Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"glyoxylate"”.
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Glyoxylate Metabolism in Mycobacterium smegmatis
… intervention. Recently, one enzyme of the glyoxylate shunt of M. tuberculosis, isocitrate lyase (ICL), was shown to be required for virulence in experimental infections of mice. The other enzyme of the glyoxylate shunt, malate synthase (MLS), may also be important for the intracellular …
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Poly(ethyl glyoxylate) Solid-Core Particles for Drug Delivery
… Herein, NPs were developed using poly(ethyl glyoxylate) (PEtG) blended with poly(d,l-lactic acid) (PLA) to encapsulate a hydrophobic cargo to be released upon stimulus-triggered cleavage of the PEtG end-cap. The PEtG-PLA NPs were formed using an oil-in-water emulsion-evaporation technique. …
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Functional and mathematical analysis of the glyoxylate shunt in Streptomyces coelicolor
… Based on work done in Escherichia coli, the glyoxylate shunt was thought to be the main anapleurotic pathway in S. coelicolor during growth on fatty acids and therefore an important pathway in providing precursors for secondary metabolism. The S. coelicolor genome contains genes for a second …
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The Role of the Glyoxylate Cycle in the Pathogenesis of Mycobacterium tuberculosis
… prokaryotic- and eukaryotic-like isoforms of the glyoxylate cycle enzyme isocitrate lyase (ICL). The glyoxylate cycle is employed by cells when fatty acids are the main carbon source available. Here, we show that these enzymes are jointly required by M. tuberculosis for growth on fatty acids and …
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The glyoxylate shunt as a target for antibacterial intervention in Pseudomonas aeruginosa
… acetyl coenzyme A for biomass production via the glyoxylate shunt. The glyoxylate shunt is comprised of two enzymes: isocitrate lyase and malate synthase G, and allows the synthesis of cellular constituents from C2 nutrient sources, bypassing the decarboxylation steps of the citric acid cycle when …
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Defining the role of 4-hydroxy-2-oxoglutarate aldolase in hydroxyproline metabolism and primary hyperoxaluria
Aberrant glyoxylate metabolism is a hallmark of primary hyperoxaluria (PH). PH types 1 and 2 are the consequence of functional defects in alanine-glyoxylate aminotransferase (AGT) and glyoxylate reductase (GR), respectively. The resulting increase in oxalate excretion can lead to the formation of …
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Molecular Studies to Investigate 4-hydroxy-2-oxoglutarate Metabolism Defects of Primary Hyperoxaluria Type 3
… affecting enzymes involved in hydroxyproline and glyoxylate metabolism. Of the three PH types, the third type, PH3, is the most recently classified and least thoroughly investigated. PH3 results when mutations prevent 4-hydroxy-2-oxoglutarate aldolase (HOGA) from cleaving 4-hydroxy-2-oxoglutarate …
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GC-MS Investigation of Metabolic Activity in 4-Hydroxy-2-Oxoglutarate Aldolase Deficiency of Primary Hyperoxaluria Type 3
… a group of three variants of an inborn error of glyoxylate metabolism. The hallmark of PH is the overproduction of oxalate which is formed from oxidation of glyoxylate by lactate dehydrogenase (LDH) and which readily crystallizes as calcium oxalate in renal tissue. PH3 is the most recently …
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ANALYSIS OF ANIONS AND PROTEINS RELATED TO OXALATE METABOLISM IN HUMAN ERYTHROCYTES
… presence and investigated the functional role of glyoxylate reductase/hydroxypyruvate reductase GR/HPR), a key protein in the metabolism of oxalate, in human erythrocytes. In addition, the presence of anions associated with oxalate metabolism was investigated using a novel technique that couples …
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Heterologous production of recombinant peptidylglycine α-amidating monooxygenase for the production of biosimilar α-amidated peptides
… assay for measuring the product of PAM activity, glyoxylate, was developed based on a 2-aminobenzaldehyde-glycine-glyoxylate (AGG) absorbance assay. The AGG chromophore synthesised was identified with ultra-performance liquid chromatography mass-spectroscopy (UPLC-MS). PAM activity was measurable …
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Three enzymes - One substrate Regulation of carbon flux through a "non-canonical" metabolic branchpoint
… gluconeogenesis. They perform this by using the “glyoxylate shunt”. Here, isocitrate is cleaved by isocitrate lyase (ICL) to yield succinate and glyoxylate (which, in a subsequent reaction, is combined with a further acetyl-CoA unit to yield the gluconeogenic precursor, malate). However, ICL has …
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The dynamic interplay between DNA damage and metabolism : the metabolic fate and transport of DNA lesions and novel DNA damage derived from intermediary metabolism
… adducts. This concept is illustrated here with glyoxylate from the glyoxylate metabolic cycle, whicvh plays a key role as an alternative to the TCA cycle in plants, bacteria, protists and fungi under changing conditions of environmental nutrients. The goal of this project was to characterize DNA …
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Studies on the metabolism of polyhydroxyalkanoic acid and phosphate in the biological excess phosphate removal process
… Fluoroacetate was used as an inhibitor of the glyoxylate cycle. It seemed that the glyoxylate cycle was not the only source of NADH for PHA synthesis.
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[Beta]- aminothiols and the regulation of hepatic oxalate production / Paul Wayne Baker.
… oxalate production by forming adducts with glyoxylate, the immediate precursor of oxalate. They indicate that cysteine delivery drugs like OTC have the potential to aid in management of calcium oxalate stone disease, thereby reducing endogenous oxalate production and urinary oxalate …
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Structural and mechanistic investigation towards the regulation of M.tb isocitrate lyase
… tb) during infection. It is a key enzyme of the glyoxylate shunt and the methylcitrate cycle, metabolic pathways that are central to the bacteria’s carbon and lipid metabolism. The absence of ICL in mammals makes it a potential inhibition target for developing new treatments against tuberculosis. …
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HYDROXYPROLINE DEHYDROGENASE: A PROMISING TARGET FOR TREATING ALL THREE FORMS OF PRIMARY HYPEROXALURIA
… Hyperoxalurias (PH) are rare disorders of glyoxylate metabolism that cause the formation of calcium oxalate kidney stones. Currently, treatments for PH are limited. Available treatments focus on easing the symptoms of PH, not treating the disease itself. Hydroxyproline metabolism is a …
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Microbial synthesis of 3,4-dihydroxybutyric acid, 3-hydroxybutyrolactone and other 3-hydroxyalkanoic acids
… integrating the 3HA platform with the endogenous glyoxylate shunt. 3HBL has been identified as a top value-added platform chemical from biomass by the US Department of Energy due to its applications as a chiral synthon for a variety of pharmaceuticals, with an estimated wholesale cost of $450/kg. …
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Evaluation of oxidative enzymes in leaf tissue from intact cotton plants exposed to different oxygen concentrations
… superoxide dismutase, catalase, peroxidase, glyoxylate reductase, and acid phosphatase, appeared to be independent of the oxygen treatment. When humidity was carefully controlled at 70%, treatment with 75% oxygen, 350 ppm carbon dioxide resulted in a 180% increase in glutathione reductase, an …
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Regulation of Alternative Carbon Metabolism In Candida Albicans
… in alternative carbon metabolism, including the glyoxylate cycle, β-oxidation and gluconeogenesis. Mutations in key enzymes such as ICL1 (glyoxylate cycle) and FOX2 (fatty acid β-oxidation) revealed that alternative carbon metabolic pathways are required for full virulence in<em> C. …
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Embryonic control of isocitrate lyase activity in the megagametophyte of Ponderosa pine seeds
… isocitrate lyase (threo-L<sub>S</sub> isocitrate glyoxylate lyase, EC 4.1.3.1) activity by a factor that diffuses into the megagametopnyte from the embryo of stratified germinating Pinus ponderosa seeds. In contrast, the data reported here indicate the rate of increase in specific activity of …
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