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Showing 1 to 18 of 18 for “"glycoside hydrolase"”.
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Harnessing N-glycans as Next Generation Prebiotics
… sources in a microbiota-dependent manner. Glycoside hydrolase profiles, which are indicative of carbohydrate degradation, were significantly different between all three microbial communities regardless of treatment. Additionally, the glycoproteins had a significant impact on the glycoside …
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Biochemical characterization of five GH130-family enzymes from Caldanaerobius polysaccharolyticus ATCC BAA-17 and insights on their metabolic role and reaction mechanisms
Proteins in the glycoside hydrolase family 130 (GH130, CAZy database) have been proposed to perform the phosphorolysis of β-1,2 and β-1,4-mannosyl linkages between the mannose at the non-reducing end of substrates and mannose, glucose or N-acetylglucosamine residues, with the subsequent release of …
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The agarase system of saccharophagus degradans strain 2-40 : analysis of the agarase system and protein localization
… AgaC, AgaD and AgaE. These proteins contain glycoside hydrolase domains GH16, GH50 and GH86. S. degradans is the only organism known to collectively encode agarases with at least one of these domains. Unusual for agarases, AgaB and AgaE also contain multiple type-six carbohydrate binding …
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High-Yield Cellulosic Hydrogen Production by Cell-Free Synthetic Cascade Enzymes: Minimal Bacterial Cellulase Cocktail and Thermostable Polyphosphate Glucokinase
… bacterial cellulase mixtures containing the glycoside hydrolase family 5 Bacillus subtilis endoglucanase, family 9 Clostridium phytofermentans processive endoglucanase, and family 48 Clostridium phytofermentans cellobiohydrolase was investigated on microcrystalline cellulose (Avicel) and …
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Development of Mono-de-N-acetylated β-(1→6)-N-acetyl-D-glucosamine Substrates for Characterization of PgaB Hydrolase Activity
… PNAG. The C-terminal domain is a family 153 glycoside hydrolase whose role in vivo is not fully understood. This domain can degrade dPNAG-based biofilms, demonstrating the potential for the enzyme to be used as a therapeutic treatment for biofilm-mediated infections. It is known that the PgaB …
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Functional metagenomic analysis of carbohydrate degrading enzymes from the human gut microbiota
… hosts, was used to isolate novel genes encoding glycoside hydrolase (GH) enzymes. The study identified several active GH enzymes involved in the breakdown of dietary polysaccharides such as starch, cellulose, xylan and β-glucan, recovered from the E. coli metagenomic library. The bioinformatic …
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Enzymatic blood group conversion of human kidneys for transplantation
… on the vascular surface of human kidneys by glycoside hydrolase enzymes, resulting in the conversion of blood group A or B substrates to a core H antigen structure found in the universal donor blood group O. This work involved optimising the delivery of these enzymes to human kidneys with ex …
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Functional Genomic Examinations Of Interactions Between Common Members Of The Human Gut Microbiota
… vulgatus,</italic> upregulates its unique glycoside hydrolase activities to adapt to the presence of <italic>E. rectale,</italic> increasing its degradation of host-derived glycans that <italic>E. rectale</italic> cannot use. In contrast, <italic>E. rectale</italic> downregulates its …
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Biochemical and molecular analyses of plant cell wall degrading enzymes from the rumen bacterium Ruminococcus albus 8
… of R. albus 8 revealed five genes encoding glycoside hydrolases predicted to hydrolyze the xylose backbone and several genes encoding putative accessory enzymes that are expected to remove side chains from xylans. The genes were cloned, heterologously expressed in E. coli, and biochemically …
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"Sugar Coat" Genetic & chemical validation of <i>Aspergillus fumigatus</i> cell wall targets
… partially hydrolysed during morphogenesis by glycoside hydrolase family 18 plant-type chitinases. In contrast, exploitation of glucosamine-6-phosphate N-acetyltransferase (GNA1) an enzyme in the highly conserved UDP-GlcNAc biosynthetic pathway is an elegant means of targeting chitin synthesis …
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Modification and Translocation of the Biofilm Exopolysaccharide Poly-β(1,6)-N-acetyl-D-glucosamine
… domain displays significantly higher dPNAG hydrolase activity than PgaB, and we have identified key residues required for catalysis. The production, export, and modification machinery for PNAG biosynthesis differs in Gram-positive bacteria. Structural, functional, and mutagenesis data …
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Molecular mechanisms behind the adjustment of phototrophic light-harvesting and mixotrophic utilization of cellulosic carbon sources in Chlamydomonas reinhardtii
… revealed that it contains genes encoding glycoside hydrolases of different families, known to be involved in cellulose- and hemicellulose degradation, even though the cell wall of this alga does not contain cellulose and is solely composed of hydroxyproline-rich glycoproteins. This work …
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Effect of surface modifications on biodegradation of nanocellulose and microbial response
… rRNA genes) and anaerobic cellulose degraders (glycoside hydrolase family 48 genes, i.e., cel48) as well as high throughput amplicon sequencing of 16S rRNA genes (V4 region). A series of three studies examined: 1) the effect of nanocrystalline versus microcrystalline cellulose; 2) the effects of …
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Functional metagenomic discovery and characterisation of CAZymes by microfluidic methods
… of hit SN243. This enzyme belongs to the glycoside hydrolase (GH) family 3, a family that had no previous record of β-glucuronidase activity at the outset of this study. Detailed functional and structural characterisation provide evidence that SN243 is a genuine, efficient β-glucuronidase …
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Comparative whole genome transcriptional responses of Ruminococcus albus strain 7 and 8: a case for specialization and niche differentiation
… demonstrated that the top 5 highly expressed glycoside hydrolase (GH) families, including the versatile GH5, GH9 (Cel9B), GH10, GH11, and GH48 (Cel48A), are the primary GH enzymes employed by both strains of R. albus for the hydrolysis of plant cell wall. In addition, the co-expression of …
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Synthesis of Bulky and Polar Galactonoamidines for the Inhibition of the Human α-Galactosidase
<p>Glycosidases are amongst the most abundant enzymes in nature. This is due to their role in the degradation of carbohydrates which are the major source of carbon or earth. The absence or malfunction of glycosidases is implicated in numerous diseases such as cancers, diabetes, and lysosomal …
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Microbial glucosinolate metabolism: Characterization of Enterococcus faecalis XL1 and functional analysis of a GH4 enzyme
… phosphotransferase system (PTS) transporters and glycoside hydrolases (GHs) responsive to sinigrin, with a GH4 enzyme Ef5510, showing the strongest induction. Functional studies revealed that Ef5510 is an NAD+/Mn2+-dependent hydrolase, and the PTS-mediated uptake step represents the rate-limiting …
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Alginate and laminarin degrading enzymes from Vibrio splendidus and Vibrio breoganii
The marine bacteria V. splendidus 12B01, V. splendidus 13B01, and V. breoganii 1C10 metabolize brown seaweeds. Brown seaweeds have many advantages over terrestrial feedstocks, including fast growth and non-recalcitrant carbon feedstocks, so brown seaweeds are an attractive target for industrial …