Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"glutathione S-transferase (GST)"”.
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Influence of molecular and genetic factors of the organism΄s antioxidative system on cervical carcinogenesis and the role of these factors in treatment of locally advanced cervical cancer /
… - to assess the importance of molecular (reduced glutathione (GSH) and glutathione S-transferase (GST)) and genetic (glutathione S-transferase (GST) genes polymorphism) factors determining the activity of antioxidative system for development of cervical cancer and evaluate the role of these …
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The Mechanism of Cyanohydroxybutene Induced Glutathione Elevation: The Role of Gamma-Glutamylcysteine Synthetase
… mg/kg po) causes a 2- and 7-fold elevation in glutathione (GSH) in liver and pancreas, respectively. The mechanism of elevation is currently unknown. In an attempt to identify the mechanism of this GSH induction, the effect of CHB on hepatic and pancreatic $\gamma$-glutamylcysteine synthetase …
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Characterization of Smooth Muscle Myosin Light Chain Kinase Using Crystallization and Transient Kinetics
… actin-binding domain of MLCK (called N1-75) with glutathione S-transferase (GST) was attempted in order to provide such a model. Polyethylene glycol and ammonium sulfate, within a pH range of 6.5 to 8.0, seemed to be the most promising precipitants to induce crystallization. Observation of …
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The development of a model system and high throughput assay for the study of interactions between zinc finger proteins and DNA
… was altered and the protein synthesised as a Glutathione S-Transferase (GST) fusion product. A successful assay was developed using the GST protein and Scintillation Proximity Assay technology (Amersham Pharmacia Biotech). The scintillation proximity assay is a dynamic assay that allows the …
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Breast Cancer Inhibition by Grape Constituents
… activity of the phase II metabolizing enzyme, glutathione S-transferase. Based on in vivo research, we evaluated grape color extract (GCE) and two select anthocyanins for their capacity to inhibit benzo[a]pyrene (BP)-induced DNA adduct formation in MCF-10F cells. GCE and each anthocyanins …
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Biochemical and molecular basis for metabolism-based atrazine resistance in Amaranthus tuberculatus
… s-triazine herbicides is the result of increased glutathione S-transferase (GST) activity in atrazine-tolerant crops such as maize and grain sorghum (Sorghum bicolor), or in atrazine-tolerant weeds such as Panicum spp. In previous research, two atrazine-resistant populations of tall waterhemp …
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Factors influencing arbovirus transmission: vector competence and the effects of virus infection on repellent response, oxidative stress, and glutathione-S-transferase activity
… ZIKV and LACV infection on oxidative stress and glutathione-S-transferase (GST) activity in Aedes albopictus. Virus infection had no effect on oxidative stress, but GST activity was significantly different for mosquitoes 3-days post-exposure. We found that oxidative stress levels and GST activity …
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Mechanisms of Human LARP6 Nuclear Export
… recombinantly expressed putative NES motifs as glutathione S-transferase (GST) fusion proteins for use in in vitro CRM1 pulldown assays. We identified three additional putative NES using LocNES, a more recent and robust NES identification algorithm. We have also designed NES deletion mutants, …
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Development And Optimization Of The First High Throughput In Vitro Fret Assay To Characterize The Saccharomyces Cerevisiae Gpi-T
… a plasmid that expresses Gpi8 with an appended glutathione-S-transferase (GST) domain. Peptide substrates for GPI-T were synthesized and modified to contain a pair of chromophores suitable for the development of a fluorescence resonance energy transfer (FRET) assay. GPI-T activity was observed …
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Cysteine arylation
… and biological foundation of life. The longstanding goals of chemical biology are to understand the structure and function of proteins and to use these biomolecules for applications in chemistry, biology, medicine, and material science. To achieve such goals, highly efficient, selective, …
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Characterization of resistance to halogenated aromatic hydrocarbons in a population of Fundulus heteroclitus from a marine superfund site
… was not higher in NBH fish than in SC fish. Glutathione S-transferase GST and UDP-glucuronosyltransferase UGT activities were higher in NBH fish than in SC fish, but only when fish were collected during different seasons. GST activity was higher in the intestines ofNBH fish than in any other …
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The effect of a dietary phase 2 protein inducer on inflammatory parameters in blood and liver of spontaneously hypertensive stroke prone rats
… an increase in plasma protein thiols and blood glutathione (GSH)), 3. Acute phase proteins (a decreased APR is reflected by an increase in plasma albumin and a decrease in ceruloplasmin), 4. Activity of phase 2 enzymes (increased phase 2 enzyme induction results in higher activities of liver …
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Development and Application of a Fluorescence Anisotropy Microplate Assay for Analyzing the Interaction of Steroid Hormone Receptors With DNA and Coactivators
… steroid receptors. Finally, we used a modified Glutathione S-transferase (GST) pull down assay to investigate the role of the ERE in P160 coactivator recruitment. In the absence of the cERE, addition of whole cell extract caused a 3-4 fold reduction in ER-coactivator interaction, compared to …
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Improving the Health Benefit of Broccoli by Maximizing Sulforaphane Formation: A Role for Epithiospecifier Protein
… enzymes quinone reductase (QR) and glutathione S-transferase (GST). Mild heat treatment was found to dramatically increase the formation of sulforaphane from broccoli while decreasing the formation of the alternative nitrite product. A heat sensitive epithiospecifier protein (ESP) …
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Site-selective modification of cysteine residues
… between cysteine and lysine residues. A glutathione S-transferase (GST) catalyzed site-selective macrocyclization reaction for peptides up to 40 amino acids in length is also reported.
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Differential tolerance of corn hybrids to metolachlor and its regulation by the safener benoxacor
… in plants is mediated via conjugation with glutathione (GSH). GSH levels from 1.8 to 2.4 µmol/g fresh weight were determined for the eleven corn hybrids tested. There was no correlation between GSH content and herbicide tolerance. The monooxygenase inhibitor piperonyl butoxide (PBO) acted …
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Palladium uptake and its effect on behavioural and molecular markers in the freshwater shrimp Gammarus pulex
… bromide (MTT) reduction; reduced glutathione (GSH); glutathione-S-transferase (GST); thiobarbituric acid reactive substances (TBARS); heat shock proteins (HSPs) and protein phosphorylation) endpoints. Pd caused a significant reduction in the survival of G. pulex with a 96 h LC50 of …
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Point mutational examination of eukaryotic initiation factor 4E (eIF4E), inclusion bodies analysis, and Barley Yellow Dwarf Virus (BYDV) exonuclease resistant RNA structure mapping and prediction
… chosen method of eIF4E purification involving a glutathione S-transferase (GST) tag repeatedly resulted GST-eIF4E inclusion bodies, which limited the ability to purify mutant eIF4E for binding studies. Therefore a large portion of this work is concerned with attempts to solubilize the GST-eIF4E …
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In vitro studies on the cellular mechanisms of resistance to cytotoxic compounds with special reference to multidrug resistance and cancer chemotherapy
… cell line. No significant difference in the glutathione content was observed although a significant increase in glutathione-S-transferase (GST) activity was detected in the EMT-6/R cell line. Although resistance was selected using doxorubicin, the EMT-6/R cell line also displayed …
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The potential application of rapeseed pomace extracts in the prevention and treatment of neurodegenerative diseases.
… on the activation of the antioxidant gene glutathione-s-transferase (gst-4) in C. elegans. Overall, the RSP extract showed very positive in vitro characteristics and valuable in vivo effects in 4 disease C. elegans models, thus warranting further detailed studies on the use of RSP extract …
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