Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 51 for “"glycosyltransferases"”.
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Investigation of glycosyltransferases from oat
… by the action of family one UDP-dependant glycosyltransferases (UGTs). Glycosylated products of UGTs are known to be essential for reproductive fitness, defence against pathogens, and signalling; UGTs also have a role in the detoxification of xenobiotics. To date, little is known about …
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Essential Glycosyltransferases in Schizosaccharomyces Pombe
I cloned cDNAs encoding S. pombe, human and Caenorhabditis briggsiae Dol-P-Man synthases, all three of which lack a characteristic carboxy-terminal transmembrane region found on previously isolated Dol-P-Man synthases. Known Dol-P-Man synthases can therefore be divided into a 'human' or 'S. …
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Small molecule inhibitors against enteric bacterial glycosyltransferases
… NleB and SseK (NleB ortholog), which are glycosyltransferases that glycosylate protein substrates on arginine residues. This modification is unusual because it occurs on the guanidinium groups of arginines, which are poor nucleophiles, and is distinct from the activity of the mammalian …
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Molecular and biochemical characterisation of novel glycosyltransferases in Mycobacterium tuberculosis
… with features common to the GT-C superfamily of glycosyltransferases. A novel arabinofuranosyltransferase, AftC, was deleted from both M. smegmatis and C. glutamicum and shown to be an internal branching α(1→3) arabinofuranosyltransferase involved in arabinogalactan biosynthesis. Further studies …
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Priming and processing glycosyltransferases in bacterial N-linked glycosylation pathways
… product is then further elaborated by additional glycosyltransferases, flipped across the inner bacterial membrane, and ultimately used as glycosyl donor substrate in transfer to a final acceptor substrate, which, is a lipid, a protein or another glycan. This thesis focuses on the priming GTs that …
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Biochemical and molecular characterisation of cell wall glycosyltransferases in Mycobacterium tuberculosis
… open reading frames, which encode for putative glycosyltransferases from M. tuberculosis and deleted their respective orthologues in C. glutamicum. Mutant phenotypes were characterised biochemically using, two-dimensional-thin layer chromatography, SDS-Polyacrylamide-gel electrophoresis, …
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Sequence-structure-function relationships of glycosyltransferases in families GT43, GT47, and GT64
… enzymes in the Golgi apparatus. Most glycosyltransferases catalyse the formation of unique glycosidic bonds; thus, the activities of these enzymes are responsible for controlling the diversity of glycan structures. The study of glycosyltransferases can therefore grant insight into the …
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Elucidation and Characterization of Glycosyltransferases Implicated in Pectic Polysaccharide Biosynthesis in Arabidopsis thaliana
… to plant cells and the plant as an organism. Glycosyltransferases (GTs) are enzymes that catalyze the transfer of a sugar molecule from an activated sugar-nucleotide donor to an acceptor substrate, and these enzymes are responsible for cell wall polysaccharide synthesis. Across plant and …
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Study of the Dynamics of Retaining GT-B Fold Glycosyltransferases Using Hydrogen Deuterium Exchange Mass Spectrometry
Glycosyltransferases (GTs) facilitate the transfer of sugar moieties from activated sugardonors to various sugar acceptors. Structurally, GTs are classified as GT-A and GT-B folds.While the mechanism of inverting GT-Bs is well-established, the mechanism for retaining GT-Bsis debated. Proposed to …
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Elucidating the role of TM9SF2 in membrane trafficking
… addition of glycans by a group of enzymes called glycosyltransferases. Glycosylation has no template; therefore, it relies on the sequential organisation of glycosyltransferases within the Golgi. The cisternal maturation model proposes that cisternae progress through the Golgi, acquiring the …
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Assembly of the O Antigen in the Highly Pathogenic Escherichia Coli Serotype O104:H4
… the repeating structure is added sequentially by glycosyltransferases. These enzymes catalyze the transfer of a donor sugar from a nucleotide sugar onto a nucleophilic acceptor. Our aim was to biochemically characterize the second, third and fourth glycosyltransferases that synthesize the …
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Targeting final steps of sugar nucleotide biosynthetic pathway against <i>Aspergillus fumigatus</i>
… cell wall carbohydrates is carried out by Leloir glycosyltransferases, which are membraneembedded enzymes that convert sugar nucleotides to cell wall carbohydrates. Therefore, targeting Leloir glycosyltransferases may affect cell wall carbohydrates and in turn elicit antifungal activity, i.e., …
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Chemical tools to probe carbohydrate recognition and glycoconjugate biosynthesis
… where a number of specific membrane-bound glycosyltransferases transfer sugar moiety from soluble or lipid linked activated sugar nucleotides to variety of acceptors with correct stereo and regioselectivity. Many glycoconjugates structures have been elucidated; however, most …
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O antigen biosynthesis in Gram-negative bacteria: Characterization of alpha1,4-glucosyltransferase WclY from Escherichia coli O117 and development of glycosyltransferase inhibitors.
… units of oligosaccharides that is assembled by glycosyltransferases. Many of these bacteria are associated with disease, and the polysaccharides are functionally important for immune responses. In light of increasing antibiotic resistance it is critical to develop new antibacterial strategies …
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Metabolic Pathways for Natural and Unnatural Sialic Acids
… an ancient and conserved family of proteins, the glycosyltransferases, which are responsible for transferring sugars, such as sialic acid, onto glycoproteins and glycolipids. Disruptions in the sialic acid biosynthetic pathway are implicated in hereditary inclusion body myopathy (hIBM), a disease …
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A Comparative Study of Medicago truncatula Transcriptomes as They Relate to Saponin Defenses Against Insect Pests
… P450 enzymes and glycosylation is carried out by glycosyltransferases. To date, few of these cytochrome P450’s and fewer glycosyltransferases have been functionally identified. RNA was sequenced to evaluate gene transcript abundance in response to herbivory between accessions A17 and ESP105 to …
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Polyprenyl-dependent glycan assembly pathways in microbial pathogens
… since the kinase can be coupled to various glycosyltransferases to generate the complex products starting from undecaprenol and ATP. A second focus of this thesis involves the biochemical characterization of the undecaprenyl-dependent O-linked protein glycosylation pathway in Neisseria …
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Genetic Characterisation of Human ABO Blood Group Variants with a Focus on Subgroups and Hybrid Alleles
… to our knowledge of the ABO gene and encoded glycosyltransferases. Novel alleles (six Aweak, eleven Bweak, seven O) were identified containing single-point mutations. Structure/function studies explained the weakening of some B subgroup glycosyltransferases. Two new hybrid Ax alleles were …
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Structure-function relationships in monotopic phosphoglycosyl transferases
… PrenPP-sugar product is elaborated by downstream glycosyltransferases, transferred across the membrane and ultimately appended to various acceptor molecules. The PGTs initiating glycan assembly adopt diverse membrane architectures. An extensive superfamily of PGTs, elucidated in part by this …
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Glycosylation of Prostate Cancer Cell Lines and Glycosyltransferase Characterization and Inhibition
… enzymatic activities and expression levels of glycosyltransferases responsible for the syntheses of N- and O-glycans have been determined and correlated with glycan structures. We found that prostate cells are capable of synthesizing complex N-glycans and core 1 based O-glycans, and that each …
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