Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"gp160"”.
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EXPRESSION AND PROCESSING OF HIV-1 GP160 WITH HOMOLOGOUS AND HETEROLOGOUS SIGNAL SEQUENCES USING A VSV VECTOR SYSTEM
Previous research has shown that when the natural signal sequence (NSS) of HIV 1 gpl20 was substituted with the honeybee mellitin signal sequence (MSS), the gpl20 became efficiently secreted. However, this work was carried out using the baculovirus expression system in which the processing of gpl20 …
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Untersuchungen zum Transport und Abbau des HIV-1 Envelope Glykoproteins
… einem heterologen, chimären Protein, als auch im gp160 Glykoprotein zu einer Inhibition der Oberflächenexpression und zur Golgi Lokalisation führen. Der Mechanismus konntezumindest teilweise auf eine Internalisierung des auf der Zelloberfläche exprimierten Proteins zurück-geführt werden.
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Herstellung und Charakterisierung eines anti-HIV-Inhibitors basierend auf modifiziertem Serum Albumin
… CXCR4 und CCR5 exprimieren, wurden mit einem gp160-Expressionsvektor transfiziert und die gp120/gp41-induzierte Synzytienbildung beobachtet. Für die Transfektion wurden zwei Expressionsvektoren für ein X4-tropes und ein R5-tropes gp120 (NL4-3, NL-991) eingesetzt. In den beiden Experimenten …
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Refining the HIV-1 glycan shield model: dynamics of a heterogeneous envelope trimer and empirical prediction of glycan processing
… glycosylation of a laboratory determined gp160 strain. A secondary aim was to estimate whether the type of glycan is predictable using computational techniques, since these are less expensive and time consuming than laboratory studies. Using 500 ns molecular dynamics (MD) simulations, it …
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Caractérisation des convertases furine/Pace et Pace4 pouvant être impliquées dans la maturation du précurseur proendothéline-1 humain
… maturer plusieurs précurseurs dont le pro-?-NGF, gp160 de HIV-1, l'antigène protecteur de B. anthracis, le pro-facteur von Willebrand et le pro-TGF?. Les substrats de PACE4 sont inconnus. L'endothéline-1 (ET-1) est un peptide vasoconstricteur très puissant produit par l'endothélium. Composé de 21 …
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SMAC Mimetics Sensitize HIV-Infected Cells to MG1-Mediated Death
… full length or modified versions of HIV envelope gp160 were produced. These pseudotyped viral constructs would have restricted tropism and would only be expected to infect the target cells of HIV. Unfortunately, these viral constructs were unable to be rescued and the clones require further …
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Conformational engineering of human chemokine receptors and HIV-1 Env using deep mutational scanning
Experimental analysis of protein mutations is classically a ‘small data’ problem. A limited number of mutants are made at sites of suspected importance, and the activity of each variant is individually tested. However, by combining directed evolution and deep sequencing with site- saturation …