharmakologischen Interventionen ausgesetzt wurden. …
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Role of Endoplasmic Reticulum Stress in Trophoblast Stem Cell Differentiation
… TE stem cell pool, and increased expression of Grp78, Atf4 and Atf6a UPR markers. The phenotype was partially rescued by addition of a chaperone to the culture medium. In conclusion, both the in vitro and in vivo models demonstrate that ER stress perturbs differentiation of TS cells at the …
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Super Low Dose Endotoxin Exacerbates Low Grade Inflammation through Modulating Cell Stress and Decreasing Cellular Homeostatic Protein Expression
… inducing homeostatic molecules such as XBP1 and GRP78/BiP. We observed that cells challenged with SLD endotoxin have significantly reduced expression of homeostatic molecules XBP1 and BiP. Mechanistically, we observed that SLD-LPS increases phosphorylated HCK expression in TM treated cells. …
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Identification of novel determinants of primary and acquired resistance to EGFR-targeted therapies in colorectal cancer
… demonstrated a potential prognostic role for GRP78 expression within a cohort of 156 stage II/III CRC patients. We identified that KRASMT patients with high GRP78 expression had a significantly lower 5yr survival rates compared to KRASMT patients exhibiting low levels of GRP78 expression, …
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Tissue inhibitor of metalloproteinase-1 contributes to ovulatory dysfunction in a rat model of endometriosis
… and rTIMP1 co-immunoprecipitated TIMP1 with GRP78 and GSTA3 which are both involved in protein protection. Ala-TIMP1 complexed more to CD63 than rTIMP1 showing a MMP-independent mechanism to apoptosis inhibition. Together these data show endometriotic TIMP1 role in preventing normal …
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Characterization of Palmitic Acid Induced Lipotoxicity in Schwann Cells
… ER stress signature genes such as CHOP, Xbp1 and GRP78. In SC cultured in euglycemic conditions, lysosomal membrane destabilization preceded mitochondrial membrane depolarization, oxidative stress and caspase 3/7 activation. The release of cathepsin L and B were also observed with PA treatments. …
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Untersuchungen zur Funktion der Protein-Tyrosin-Phosphatase PTPRR
… wurden die Hitzeschockproteine HSP70 und GRP78 als in vitro Bindungspartner der PTPRR identifiziert. Die nähere Untersuchung der Bindung ergab, daß diese innerhalb der Juxtamenbrandomäne der PTPRR erfolgt und von ATP abhängig ist. Diese Art der Bindung ist ein Anzeichen dafür, daß die …
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Molecular mechanisms involved in the anticancer activity of BISPMB in oesophageal cancer cells
… the expression of ER stress and UPR genes ATF4, Grp78 and CHOP in WHCO1 cells. We also observed a decrease in ATF6 90 kDa protein and transient XBP-1 mRNA splicing. The activation of p38, JNK and ERK MAPK pathways in bisPMB treated WHCO1 cells was also observed. Furthermore siRNA mediated …
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RNAi Nanotechnology: A Platform for siRNA Screening and Cancer Gene Therapy
… cancer cells known to overexpress tumorigenic GRP78 activity, the self-assembled siRNAs targeting multiple sites of GRP78 mRNA demonstrated more potent and long-lasting anticancer activity relative to their linear controls. Extending the scope of our RNAi screening approach, the self-assembled …
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Uncovering Reversible AMPylation of BiP Mediated by dFic During ER Homeostasis
… we identified an ER molecular chaperone BiP/GRP78 as a novel substrate for dFic-mediated AMPylation. BiP was predominantly labeled with AMP by dFic in S2 cell lysate. AMPylation of BiP decreases during ER stress but increases upon the reduction of unfolded proteins. Both dFic and BiP are …
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Uncovering Reversible AMPylation of BiP Mediated by dFic During ER Homeostasis
… we identified an ER molecular chaperone BiP/GRP78 as a novel substrate for dFic-mediated AMPylation. BiP was predominantly labeled with AMP by dFic in S2 cell lysate. AMPylation of BiP decreases during ER stress but increases upon the reduction of unfolded proteins. Both dFic and BiP are …
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