Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"granule formation"”.
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Studies on the mechanism of polyhydroxybutyrate (PHB) granule formation in Ralstonia eutropha H16/
… produced by bacteria in the form of insoluble granules for carbon storage. Insights into the mechanisms of granule formation are important for improving commercial PHB production and engineering new alternatives to petroleum-based plastics. Using both biochemical and imaging methods, we …
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The role of the phasin PhaP in promoting polyhydroxybutyrate (PHB) granule formation in Caulobacter crescentus
… polymers. Bacteria package PHB as insoluble granules within the cell, maintaining the amorphous nature of the polymer, which is necessary for its degradation and reutilization when the environment becomes more conducive to growth and division. We are interested in understanding how the …
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Studies on the fluidised bed granulation process.
… the objectives of elucidating the mechanism of granule formation and identifying the fundamental parameters affecting granule quality thereby enabling the true benefits of the process to be realised. Initially the effect of seven process variables upon granule quality was quantified using a …
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Investigation Of Posttranscriptional Regulation After Global Brain Ischemia And Reperfusion Injury
… through a novel cytoplasmic structure, the mRNA granule. To determine the roles of HuR and the mRNA granule in reperfused neurons and to characterize global translation regulation, we 1) prevented mRNA granule formation in reperfused neurons or induced mRNA granules in uninjured neurons by …
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Tvorba proteinových granulí v diferencovaných buňkách kvasinkových kolonií
… processes and stress resistance. Protein granules are membrane-less "organelles" found in both unicellular and multicellular eukaryotic organisms. The formation of protein granules is related to the physiological state of the cell (e.g. chronological and replicative aging), but also to …
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STRESS GRANULES MODULATE SYK TO CAUSE MICROGLIAL DYSFUNCTION IN ALZHEIMER’S DISEASE
… lab indicates that SYK can associate with stress granules, ribonucleoprotein particles that form in stressed cells and contain inactive translation initiation complexes. I found that microglial cell lines and primary mouse brain microglia, when stressed by exposure to sodium arsenite or Aβ(1-42) …
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Regulation of Murine Mast Cell Homeostasis by TGF-β1 and CD4+CD25+C Regulatory T Cells
… c-Kit, T1/ST2 and FcεR expression, and inhibited granule formation in developing mast cells. Accessory cells were not required for this inhibition. Smad3-deficiency did not alter the response of bone marrow cells to TGFβ1. TGFβl inhibited expression of the FcεRI a subunit protein, without …
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Ruolo della TDP-43 nella formazione dei granuli da stress nella Sclerosi Laterale Amiotrofica
… studies suggest that TDP-43 may regulates stress granules dynamics that are cytoplasmic structures composed of non-translating messenger ribonucleoproteins (mRNPs) that rapidly aggregate in cells exposed to adverse environmental conditions. Stress granules function in part to triage RNA and …
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An omics study into the molecular impact of autosomal dominant APP and MAPT mutations on the cerebral cortex
… patients carrying MAPT E10+16 mutation suggests formation of Nop58+ inclusions in the grey matter of these patients, potentially related to stress granule formation. The role of Nop58 in the brain or in tauopathies has not yet been described. The results from my dissertation provide a platform …
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Mechanistic investigation of polyhydroxybutyrate (PHB) synthases and elucidation of PHB biosynthesis and degradation process in Wautersia eutropha H16
… initiation and the elongation phases leading to granule formation. For the first time, we have observed intermediate species in the in vitro reaction containing a mutant Class III synthase, D302A-PhaCPhaEAv, with its natural substrate (R)-3-hydroxybutyryl-CoA (HB-CoA). Analysis of reaction …
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Yeast Ataxin-2 (Pbp1) Condensates Regulate TORC1 Activity and Autophagy in Response to Cellular Redox State
… that has earlier been implicated in stress granule formation, RNA biology, and neurodegenerative disease. However, the normal endogenous function of Pbp1 and ataxin-2 remains poorly understood. In this dissertation, I identified Pbp1 as a dedicated regulator of TORC1 signaling and autophagy …
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RNA-binding proteins mediate anti-inflammatory regulation of vascular disease
… EIF2α phosphorylation, requisite for SG formation, was also induced by clotrimazole and oxLDL in these cells. Interestingly, VSMCs pre-treated with anti-inflammatory cytokine IL-19 followed by clotrimazole significantly reduced the formation of SGs and eIF2a phosphorylation, suggesting a …
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Role of Syk in the regulation of cytoskeleton and stress granules in breast cancer
… partners also found to be recruited to stress granules. Treatment of cells with inducers of stress granule formation leads to the recruitment of Syk to these protein-RNA complexes. This recruitment requires the phosphorylation of Syk on tyrosine and results in the phosphorylation of proteins at …
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Elucidating the Role of RanBP2/Nup358 in the Regulation of Cytokines mRNAs
… we uncovered a novel role for RanBP2 in stress granule dynamics. We showed that RanBP2 SUMOylates G3BP2, a core stress granule component, to delay stress granule formation during stress and promote their disassembly during recovery. Since many viruses target stress granules to evade immune …
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Molecular mechanisms underlying morphological effects of protein kinase C under normal conditions and cellular stress.
… proteins were found to localize to specific RNA-granules, formed when cells are exposed to stress. PKCalpha, rather than PKCε, was found to be the main PKC isoform found in these stress granules. We have furthermore discovered a role for PKCalpha in regulating stress granule formation.
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Molecular Dissection of a Caenorhabditis Elegans Germ Granule Assembly Protein to Determine Its Role in Cell Development
… of animal life. Germ cells contain germ granules, cytoplasmic non-membrane bound organelles also referred to as RNA-protein condensates. These germ granules house a variety of RNAs and proteins essential for germline identity, maintenance, and fertility. In C. elegans, P granules are a …
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Regulation of cellular growth and identification of stromal gene signatures in breast cancer
… complexes of proteins and RNAs, called stress granules, to inhibit translation of proteins unnecessary for the cell during harmful conditions and focus translation on stress-related proteins. In neuroblastoma and breast cancer cell lines, we have found that the protein kinase Cα (PKCα) isoform …
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Stress-Induced Targeting of Molecular Chaperones In The Yeast Saccharomyces Cerevisiae
… in sporulating diploid cells also blocked spore formation, underscoring the importance of this chaperone in this developmental program. The yeast molecular chaperone Hsp104 is a member of the Hsp100 superfamily of AAA+ ATPases. Unlike the Hsp90 family of chaperones, Hsp104 is not restricted to a …
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Mechanisms of dynein-based mRNA transport processes in human cells
… As FXR1 and FXR2 are highly enriched in stress granules – large RNPs that sequester mRNAs when cells receive an insult – this thesis also explores the role of the dynein-BICD2 complex in the assembly of these structures. For this purpose, biochemical assays, as well as fixed and live cell …
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Einfluss von Prozessoptimierungen auf die mikrobielle Diversität und die Effizienz der Gasbildung in Co-Vergärungsanlagen der Abfallwirtschaft
Im Hinblick auf die Problematik der Umweltverschmutzung durch die Nutzung fossiler Brennstoffe ist es nötig, eine langfristig stabile und umweltfreundliche Energieversorgung zu gewährleisten. Eine Möglichkeit, den Energiebedarf CO2-neutral zu decken, ist die Nutzung von Biogas. Hierbei spielt der …