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Showing 1 to 17 of 17 for “"glycosidases"”.
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Ginsenosides, Glycosidases and the Ginseng-Pythium Interaction
… Rb1, Rd and gypenoside XVII via extracellular glycosidases (ginsenosidases), leading to the formation of a common product, ginsenoside F2. In this thesis the potential role(s) of these extracellular ginsenosidases and the ginsenoside products they produce (ie. ginsenoside F2) in the ginseng-P. …
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Effect of glycosidases and proteases on biofilms formed on black buna-N rubber
Proteolytic enzymes and a glycolytic enzyme used in dishwashing detergents and by the starch conversion industry were examined for their ability to remove bacteria attached to black buna-N rubber. Pure culture and mixed culture biofilms of Pseudomonas fluorescens and Listeria monocytogenes were …
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Synthesis of Bulky and Polar Galactonoamidines for the Inhibition of the Human α-Galactosidase
<p>Glycosidases are amongst the most abundant enzymes in nature. This is due to their role in the degradation of carbohydrates which are the major source of carbon or earth. The absence or malfunction of glycosidases is implicated in numerous diseases such as cancers, diabetes, and lysosomal …
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Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis
… cell surface by its resistance to removal by glycosidases.
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Biochemical Studies on Enzymatic Treatment of Pulp and Paper Mill Biosludge and Polymeric Lignin
… our research on the application of proteases, glycosidases, and lignin-oxidizing enzymes for enzymatic treatments of pulp and paper mill biosludge. Biosludge pretreatment with two proteases (from Bacillus licheniformis and Aspergillus oryzae) and two glycosidases (the SCO6604 glycosidase from …
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Cell-walls of growing plant cells
… from the microfibrils by expansins or broken by glycosidases or transglycosylases. A number of researchers have presented theoretical difficulties and observations inconsistent with this model and a new hypothesis has been proposed, claiming that the cellulose – xyloglucan cross-links may act as …
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Characterisation of glycosidase enzymes of wine lactic acid bacteria
… investigated the presence and distribution of glycosidases amongst 40 isolates of Lactic Acid Bacteria (LAB) (22 of which being Oenococcus oeni and the remaining 18 equally represented by Lactobacillus and Pediococcus), as a start to investigating their potential application in winemaking. …
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Diastereoselective acylation of trans-2-substituted-cyclohexanols and glycosidase inhibition studies
… were tested as inhibitors of fungal glycosidases. Two compounds showed greater than 80% inhibition values in excess of the activity of β-D-glucosidases. More interestingly, the same compounds showed a marked enhancement of α-D-galactosidase activity by as much as 35%.</p>
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In vitro selection of deoxyribozymes for O-glycoside cleavage and for 3-nitrotyrosine modification
… synthetic preparation and structural analysis. Glycosidases and glycosyltransferases are important components of the glycobiology toolbox that facilitate manipulation of complex carbohydrate structures. Chapter 3 describes the in vitro selection of deoxyribozymes for O-glycoside cleavage. …
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Identification and characterization of a receptor for Clostridium Difficile enterotoxin
… rabbit erythrocytes with lectins and specific glycosidases revealed a high concentration of terminal α-linked galactose. Treatment of both membrane types with α-galactosidase destroyed the binding activity. The glycoprotein, calf thyroglobulin, also bound the toxin and inhibited toxin binding …
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N-linked glycosylation at position ASN98 of the ALK1 receptor protein: relevance for ALK1 function and HHT pathogenesis
… endo H and PNGase F enzymes, which represent two glycosidases able to remove N-linked oligosaccharides on proteins. Western blot analysis also revealed an identical shift in protein size (5-8 kDa) when comparing wild type ALK1 to an asparagine98-to-alanine (N98A) mutant ALK1 construct. The 5-8 kDa …
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Construction des enzymes hybrides entre la chitinase de l'orge et la chitosanase de Streptomyces SP. N174
Dans le cadre de ce projet, nous avons tenté de fusionner deux glycosyl hydrolases non homologues: la chitosanase de Streptomyces sp. N174 et la chitinase de l'orge. Ces deux protéines comptent parmi les enzymes les mieux étudiées et caractérisées au sein de leurs familles respectives. Toutes les …
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Expression and glycosylation of meprin isoforms
… of brush border membrane proteins treated with glycosidases. Nucleotide sequencing, internal peptide sequences from the β subunit, and NH₂-terminal sequence analyses (39 residues) indicated that at the amino acid sequence level, mouse β is approximately 55 % identical to mouse , and 85 % …
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The effects of different processing parameters (cold soak and percent alcohol (v/v) at dejuicing) on the concentrations of grape glycosides and glycoside fractions and glycosidase activities in selected yeast and lactic acid bacteria
Grape-derived aroma and flavor precursors exist partially as non-volatile, sugar-bound glycosides. Hydrolysis of these compounds may modify sensory attributes and potentially enhance wine quality. Cold soak (prefermentation skin contact) at two temperatures and alcohol content (%, v/v) at dejuicing …
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The effects of post-fermentation and post-bottling heat treatment on Cabernet Sauvignon (V. vinifera L.) glycosides and quantification of glycosidase activities in selected strains of Brettanomyces bruxellensis and Oenococcus oeni
Thermal processing has been used as a means of modifying the sensory aspects of wine. Cabernet Sauvignon wines were heated prior to dejuicing (3C per day from 25C to 42C) or after bottling (42C for 21 days) to determine the effects on total glycosides and glycosidic fractions. Total and phenol-free …