Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"glyoxylate shunt"”.
-
Functional and mathematical analysis of the glyoxylate shunt in Streptomyces coelicolor
… Based on work done in Escherichia coli, the glyoxylate shunt was thought to be the main anapleurotic pathway in S. coelicolor during growth on fatty acids and therefore an important pathway in providing precursors for secondary metabolism. The S. coelicolor genome contains genes for a second …
-
The glyoxylate shunt as a target for antibacterial intervention in Pseudomonas aeruginosa
… acetyl coenzyme A for biomass production via the glyoxylate shunt. The glyoxylate shunt is comprised of two enzymes: isocitrate lyase and malate synthase G, and allows the synthesis of cellular constituents from C2 nutrient sources, bypassing the decarboxylation steps of the citric acid cycle when …
-
Three enzymes - One substrate Regulation of carbon flux through a "non-canonical" metabolic branchpoint
… gluconeogenesis. They perform this by using the “glyoxylate shunt”. Here, isocitrate is cleaved by isocitrate lyase (ICL) to yield succinate and glyoxylate (which, in a subsequent reaction, is combined with a further acetyl-CoA unit to yield the gluconeogenic precursor, malate). However, ICL has …
-
Glyoxylate Metabolism in Mycobacterium smegmatis
… intervention. Recently, one enzyme of the glyoxylate shunt of M. tuberculosis, isocitrate lyase (ICL), was shown to be required for virulence in experimental infections of mice. The other enzyme of the glyoxylate shunt, malate synthase (MLS), may also be important for the intracellular …
-
Microbial synthesis of 3,4-dihydroxybutyric acid, 3-hydroxybutyrolactone and other 3-hydroxyalkanoic acids
… integrating the 3HA platform with the endogenous glyoxylate shunt. 3HBL has been identified as a top value-added platform chemical from biomass by the US Department of Energy due to its applications as a chiral synthon for a variety of pharmaceuticals, with an estimated wholesale cost of $450/kg. …
-
Quantifying the Intracellular Metabolic Network that Establishes the Simultaneous Utilization of Sugars and Aromatic Substrates in Pseudomonas putida KT2440
… and glucose led to the activation of the glyoxylate shunt. Accordingly, kinetic isotopic flux revealed a decreased flux through isocitrate dehydrogenase and α-ketoglutarate dehydrogenase. In addition, feeding on the substrate mixture induced carbon overflow from the TCA cycle primarily …
-
Microbial Degradation of Acetaldehyde in Freshwater, Estuarine and Marine Environments
… is assimilated into biomass by upregulating the glyoxylate shunt and gluconeogenesis pathway, ensuring carbon is conserved for growth. Using 14C-radiolabelling, rates of microbial dissimilation were shown to be significantly higher than assimilation rates in the freshwater rivers, estuaries, and …
-
Structural and mechanistic investigation towards the regulation of M.tb isocitrate lyase
… tb) during infection. It is a key enzyme of the glyoxylate shunt and the methylcitrate cycle, metabolic pathways that are central to the bacteria’s carbon and lipid metabolism. The absence of ICL in mammals makes it a potential inhibition target for developing new treatments against tuberculosis. …
-
Understanding the molecular mechanism through which aspirated bile triggers chronic Pseudomonas aeruginosa infections in respiratory disease
… apparent. The latter screen identified the glyoxylate shunt as a key breakpoint in the suppression of redox potential as part of the bile response. Bile was also found to be capable of selecting for genetic variants in an in vitro system known to mimic conditions found within the CF lung …
-
Studies on lipoic acid metabolism
… levels of OGDH, the isocitrate lyase of the glyoxylate shunt, and aspartate oxidase, the enzyme catalyzing the first step of nicotinamide biosynthesis. In chapter 4, I characterize the lipoate-protein ligase of the yeast S. cerevisiae. The E. coli LplA was the first lipoate-protein ligase …