Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"glutamatergic neurons"”.
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Prdm13, a Direct Downstream Target of Ptf1a, Regulates the Balance of Gabaergic Neurons versus Glutamatergic Neurons in the Dorsal Spinal Cord
… of inhibitory versus excitatory (E/I balance) neurons. The combination of basic helix-loop-helix (bHLH) and homeodomain (HD) transcription factors is essential to generate the E/I balance required for correct functioning of neural networks. During dorsal spinal cord development, the bHLH factor …
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The Role of PTF1A in Spinal Cord Development
… that PTF1a is present in precursors to GABAergic neurons in both the cerebellum and the spinal cord dorsal horn. A null mutation in Ptf1a reveals its requirement for the dorsal horn GABAergic neurons. Specifically, PTF1a is required for the generation of early born (dI4, E10.5) and late born …
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Development of selective high-affinity fluorescent sensors for imaging neurotransmitters and application in cells and tissue
… Also, the sensor can label glutamate in cultured glutamatergic neurons, astrocytes and cortex brain.
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Cellular Phenotypes Associated with NRXN1 Mutations in Autism; an iPSC Study
… is needed. In this thesis I have used induced neurons derived from induced pluripotent stem cells (iPSC) using the OPTi-OX system to study the impact of NRXN1 mutations on excitatory glutamatergic neurons. I specifically study the molecular, morphological and functional changes caused by 1) …
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The Role of the Circadian Clock in the Paraventricular Nucleus of the Hypothalamus in Diurnal Energy Balance
… rhythms that maintain energy balance. While glutamatergic neurons undergo widespread changes in gene expression over the diurnal cycle, astrocytes and oligodendrocytes also show pronounced, diurnal changes in gene expression. Finally, restoring the circulating oxytocin (OXT) peak in BMAL1-PVN …
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Axon Development and Synapse Formation in Olfactory Sensory Neurons
… and differentiate into olfactory sensory neurons (OSNs) that project their axons to the olfactory bulb (OB) where they establish connections to the central nervous system. The olfactory epithelium is therefore an attractive model for the study of axonal growth and synapse formation. The …
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Investigating Cell-Type-Specific Vulnerability in Mitochondrial Disease Using Stem Cell-Derived Neuronal Models
… vulnerability of GABAergic inhibitory interneurons to mutations in the mitochondrial DNA polymerase catalytic subunit POLG, while the second focused on the susceptibility of spinal motor neurons to loss of function of the mitochondrial translation release factor MTRFR. Both models …
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Optogenetic Interrogation of Primary Visual Cortex and Its Impact On Neural Coding and Behavior
… activity of large populations of interconnected neurons, but while the responses of individual neurons to a variety of sensory stimuli have been well-characterized, how <em>populations</em> of such neurons organize their activity to create our sensory perceptions is almost entirely unknown. To …
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Investigating circuit and spatial heterogeneity of human brain tumors with patient-derived organoid models
… present in the tumor microenvironment, such as neurons, contribute to glioma progression. This dissertation represents a multi-pronged effort to unravel GBM heterogeneity by leveraging patient-derived GBM organoids (GBOs), a 3-dimensional model inspired by human brain organoids. In the first …
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The Role of Glutamate in the Medial Prefrontal Cortex in the Acquistion and Expression of Conditioned Approach
… experiments were conducted to assess the role of glutamatergic stimulation in the dorsal and ventral regions of the medial prefrontal cortex (mPFC) in both the acquisition and expression of reward-related learning using a Pavlovian conditioning paradigm, the conditioned-approach paradigm. Rats …
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Neural Mechanisms that Control an Innate Foraging Behavior in Caenorhabditis Elegans
… In each module, chemosensory or mechanosensory glutamatergic neurons that detect food-related cues trigger local search by inhibiting separate integrating neurons through a metabotropic glutamate receptor, MGL-1. The chemosensory and mechanosensory modules are separate and redundant, as …
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MODELING AND MODULATING MYELOID CELL-MEDIATED NEUROINFLAMMATION FOLLOWING HUMAN IMMUNODEFICIENCY VIRUS-1 INFECTION
… pluripotent stem cell (iPSC)-derived (i3) neurons to identify the effects of these insults in a model of pure glutamatergic neurons. We found i3 neurons resisted death following exposure to HIV-infected myeloid cell supernatants and integrase inhibitors, contrasting reports in primary …
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Regulation of ageing by electrical synapses in the Nematode Caenorhabditis elegans
… of cells to modulate longevity. Its removal from glutamatergic neurons led to an increase in lifespan, whereas I found no effects of unc-9 knock-down in GABAergic, dopaminergic or cholinergic neurons or muscle. Interestingly, my RNAi results point to a potential neural circuit mostly consisting of …
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Biohybrid Neural Interfaces: Exploring the Role of Regional Astroglia in Neuronal Integration and Functionality
… to neural implantation by incorporating living neurons and traditional neural implants. This creates a dynamic cellular environment that allows for more natural interactions with host neural tissues. Given that BNIs incorporate living neurons, their functionality is potentially impacted either …
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Genetic dissection of circuits underlying the modular structure of the Superior Colliculus
… is exclusively expressed in a subpopulation of glutamatergic neurons in the motor domain of the SC. Strikingly, this population of neurons displays a non-homogenous distribution within the motor layer of the SC, being organised in clusters along the mediolateral and anteroposterior axis. We …
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Developing High Purity Embryonic Stem Cell-Derived V2a Interneurons for In Vitro Investigation and Transplantation Following Spinal Cord Injury
… the major obstacle to manipulating specific interneurons for therapy: an inability to obtain large, enriched interneuron subpopulations for drug screening and transplantation. We focused on the generation of V2a interneurons, which normally coordinate a wide range of locomotor tasks in both the …
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Direct generation of region-specific induced neural Precursors from adult human fibroblasts using non-viral and viral methods
… neural precursor cells, and subsequently mature neurons and glia, by the introduction of the defined neural transcriptional factors SOX2 and PAX6. However, the understanding of the underlying gene changes that occur during reprogramming was limited, as gene characterisation in direct …
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CXCL12/CXCR4 signaling in mesocorticolimbic reward pathways: relevance to cocaine reinforcement and relapse
… (CNS), activation of CXCR4 on dopaminergic neurons and astrocytes initiate cascade of events that leads to DA and GLU release and influence synaptic transmission. In vivo, intracerebroventricular (ICV) CXCL12 has been shown to potentiate cocaine-induced locomotor activity. Based on these …
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Using yeast to study neurodegenerative diseases : amyloid formation as a protective mechanism and a new Alzheimer's disease model
… 1-42 toxicity in a neuronal setting, C. elegans glutamatergic neurons. Finally, we showed that PICALM could protect primary rat cortical neuron cultures from A[beta] oligomer toxicity.
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Análises de redes modulares de co-expressão gênica revelam vias importantes na doença de Alzheimer e paralisia supranuclear progressiva
… extent in AD, astrocytes, synaptic cells with glutamatergic neurons and myelination with oligodendrocytes. Finally, we show that genes identified as risk factors for AD or PSP are present in specific co-expression. Together, these results suggest that in the amyloidopathy model and in AD, …
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