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Showing 1 to 20 of 32 for “"glycoconjugate"”.
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Optimization of Burkholderia Glycoconjugate Vaccines: A Reverse Vaccinology Approach
… development. Previous studies have shown that glycoconjugate vaccines can provide enhanced protection against lethal B. pseudomallei challenge. However, the limited pool of Burkholderia antigens hinders continued optimization of these vaccines. In this study, we used a reverse vaccinology …
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Optimization of Burkholderia Glycoconjugate Vaccines: A Reverse Vaccinology Approach
… development. Previous studies have shown that glycoconjugate vaccines can provide enhanced protection against lethal B. pseudomallei challenge. However, the limited pool of Burkholderia antigens hinders continued optimization of these vaccines. In this study, we used a reverse vaccinology …
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Chemical tools to probe carbohydrate recognition and glycoconjugate biosynthesis
In eukaryotes the assembly of complex glycoconjugate structures takes place in the endoplasmic reticulum (ER) and Golgi apparatus, where a number of specific membrane-bound glycosyltransferases transfer sugar moiety from soluble or lipid linked activated sugar nucleotides to variety of acceptors …
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Membrane protein conformational dynamics and ligand-binding interactions in bacterial glycoconjugate biosynthesis
… phosphate acceptor in the initiating step of glycoconjugate biosynthesis in prokaryotes. The pipeline is employed to confirm the role of SmPGT conformational dynamics in substrate binding and informs the design of non-hydrolyzable substratemimetic inhibitors. Chapter 5 further sets the stage …
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Progress in the synthesis of stabilized glycoconjugate vaccine candidates against Neisseria meningitides group A
Meningitis is the inflammation of the lining membranes of human brains and spinal cord. It is a deadly disease that has claimed millions of lives throughout the world in particular in developing countries. Neisseria meningitidis serogroup A is among the leading causative agents of meningitis in …
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Insights into the substrate specificities, interactions and regulatory mechanisms of bacterial glycoconjugate biosynthesis enzymes
… that initiate the assembly of prokaryotic glycoconjugates essential for bacterial survival and proliferation. MonoPGTs belong to an expansive superfamily with a diverse and richly annotated sequence space; however, limited biochemical characterization of this enzyme superfamily has been …
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Molecular mechanism of Abeta recognition and neuroprotection by the glycoconjugate beta-sheet-breaker peptide Ac-LPFFD-Th
Inhibition of amyloid formation may represent a promising therapeutic approach for the treatment of neurodegenerative diseases. To this regard, peptide-based inhibitors of Abeta aggregation have been widely investigated with a particular emphasis to those derived from original amyloid sequences. …
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Towards the Synthesis of the Unusual Monosaccharides Found in the Shigella sonnei O-Antigen and Analysis of Shigella flexneri 2a Glycoconjugate Vaccine Samples
… a spectroscopic analysis of Shigella flexneri 2a glycoconjugate and glycopeptide samples. A full set of nuclear magnetic resonance (NMR) spectra were recorded and analysed, resulting in the unambiguous determination of the structure and integrity of the O-antigen saccharide component.
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The application of physicochemical methods for the analysis of small and complex pharmaceutical drugs
… and further extended to analysis of complex glycoconjugate vaccines.
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Synthesis of Novel Calix[4]arene Based Dendrimers and their Biological Applications
… 2 describes the development of calixarene glycoconjugates using a wide range of approaches. The synthesis of calixarene glycoconjugates through click chemistry is reported and the development of the first multicalixarene glycoconjugate is discussed. Chapter 3 reviews the development of …
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The Effects of Retinoids on Regenerating Axolotl Limbs
… have been shown to alter adhesivity and glycoconjugate composition of cell surfaces in cultured avian and mammalian cells. These two observations suggest a potential approach to a cellular and molecular analysis of positional value in regenerating limbs.
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Structure-function relationships in monotopic phosphoglycosyl transferases
… modification takes a diversity of forms, many glycoconjugate biosynthesis pathway across domains of life follows a common logic. Glycan assembly is initiated by a phosphoglycosyl transferase (PGT) that transfers a phosphosugar from a nucleotide donor to a polyprenol phosphate (PrenP) chain …
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Chemical synthesis of glycosylated oligoribonucleotides for siRNA development
… an amino modified oligoribonucleotide to form a glycoconjugate under slightly basic conditions. The effect of glycosylation on the stability of RNA molecules was evaluated on two glycoconjugates, monomannosyl UlO-mer and dimannosyl UlO-mer. In the synthesis of aromatic fluorescent ribosides, …
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Therapeutics discovery via glycotechnology : the development of protein engineered enzymes for the study of galactosaminoglycan neuromedicine
… This library of enzymatic tools will reveal glycoconjugate structure-function motifs, allowed for by structural elucidation of glycan species using coupled bioinformatics/analytical chemistry techniques centered on mass spectrometry, nuclear magnetic resonance, and capillary electrophoresis.
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NMR characterisation of group b streptococcus capsular polysaccharide repeating units
… study attempts to aid the development of GBS glycoconjugate vaccines by fully characterising the repeating units of the six most common GBS serotypes (Ia, Ib, II - V) by NMR recorded at a higher temperature of 343 K to serve as a database of reference GBS NMR spectra and chemical shift …
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Studies on fucosylation in Trypanosoma brucei
… reduction in the surface high molecular weight glycoconjugate complex. Here we also describe the various experimental approaches that were used to try to identify the fucosylated glycocojugates in T. brucei. Lastly, to better understand the biosynthesis of GDP-Mannose, the starting metabolite …
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Identification and Characterization of Novel Melioidosis Vaccine Candidates
… 1 (Hcp1) with a capsular polysaccharide-based glycoconjugate (CPS-CRM197) could be used to immunize C57BL/6 mice against a lethal inhalation challenge with B. pseudomallei. Additionally, we showed that 100% of the vaccinated mice survived for 35 days post-challenge and that 70% of those mice …
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Evaluation of a gold nanoparticle platform as highly immunogenic and protective therapy against Burkholderia mallei, B. pseudomallei, and Enterohemorrhagic Escherichia coli O157:H7
… both glanders and melioidosis. In this study, glycoconjugates coupled to gold nanoparticles (AuNPs) were tested for their protective properties in clinically relevant infection models. The flexible NP platform allow us to incorporate novel antigens identified previously by a reverse vaccinology …
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Dechalcogenative Allylic Selenosulfide And Disulfide Rearrangements For Cysteine Modification And Glycoligation
… The protecting group-free synthesis of a glycoconjugate was accomplished using this methodology.</p> <p>In chapter four, extending the concept of this novel metal mediated allylic desulfurative rearrangement as a chemoselective ligation technique, for the synthesis of complex …
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Synthesis Of Functionalized Gpi Anchors And Related Glycoconjugates
… to which complex glycans are attached. This glycoconjugate exists in two distinct functional forms, including lymphocyte and sperm CD52, which are involved in the human immune and reproductive systems, respectively. The contrasting biological functions arise from structural differences in …
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