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Showing 1 to 20 of 300 for “"glutamine"”.
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Glutamine synthetase in Bacteroides fragilis
… assimilated in B. fragilis through the action of glutamine synthetase (GS). Glutamine is vital to nitrogen metabolism as it serves as a precursor to many secondary metabolites. GS enzymes are, therefore, vital to the growth of the organism and many prokaryotes are known to possess two or more …
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Glutamine Phosphoribosyl Pyrophosphate Amidotransferase From Bacillus Subtilis
Made available in DSpace on 2014-12-14T04:27:47Z (GMT). No. of bitstreams: 1 7913663.pdf: 4656709 bytes, checksum: 461aee54ab59f2ed0c2e40bce3810c47 (MD5) Previous issue date: 1978
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Use of methionine sulfoximine to dissect the role of glutamine synthetase and glutamine in progression of acute liver failure
… a well characterized irreversible inhibitor of glutamine synthetase (GS) enzyme. Glutamine is synthesized by GS enzyme and it is the most abundant amino acid in the body. Glutamine is required by immune cells for generation, propagation and maintenance of an immune response. </p> <p>To induce …
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Regulation of clostridium acetobutylicum glutamine synthetase glnA gene
… butanol and ethanol. The C. acetobutylicum glutamine synthetase (GS) glnA gene region originally cloned in Escherichia coli, is characterized by the glnA structural gene, the upstream promoter sequences P₁ and P₂, a downstream DNA sequence complementary to the 5'end of the glnA gene and the …
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Myc influences glutamine metabolism to induce autophagy in tumorigenesis
… cells. The direct connection between Myc, glutamine metabolism and autophagy and their role in competitive events between cancerous cells and wild type cells have not been clearly explained; therefore, the main purpose of this project is to determine a plausible link between Myc and …
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Inactivation of Glutamine Phosphoribosyl Pyrophosphate Amidotransferase in Bacillus Subtilis
Made available in DSpace on 2014-12-14T04:27:33Z (GMT). No. of bitstreams: 1 7524417.pdf: 4422518 bytes, checksum: 2fcd34aaf1e4447b579382dfd2fa4456 (MD5) Previous issue date: 1975
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Genetic and biochemical studies of Vibrio alginolyticus glutamine synthetase
… the positive selection vector pEcoR251. A glutamine synthetase (GS) gene, glnA was identified by complementation of the glnA deletion in E. coli ET8051 glnA, glnL, glnG deletion strain. The glnA region of V. alginolyticus was cloned on a 5.7 kb insert in pRM210.
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Targeting Glutamine Metabolism in Kidney Development and Polycystic Kidney Disease
… Lkb1 mutant cells and embryonic kidneys require glutamine for growth while wild-type cells and kidneys did not. Subsequent studies demonstrated that Pkd1 embryonic kidneys phenocopied Lkb1 mutant kidneys in respect to their reliance on glutamine for growth. Further investigation into defining …
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Glutamine Antagonism and Its Utlity [sic] as a Therapeutic Modality in Cancer
Glutamine metabolism is important in cancer as it fuels the TCA cycle, plays a role in redox homeostasis, and contributes to the production of nucleotides, amino acids, and lipids for survival, making glutamine metabolism a promising target in cancer therapy. The work outlined in this dissertation …
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Crystal structure of the Large Type III Glutamine Synthetase from Bacteroides Fragilis
Glutamine synthetases are one of the most ancient functioning enzymes in existence and these large oligomeric complexes are found in all extant forms of life where they play a critical role in nitrogen metabolism. Over the past five decades, extensive biochemical studies together with structural …
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Glutamine Phosphoribosyl Pyrophosphate Amidotransferase From Bacillus Subtilis: Iron-Sulfur Biochemistry and Enzymology
The iron-sulfur prosthetic group of glutamine PRPP amidotransferase was found to be a {4Fe-4S} cluster. In the native enzyme, the cluster exists in the diamagnetic +2 redox state. Treatment of the cluster with oxidants resulted either in no reaction or in oxidative dissolution of the cluster, i.e., …
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Three-dimensional structure of a type III glutamine synthetase by single particle reconstruction
… first structural investigation of any type III glutamine synthetase (GS). The GS, GlnA, from the medically important opportunistic human pathogen Bacteroides fragilis was studied with a view to better understanding its structure/functioning in relation to the extensively characterised GSIs. …
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Soluble expression of plasmodium falciparum glutamine synthetase and three-dimensional structure by single particle reconstruction
… and therefore inhibiting the transportation of glutamine from the host, has long been considered a potential strategy for combating the spread of infection. The recently sequenced Plasmodium falciparum genome has however shown that pathways for independent survival are also conserved. Therefore, …
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A metabolic perturbation by U0126 identifies a role for glutamine in resveratrol-induced cell death
… of mitochondrial metabolism and focused on how glutamine supplementation of citric acid cycle intermediate a-ketoglutarate may be involved. Suppression of the conversion of glutamate to a-ketoglutarate with the transaminase inhibitors cycloserine and amino oxyacetate rescued C4-2 cells from …
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Investigating the interdependence of human BH3-only protein Noxa and glutamine in activated CD8+ T cells.
… glycolysis, utilizing alternative fuels such as glutamine or fatty acids for energy production. Here we have investigated the role of glutamine in regulating Noxa expression and the role of Noxa in the activation-induced metabolic switch in human CD8+ T cells. The absence of glutamine during T …
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Effect of glutamine supplementation combined with resistance training on muscular performance, body composition and protein degradation
The purpose was to assess the effect of oral glutamine supplementation combined with resistance training in young adults. Thirty-one subjects, aged 18- 24, were randomized (double blind) to receive glutamine (0.9g/kg lean tissue mass; n=17) or placebo (0.9g/kg lean tissue mass; n=14) during 6 weeks …
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Chemistry and Regulation of the Oxygen-Dependent Inactivation of Glutamine Phosphoribosyl Pyrophosphate Amidotransferase From Bacillus Subtilis
… inactivation of Bacillus subtilis glutamine P-Rib-PP amidotransferase in vitro and in vivo. A detailed description of the physical and chemical changes occurring in purified amidotransferase has been obtained. Inactivation of the enzyme by oxygen results in oxidation of the …
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Elucidation of the reaction mechanisms involved in the catalysis mediated by glutamine synthetase in Escherichia coli
Structural and molecular dynamics analysis of the glutamine synthetase from E. coli indicates that a possible mechanism by which the adenylylation/deadenylylation of the enzyme affects the enzyme specificity for either MgATP or Mn2ATP and NH4+ or NH3, is by switching between two putative serine …
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The Role of Glutamine and Mevalonate Pathway In Affecting The Stemness and Viability of Glioblastoma Stem Cells
… were also observed. Furthermore, we found that glutamine uptake was higher in GBM stem cells compared with FBS induced cells. Additionally, treatment of the cells with Compound 968, a glutaminase inhibitor, depleted the cells from the CD133 marker, slowed proliferation and limited growth on soft …
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