Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"glycohydrolase"”.
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Purification and properties of Bungarus fasciatus venom NAD glycohydrolase
The NAD glycohydrolase (NADase) from Bungarus fasciatus venom was purified over 1000-fold to electrophoretic homogeneity through a 3-step procedure which included affinity chromatography on Cibacron Blue agarose. The enzyme exhibited a broad pH profile with the optimum range between 7-8. Studies on …
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Development of poly(ADP-ribose) glycohydrolase inhibitors and tetracyclic indoles as anticancer compounds
… the target-based development of poly(ADP-ribose) glycohydrolase (PARG) inhibitors as putative anticancer compounds as well as the discovery of a class of potent anticancer tetracyclic indoles through a phenotypic screen. PARG plays a crucial role in DNA damage repair, and lack of PARG activity …
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Strategies for structural studies of poly(ADP-ribose) glycohydrolase: Towards the validation of a novel therapeutic target
… and the degrading enzyme poly(ADP-ribose) glycohydrolase (PARG). PARP-1, the first-discovered and major PARP, has been the focus of many studies aimed at clarifying the biological function of poly(ADP-ribose) (PAR). This abundant nuclear enzyme plays key roles in a variety of cellular …
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Enzymatic synthesis of defined-length poly(ADP-ribose) and the investigation of cell-permeable poly(ADP-ribose) glycohydrolase inhibitors
… BRCA-mutated cancer cells, and Poly(ADP-ribose) Glycohydrolase (PARG) inhibition is investigated as an alternate pathway to PARP inhibition in genotoxic chemotherapy. However, little is known about the exact mode of interaction between PAR and different proteins mainly due to the fact that PARP …
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Investigating the Role of PARylation in Regulating Skeletal Muscle Mass and Function in Healthy Mature Mice
… by hydrolases such as poly(ADP-ribose) glycohydrolase (PARG). This thesis characterizes the role of poly(ADP-ribose) polymerase 1 (PARP1) and PARG in the skeletal muscle of healthy mature mice under normal physiological conditions. Specifically, we validate the deletion of Parp1 and Parg …
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Chemical Tools to Monitor and Inhibit poly(ADP -Ribosyl)ation
… of the complementary enzyme poly(ADP-ribose)glycohydrolase (PARG) is available, and therefore it has been difficult to intelligently design compounds to inhibit this enzyme. To date, the known inhibitors of PARG can be predominantly grouped into the three categories of DNA intercalators, …
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Mechanistic Enzymology Of Plasmodium Falciparum Sir2, Structural And Biochemical Studies On Nicotinamidases And The Structure And Function Of Enzymes In The Purine Degradative Pathway From Klebsiella Pneumoniae
… and acetyllysine-independent NAD+ glycohydrolase. In addition, several examples of the Sir2 modulating enzyme, nicotinamidase, are characterized both structurally and biochemically. These works provided additional insights into and valuable tools for the study of Sir2-dependent …
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The Modulation of Life and Death: Small Molecule Activators and Inhibitors of Necrotic and Apoptotic Pathways
… and the inhibition of poly(ADP-ribose) glycohydrolase (PARG). Through the development of high-throughput screens, a small molecule activator of procaspase-3 (PAC-1) and an inhibitor of PARG (PIP-1) were identified. PAC-1 was shown to potently induce apoptosis in mammalian cells in a …
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The discovery of small molecule inhibitors of poly(ADP-ribose) mediated cell death
… of small-molecule inhibitors of poly(ADP-ribose) glycohydrolase (PARG), the enzyme that degrades PAR, and apoptosis inducing factor (AIF), a protein that orchestrates stage I chromatin condensation. Recognizing that the phosphate moiety, which is cell impermeable, is necessary for PARG recognition …
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The design and installation of an automatically controlled hydrocarbon fractionation unit
The NAD glycohydrolase (NADase) from Bungarus fasciatus venom was purified over 1000-fold to electrophoretic homogeneity through a 3-step procedure which included affinity chromatography on Cibacron Blue agarose. The enzyme exhibited a broad pH profile with the optimum range between 7-8. Studies on …
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Studies on pyridine nucleotide-dependent processes in Haemophilus influenzae
… respective substrates. A membrane-bound NMN glycohydrolase was demonstrated in Haemophilus influenzae. The enzyme functions with 3-acetylpyridine mononucleotide as a substrate, and is inhibited effectively by 3-aminopyridine mononucleotide. The possible involvement of this enzyme in the …
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Characterization Of Streptolysin O As The Translocator For The Cytolysin-Mediated Translocation Process In Streptococcus Pyogenes
… <underline>N</underline>AD<super>+</super>-glycohydrolase: SPN) effector into the cytosol of eukaryotic host cells. Although, the fundamentals of this protein translocation process are established the details of the mechanism remain elusive. The current studies illustrate that the …
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Comprehensive Investigation into the Molecular Mechanisms Driving the Emergence of emm4 Group A Streptococcus
… of the genes encoding the potent toxins NAD-glycohydrolase and streptolysin O (Nga/Slo) and upregulation of the <em>msrAB</em> operon, which encodes proteins involved in defense against extracellular oxidative stress. Our findings are in accord with recent surveillance studies, which found a …
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Investigating the effects of calreticulin and beta-galactosidase on microglial functions and neuronal loss
… in the brain might be beneficial for AD. Glycohydrolase enzymes, including neuraminidase 1 (Neu1) and β-galactosidase, play a fundamental role in the degradation of glycoproteins and glycolipids, to maintain cellular constituent turnover and glycosylation homeostasis. Neu1 hydrolyses …
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The chemical and enzymatic synthesis of poly(ADP-ribose)
Poly(ADP-ribose) synthesis and degradation are important cellular processes associated with the DNA damage response and many other pathways. Traditionally, these processes have been challenging to study due to the heterogeneity associated with the synthesis of poly(ADP-ribose). Discussed here are …