Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 51 for “"glycosyltransferase"”.
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Structural and functional analysis of glycosyltransferase mechanisms
… mechanisms utilized by retaining and inverting glycosyltransferase enzymes is an important stepping stone to the directed design of stereospecific inhibitor based drugs. The suitability of proposed mechanisms was probed using site directed mutagenesis of catalytically relevant residues as well …
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Synthesis and evaluation of M.tb Glycosyltransferase (MshA) inhibitors
… compete for the UDP-GlcNAc binding site of the glycosyltransferase (MshA) of M.tb. The bioactivity of the designed molecules against M.tb in cell free and whole cell assays serves as a basis for further inhibitor optimisation. Amongst the thiazolidinethione derivatives screened, compounds …
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Glycosylation of Prostate Cancer Cell Lines and Glycosyltransferase Characterization and Inhibition
… enzymatic activities and expression levels of glycosyltransferases responsible for the syntheses of N- and O-glycans have been determined and correlated with glycan structures. We found that prostate cells are capable of synthesizing complex N-glycans and core 1 based O-glycans, and that each …
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Transcriptional regulation of glycosyltransferase genes in MCF-7 human breast cancer cell line following drug treatment
… by focusing on the regulation patterns of glycosyltransferase (GT) genes in breast cancer cell line following the treatment with a large set of Food and Drug Administration (FDA) approved drugs. This is based on the understanding that aberrant glycosylation in breast cancer tumours stem …
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Methods to enable the detection of targeted AmphDI glycosyltransferase mutants that transfer GDP-C2’epimycosamine to amphoteronolide
Invasive fungal infections remain a leading cause of morbidity and mortality in the world, accounting for ~1.5 million deaths each year. Currently available treatments have important limitations, and the emergence of pathogen resistance is further weakening the clinical efficacy of most of these …
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O antigen biosynthesis in Gram-negative bacteria: Characterization of alpha1,4-glucosyltransferase WclY from Escherichia coli O117 and development of glycosyltransferase inhibitors.
… units of oligosaccharides that is assembled by glycosyltransferases. Many of these bacteria are associated with disease, and the polysaccharides are functionally important for immune responses. In light of increasing antibiotic resistance it is critical to develop new antibacterial strategies …
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Defining the tropism of Listeria monocytogenes using a comparative genomics approach
… proteins, a peptidoglycan lytic protein and a glycosyltransferase family 65 enzyme. The glycosyltransferase family 65 gene was found to be present in isolates belonging to lineage II but was absent in lineage I isolates. By creating an isogenic deletion mutant of this gene, it was found that …
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Role of VimF in Gingipain Maturation in Porphyromonas gingivalis
… unique Vim proteins including VimF, a putative glycosyltransferase. To ascertain the VimF mediated phenotype we first inactivated the vimF gene in P. gingivalis ATCC 33277 (FLL476), a less virulent fimbriated strain. We observed that the vimF-defective mutant (FLL476) showed a phenotype similar …
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Untersuchungen zur Phenalinolactonbiosynthese sowie Charakterisierung der ungewöhnlichen Glycosyltransferasen UrdGT2 und AviGT4
… sowie Charakterisierung der ungewöhnlichen Glycosyltransferasen UrdGT2 und AviGT4" von Clemens Dürr <br> <br>Im Rahmen von drei Projekten wurden Untersuchungen zur Biosynthese glycosidierter Naturstoffe durchgeführt: <br> <br>1) Phenalinolactone sind eine Gruppe antibiotisch wirksamer …
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Biosynthesis and mode of action of ribosomally synthesized and post-translationally modified antimicrobial peptides
… gene cluster led to the identification of an S-glycosyltransferase predicted to be responsible for the post-translational modification of the sublancin precursor peptide. The unprecedented glycosyltransferase was reconstituted in vitro and demonstrated remarkable substrate promiscuity for both …
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Biochemical and biophysical investigations of N-linked glycosylation pathways in archaea
… is generated by an initial retaining glycosyltransferase (AglK) and elaborated by additional glycosyltransferases (AglC and AgIA) to afford Dol-P-GlcNAc- Glc-2,3-diNAcA-ManNAc(6Thr)A. Despite the homology to other bacterial or eukaryotic OTases that exploit polyprenyl-PP-linked …
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Identification and structural bioinformatics of druggable proteins in schistosoma species
… using Hidden Markov Model. Added to this, glycosyltransferase and axonemal dynein intermediate chain schistosomal proteins were identified using in silico methods as vital proteins for the survival of the parasite in the host. The 3D structures of the AMPs and the proteins were modelled …
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Biochemistry and functional analysis of exopolysaccharide production in Lactobacillus johnsonii
… the epsE gene, that encodes the putative priming glycosyltransferase of the EPS biosynthesis, caused a huge increase in aggregation of L. johnsonii, which were shown to be related with EPS production levels. Structural analysis of the purified EPS showed that L. johnsonii could produce two types …
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Il-6/Jak1 Drives Pd-L1 Phosphorylation and Glycosylation to Promote Cancer Immune Evasion
… of endoplasmic reticulum (ER)-associated N-glycosyltransferase, STT3A, which catalyzes the glycosylation of PD-L1, contributing to its stability. A positive correlation between IL-6 and PD-L1 expression levels was observed in tumor samples from patients with hepatocellular carcinoma (HCC). …
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Studies on fucosylation in Trypanosoma brucei
… null mutant for TbFT was generated and this glycosyltransferase shown to be essential for parasite growth in vitro, with the mutant cells displaying a slightly abnormal morphology and an apparent reduction in the surface high molecular weight glycoconjugate complex. Here we also describe the …
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NOVEL STRATEGIES TO DEVELOP BETTER BRUCELLOSIS VACCINES USING BRUCELLA ABORTUS RB51 AND B. NEOTOMAE
… have shown that the wboA gene, which encodes a glycosyltransferase required for the synthesis of O-polysaccharide in Brucella, is disrupted in B. abortus RB51 by an IS711 element. Although low-levels of intra-cytoplasmic O-polysaccharide were produced when RB51 was complemented with a functional …
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Carbohydrate-Interacting Proteins from Two Nostoc (Cyanobacteria) Species
… acid sequence information from the enzymes (glycosyltransferases) that are responsible for producing the extracellular polysaccharide (EPS) of Nostoc commune DRH1, ultimately to allow the transfer of this capacity to another organism. Two artificial substrates were synthesized for use in a …
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Evolutionary and functional study of plant cell wall polysaccharide glucomannan
… that MBGT in Rosids is present in GT47A-VII (Glycosyltransferase family 47A subclade VII), whereas that in Asterids is present in GT47A-III, suggesting convergent evolution from different xyloglucan galactosyltransferases. Chapter4 addressed the question, “What is the role of CSLD (cellulose …
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Studies on the genetic basis of Pk, P and P1 blood group antigen expression
… emphasizing the genetic heterogeneity at the glycosyltransferase loci underlying these blood groups. As a result of this study, the P antigen was assigned its own blood group system, GLOB (028), by ISBT. Expression studies in the Pk-negative Namalwa cells transfected with mutated …
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Sex-specific gene expression and its effect on osteoclast maturation
… expression of gene, GALNT, which encodes a glycosyltransferase responsible for attaching N-acetylgalactosamine to serine/threonine residues. This sugar is later used as a substrate for sialylation. Our analysis found that terminal α2-3 sialylation is necessary for proper osteoclast …
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