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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"glutamine synthetase"”.
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Glutamine synthetase in Bacteroides fragilis
… assimilated in B. fragilis through the action of glutamine synthetase (GS). Glutamine is vital to nitrogen metabolism as it serves as a precursor to many secondary metabolites. GS enzymes are, therefore, vital to the growth of the organism and many prokaryotes are known to possess two or more …
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Regulation of clostridium acetobutylicum glutamine synthetase glnA gene
… butanol and ethanol. The C. acetobutylicum glutamine synthetase (GS) glnA gene region originally cloned in Escherichia coli, is characterized by the glnA structural gene, the upstream promoter sequences P₁ and P₂, a downstream DNA sequence complementary to the 5'end of the glnA gene and the …
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Genetic and biochemical studies of Vibrio alginolyticus glutamine synthetase
… the positive selection vector pEcoR251. A glutamine synthetase (GS) gene, glnA was identified by complementation of the glnA deletion in E. coli ET8051 glnA, glnL, glnG deletion strain. The glnA region of V. alginolyticus was cloned on a 5.7 kb insert in pRM210.
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Crystal structure of the Large Type III Glutamine Synthetase from Bacteroides Fragilis
Glutamine synthetases are one of the most ancient functioning enzymes in existence and these large oligomeric complexes are found in all extant forms of life where they play a critical role in nitrogen metabolism. Over the past five decades, extensive biochemical studies together with structural …
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Three-dimensional structure of a type III glutamine synthetase by single particle reconstruction
… first structural investigation of any type III glutamine synthetase (GS). The GS, GlnA, from the medically important opportunistic human pathogen Bacteroides fragilis was studied with a view to better understanding its structure/functioning in relation to the extensively characterised GSIs. …
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Soluble expression of plasmodium falciparum glutamine synthetase and three-dimensional structure by single particle reconstruction
… and therefore inhibiting the transportation of glutamine from the host, has long been considered a potential strategy for combating the spread of infection. The recently sequenced Plasmodium falciparum genome has however shown that pathways for independent survival are also conserved. Therefore, …
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Elucidation of the reaction mechanisms involved in the catalysis mediated by glutamine synthetase in Escherichia coli
Structural and molecular dynamics analysis of the glutamine synthetase from E. coli indicates that a possible mechanism by which the adenylylation/deadenylylation of the enzyme affects the enzyme specificity for either MgATP or Mn2ATP and NH4+ or NH3, is by switching between two putative serine …
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Use of methionine sulfoximine to dissect the role of glutamine synthetase and glutamine in progression of acute liver failure
… a well characterized irreversible inhibitor of glutamine synthetase (GS) enzyme. Glutamine is synthesized by GS enzyme and it is the most abundant amino acid in the body. Glutamine is required by immune cells for generation, propagation and maintenance of an immune response. </p> <p>To induce …
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Interaction between positive- and negative- regulatory elements in the first intron controls transcription of the glutamine synthetase gene during adipocyte differentiation
… of transcription, the regulatory region of the glutamine synthetase (GS) gene was investigated using both functional and binding analyses. GS expression increases more than 100-fold during differentiation of 3T3-L1 cells into adipocytes. Recently, Hadden et al. (Nucleic Acid Research, …
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Characterisation of the effect of stress on nitrogen metabolism in the commercially important agarophyte, Gracilaria gracilis
… metabolic enzymes, nitrate reductase and glutamine synthetase. Long term culture of G. gracilis in nitrogen replete and lacking conditions indicated that nutrient limitation causes a reduction in intracellular nitrogen and nitrogen protein stores such as phycoerythrin. When various sources …
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Analysis of the ammonium assimilation pathways of the human colonic bacterium, bacteroides thetaiotaomicron
… glutamate dehydrogenase (GDH) pathway and the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway. To gain insight into the genomic features underlying ammonium uptake and assimilation in this bacterium, comparative transcriptomic analysis using RNA-Seq was employed on cultures growing …
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Efficacy of glufosinate and weed control in transformed corn and soybean
… is a postemergence herbicide that inhibits the glutamine synthetase enzyme in plants, causing an accumulation of ammonia, inhibition of photosynthesis, and ultimately plant death. The use of this herbicide has been limited to no-tillage prior to planting or in noncrop areas. Recent developments …
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Functional analysis of the GlnK1 protein of Methanosarcina mazei strain Gö1: Aspects of nitrogen regulation
… jedoch, dass das GlnK1-Protein mit der Glutamin-Synthetase (GlnA) interagiert und hierdurch deren Enzymaktivität inhibiert. Ein aktivierender Effekt auf die GlnA Enzymaktivität wurde hingegen bei Anwesenheit von 2-Oxoglutarat beobachtet, welches den internen Stickstoffstatus wiederspiegelt. Aus …
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The cloning and characterization of a Butyrivibrio fibrisolvens H17c glnA regulatory element in E. coli
… in the regulation of the B.fibrisolvens type III glutamine synthetase (GS), which is a key enzyme involved in ammonia assimilation in rumen bacteria. An existing B. fibrisolvens gene bank, as well as the B. fibrisolvens glnA gene cloned onto a low 11 copy number plasmid, were used to generate a …
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An investigation of the pathway of nitrogen incorporation into the leaf and root metabolism of Helianthus annuus L
… major routing of newly reduced nitrogen to glutamine. Of the other major soluble amino compounds, alanine, aspartate, serine, and glycine were found to be important in the primary assimilation of newly reduced nitrogen. After pre-treatment with feeding solutions containing 5mM methionine …
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Assessing the Effects of Temperature, Feeding Status, and the Microbiome on the Maximal Activities of Several Enzymes Important to Energy Production and Ammonia Detoxification in the Intestines of Teleost Fish
… (NKA), citrate synthase, pyruvate kinase, and glutamine synthetase (GS). Increased NKA activity following a meal suggested an increase in ATP demand, and amplified GS activity likely assists in enhanced NH3-detoxification processes in the intestinal tract. There was a strong effect of host …
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SYNTHESIS OF N<sub>B</sub>-ARYL-ASPARTAMIDES, N<sub>a</sub>-ARYLAMIDE-ASPARTATES, AND HYDROXY-L-PROLINE DERIVATIVES AS INHIBITORS OF AMINO ACID TRANSPORT FOR EVALUATING THE GLUTAMINE / GLUTAMATE CYCLE
The glutamate / glutamine cycle is most traditionally described as the recycling of L-glutamate and L-glutamine via a concerted effort by a variety of cell membrane spanning amino acid transporters and amino acid converting enzymes, glutaminase and glutamine synthetase. This work details the design …
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SYNTHESIS OF N<sub>B</sub>-ARYL-ASPARTAMIDES, N<sub>a</sub>-ARYLAMIDE-ASPARTATES, AND HYDROXY-L-PROLINE DERIVATIVES AS INHIBITORS OF AMINO ACID TRANSPORT FOR EVALUATING THE GLUTAMINE / GLUTAMATE CYCLE
The glutamate / glutamine cycle is most traditionally described as the recycling of L-glutamate and L-glutamine via a concerted effort by a variety of cell membrane spanning amino acid transporters and amino acid converting enzymes, glutaminase and glutamine synthetase. This work details the design …
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Trimethylation Enhancement using Diazomethane (TrEnDi) Enhances Mass Spectrometry-Based Analysis of Glufosinate and 3-(Methylphosphinico)propionic Acid in Complex Matrices
… commonly used herbicide worldwide; it inhibits glutamine synthetase, which results in increased ammonia levels in plants and mammals. Due to their high polarity, low volatility, small size and lack of chromophores and fluorophores, glufosinate and its breakdown product 3-(methylphosphinico) …
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