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Showing 1 to 20 of 44 for “"glucuronic acid"”.
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Glucuronic Acid Patterning on Xylan in Arabidopsis thaliana
… and is decorated along its length by acetate and glucuronic acid (GlcA). It is likely that the position of these decorations, created by Trichome-Birefringence-Like (TBL) and Glucuronic acid substitution of Xylan (GUX) enzymes respectively, are key for the xylan molecule to perform a hypothesised …
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Biosynthesis and function of glucuronic acid substitution patterns on softwood xylan
… focuses on the biosynthesis and function of Glucuronic acid (GlcA) decorations on softwood xylan. Results demonstrate that this GUX (GlucUronic acid substitution of Xylan)-dependent xylan branching is critical for the maintenance of biomass recalcitrance in a model vascular plant Arabidopsis …
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The urinary excretion of conjugated glucuronic acid in healthy male volunteers
The amount of urinary conjugated glucuronic acid excreted by a free-living male population and the effect of certain factors, i.e. vegetable, fruit, meat, and charbroiled food intake, tobacco, alcohol, caffeine, and marijuana use, exposure to chemicals and familial cancer incidence, were …
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IDENTIFICATION OF THE ROLE OF [METHYL]GLUCURONIC ACID ON ARABINOGALACTAN POLYSACCHARIDES IN ARABIDOPSIS THALIANA
… and galactose, and minor sugars such as glucuronic acid (GlcA), fucose and xylose. The arabinogalactan polysaccharides do not decorate classical AGPs exclusively, but they can also be found decorating a wide range of proteins. Arabinogalactan proteins have been implicated in many …
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Crystallographic Studies of Cation-Glucuronic Acid Interactions: Structures of Calcium-Bromide Glucuronate and Sodium Glucuronate.
Calcium complexes of polysaccharides that contain D-glucuronate residues have been implicated in such biological processes as calcium storage, calcification, and calcium-dependent cell-cell adhesion. In 1957, Farber, Schubert and Schuster investigated the binding of sodium, potassium, and calcium …
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The Determination of Glucuronides in Biological Fluids by Lucigenin Chemiluminescence (beta-Glucuronidase, Glucuronic Acid, Immobilized Enzyme, (Hplc))
… (10,10'-dimethyl-9,9'-biacridinium nitrate) and glucuronic acid (an important organic reductant in humans) was studied. The main goal of this project was to develop an analytical method for the quantitation of conjugated glucuronic acid (i.e., glucuronides) in physiological fluids using this …
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Studies on Polysaccharide Synthesis and Solution Behavior
… interaction of water with hyaluronan, two uronic acids, beta-methyl glucuronic acid and beta-methyl galacturonic acid, were prepared, and their hydroxyl protons were studied using low temperature NMR. These results were compared to calculations of proton exchange in uronic acids. Calculations …
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Pathway and protein engineering for improved glucaric acid production in Escherichia coli
… the renewable production of chemicals. Glucaric acid was identified as a "top value added chemical from biomass" by the Department of Energy in 2004, and a biological route for its production from glucose in E. coli was developed in our lab in 2009. Two of the pathway enzymes, myo-inositol …
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The Substrate Specificity of Testicular Hyaluronidase and Its Use in Sequencing Chondroitin Sulfates
… to remove all nonreducing terminal D-glucuronic acid residues from oligosaccharides present in the reaction mixture. This action prevented the oligosaccharides from participating in the transglycosylation reaction. Conditions were also designed to eliminate sulfatase activities present …
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Molecular interactions of plant cell wall polymers
… are important. Previously, it was proposed that glucuronic acid side chains on xylan form ester bonds with lignin, and that this cross-linking might be important for cell wall properties. These bonds have been hypothesized to form if the glucuronic acid substitutions of xylan participate in …
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FROM GENE TO STRUCTURE: Formation of Iduronic Acid in Dermatan Sulfate by Two DS-epimerases
… unit consisting of N-acetylgalactosamine and glucuronic acid/iduronic acid. Formation of iduronic acid through epimerisation of glucuronic acid is a key modification in dermatan sulfate biosynthesis. In the present thesis two enzymes have been identified that catalyzes this reaction, i.e. …
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Toward the automated synthesis of carbohydrates and glycosaminoglycans
… use of glycosyl phosphates of glucosamine and glucuronic acid as competent glycosylating agents is illustrated. Further expansion of the glycosyl phosphates methodology to the construction of C-aryl and C-alkyl glycosides and resulted in the synthesis of the natural product 8,1 …
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Purification of uridine diphosphate glucuronyltransferase
… the conjugation of toxic compounds with glucuronic acid in reactions in which uridine diphosphate glucuronic acid (UDPGA) is the donor substrate. A new purification procedure for UDPGT has been developed. This procedure includes a Polyethylene glycol fractionation, ion exchange …
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The Role of Plant Chemical Defenses in The Nutritional Ecology of Voles
… showed that plant phenolics (quercetin, tannic acid, and quebracho) can reduce survival and growth of voles. The effects of phenolics were related to molecular structure, and included toxicity, feeding deterrence, and reduction of protein digestibility. Mammalian herbivores detoxicate many …
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Structural studies of the capsular polysaccharides from some strains of Klebsiella and Cryptococcus neoformans micro-organisms
… repeated oligosaccharide units containing -glucuronic acid and three to five hexose residues, with pyruvic acid ketal and 0-acetyl groups also present in some of the polysaccharides. Graded acid hydrolysis, monitored by gel-permeation chromato graphy, has been used to study the degradation …
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The Effect of Nitrates, pH, and Dissolved Inorganic Carbon Concentrations on the Extracellular Polysaccharide of Three Strains of Cyanobacteria Belonging to the Family Nostocaceae
… from biofilm growth conditions was reduced in glucuronic acid levels in both Anabaena variabilis ATCC 29413 and Anabaena PCC 7120. Under planktonic conditions, the glycan from A. variabilis contained glucuronic acid when grown in nitrate-free BG-11₀ medium whereas A. PCC 7120 produced similar …
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Comparison of Extraction Techniques for Δ-9-Tetrahydrocannabinol from Urine
… can be determined. Δ-9-THC is conjugated to glucuronic acid in urine and must be freed before the compound can be detected by chromatographic procedures. In past research, either a strong alkaline solution or β-glucuronidase has been used to cleave the bond between the Δ-9-THC and glucuronic …
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CHEMICAL SYNTHESIS AND BIOLOGICAL EVALUATION OF CHONDROITIN SULFATE DISACCHARIDES
… polysaccharides comprising of repeating uronic acid and amino sugar disaccharide units. Recently, it was shown that the sulfate groups present in chondroitin sulfate GAGs (CS) encode important functional information for the regulation of physiological processes. However, procurement of pure, …
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Xylan chain length and plant cell wall assembly
… residues, some with short branches of acetyls or glucuronic acid. Xylan coats cellulose, but is this coating important for the cell wall assembly? I used solid-state NMR to analyse cell walls of mutants with scarce and short xylan, i.e., with cellulose more uncoated. I found that shorter xylan …
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Untersuchungen zur Biosynthese der pflanzlichen Zellwand = [Identification and characterization of genes involved in plant cell wall synthesis]
… the UDP-rhamnose synthase (RHM), UDP-glucuronic acid epimerase (GAE) and the myo-inositol oxygenase (MIOX) families. For the three-membered RHM family relative ubiquitous expression was shown using variuos methods. For one of these genes, RHM2, T-DNA lines could be obtained. Moreover, …
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