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Showing 1 to 20 of 34 for “"gp41"”.
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Solid-state NMR investigation of viral fusion glycoprotein 41 (gp41)
… changes of the viral fusion protein gp41. Our current understanding of the mechanisms of protein-induced membrane structural changes involved in viral entry is incomplete because the hydrophobic N-terminal fusion peptide (FP) and C-terminal transmembrane domain (TMD) of gp41 have …
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HIV entry : a biophysical and mutational analysis of gp41-mediated membrane fusion and its inhibition
… two non-covalently attached subunits, gpl20 and gp41, that associate as a trimer on the virion surface. Once gp 120 contacts the target cell, gp41 undergoes extensive conformational changes to mediate fusion of viral and cellular membranes. First, a short hydrophobic stretch of residues at the …
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Transmembrane glycoprotein gp41 of the Human Immunodeficiency Virus Type I : gene synthesis, recombinant expression and immunological characterization
… an effective strategy to combat HIV/AIDS. Gp41 might be of interest for vaccine development because of its antigenic neutralizing epitope sites previously discovered. Here, the ecto- and endo-domains of HIV-1 gp41 were studied. The endo-domain was truncated into three gene fragments namely …
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Crystal structure of a mutation in the HIV gp41 core : effect on a deep cavity and implication for drug development
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Physics, 1999.
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Characterisation of HIV-1 subtype C envelope functional determinants of dual infected individuals
… Changes in Env fusogenicity indicated that gp41 might be a fitness determinant of PSV entry efficiency and analysis of SGA sequences indicated that recombination within gp41 was common to 3/4 participants. Env chimeras were generated where gp41 was swapped between clones that either had the …
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Untersuchungen zum zeitlichen Verlauf der Antikörperavidität bei HIV-1-infizierten Patienten mit antiretroviraler Therapie
… : Bei insgesamt 39 HIV-Patienten wurden die Anti-gp41-, Anti-gp120- und Anti-p55-IgG-Avidität von Proben aus den Jahren 1991-2001 in ein- bis dreijährigem Abstand bestimmt. Auf der Basis des Infektionsverlaufes wurden die Patienten in vier Gruppen eingeteilt : 1. Langzeitüberlebende (LTS; n=12); …
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Design and characterization of novel immunogens for AIDS vaccine development and evaluation of a sample inference method for NGS Illumina amplicon data
… The membrane proximal external region (MPER) of gp41 and the CD4 binding site (CD4bs) of gp120 have become attractive targets for vaccine development because they contain highly conserved epitopes recognized by some of the broadest neutralizing antibodies. Here, we have designed and characterized …
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Proteins Inside Cells and On the Surface of Membranes: Developing Three Dimensional In-Cell NMR and Structural Characterization of a Trimeric Membrane Proximal External Region Construct from HIV-1 gp41
… region (MPER) of the HIV-1 envelope protein, gp41. This region is membrane associated and an important target for HIV-1 vaccine development because it contains the epitopes for several of the broadly neutralizing antibodies against HIV-1. In addition to studies of the monomeric peptide, we …
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HIV-1 Envelope-Induced Signaling Mediates Actin Cytoskeleton Rearrangements Necessary for Fusion and Entry
… subunit gp120 and the transmembrane subunit gp41 which anchors gp120 into the viral membrane. First the surface subunit gp120 binds to the primary receptor CD4. This interaction promotes actin cytoskeletal rearrangements in the target membrane that bring the chemokine coreceptor, CCR5 or …
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Inhibiting HIV-1 entry : utilizing a transient intermediate of viral membrane fusion as a target for drug discovery
… therapy. After virion attachment to the cell, gp41 undergoes a conformational change and inserts into the target cell membrane. Concomitantly, a conserved region of gp4 l that is hidden both before and after this stage is accessible. The exposed core contains a trimeric coiled coil. A …
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Evaluating the neutralizing antibody response to HIV-1 membrane proximal external regional; Implications for vaccine design
… External Region (MPER) within the HIV- 1 gp41 envelope is a promising vaccine target. The MPER is highly conserved, functionally constrained, facilitates virus fusion and is targeted by broadly neutralizing monoclonal antibodies. The objectives of this research were 1) To evaluate the …
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The Design And Synthesis Of Inhibitors Of Hiv-1 Viral Entry And Photoaffinity Labeled Probes For Structural Elucidation Of The Hiv-1 Env
… molecule warhead tethered to a segment of the gp41 MPER denoted Trp3, have achieved irreversible lytic inactivation of HIV-1 virions. Finally, small molecules appended with photoactivatable crosslinking moieties were developed which stabilize the Env conformation recognized by most broadly …
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HIV Specific B Cell Response in Patients With Broadly Neutralizing Serum Activity
… region of gp120 as the CD4 binding site and to gp41. In four patients highly potent broadly neutralizing antibody clones directed to the CD4 binding site were isolated. Despite extensive hypermutation, these broadly neutralizing CD4bs antibodies shared a consensus sequence of 68 IgH chain amino …
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Configurationally restricted bis-tetraazamacrocyclic complexes: chemokine receptor antagonists
… replication by blocking the interaction of gp!20/gp41 with the protein. The incorporation of transition metals (e.g. zinc(II) and copper(II)) into the macrocyclic cavity increases anti-viral potency. The addition of a bridging ethylene unit to the macrocyclic framework locks the complex into a …
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Charakterisierung membranverankerter HIV-Fusionsinhibitoren
Die von gp41 abgeleiteten Peptide T-20 und C46-B inhibieren die für den HIV-Eintritt essentielle Fusion der viralen mit der zellulären Membran. Der in dieser Arbeit durchgeführte Vergleich der antiretroviralen Aktivität der membranverankerten Fusionsinhibitoren C46-A, C46-B und C46-C sollte zur …
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HIV-1 Immune Escape and Neutralizing Antibodies : Solid Phase Proteoliposomes Containing HIV-1 Envelope Glycoproteins as Antigens and Immunogens and HIV-1 Core Envelope Glycoproteins Deficient in T-Helper Epitopes
The HIV-1 envelope glycoproteins gp120 and gp41 mediate binding and fusion of the virus to target cells. The envelope glycoproteins are exposed on the surface of the virus as trimeric spikes and are the major targets for neutralizing antibodies. The design of envelope glycoprotein-based subunit …
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Probing surface protein patterning in biological systems using fluorescence nanoscopy
… In HIV-1, envelope proteins gp120 and gp41 are known to play a significant role in infectivity and their arrangement is indicative of internal viral structure at different stages of its life cycle. We ascertained the distribution of both the envelope proteins on surface of virions at a …
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Coordination among proteins, lipids and water in membrane fusion and fission probed by solid-state NMR
… extent to which HIV’s membrane fusion protein gp41, and Influenza A’s membrane fission protein M2, utilize these five processes to carry out their functions. We use solid-state NMR techniques to probe intermolecular interactions along those lines, with an emphasis on curvature and clustering. …
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Characterisation of llama antibody fragments able to act as HIV-1 entry inhibitors
… which consists of trimers of gp120 bound to gp41. Most variants of HIV-1 enter cells through attachment of the envelope spike to the main cellular receptor CD4, allowing interaction with a co-receptor and eventually fusion of viral and cellular membranes. Neutralising antibodies inhibit HIV-1 …
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Structure and dynamics of membrane proteins from solid-state NMR
… the TMD of two fusion proteins, PIV5 F and HIV gp41. For the PIV5 TMD, the membrane dependent secondary structure was determined by measuring the chemical shifts: predominant a-helical conformation in the POPC/cholesterol membrane shifts to the [Beta]-strand in the POPE membrane. Using 19F spin …
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