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Showing 1 to 20 of 128 for “"glucosidase"”.
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Characterization of a Beta-glucosidase Aggregating Factor Responsible for the Null Beta-glucosidase Phenotype in Maize (Zea mays L.)
β-Glucosidase (β-D-glucoside glucohydrolase, EC 3.2.1.21) catalyzes the hydrolysis of aryl and alkyl β-D-glucosides as well as glucosides with a carbohydrate moiety such as cellobiose and other beta-linked oligosaccharides. In maize (Zea mays L.), β-glucosidase exists as 120 kD homodimers, but also …
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Purification and characterization of fungal β-glucosidase enzymes
… and one of the key enzyme group is β-glucosidases. These enzymatic systems are mainly adopted from wood-decaying fungi. The overall enzymatic system consists of different types of cellulases that first degrade the crystalline cellulose to oligosaccharides and cellobiose. In the final …
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Studies on maize beta glucosidase gene-enzyme system
Maize ß-glucosidase is implicated in phytohormone catabolism, disease resistance mechanisms, and also the catabolism of various ß-D-glucosides. The enzyme expressed in maize sporophytes is encoded by a highly polymorphic locus, Glul (chromosome 10). In the present study, maize ß-glucosidase was …
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Maize β-glucosidase substrate specificity and natural substrates
Plant β-glucosidases (β-D-glucoside glucohydrolases, E.C. 3.2.1.21) are known to function in defense in cyanogenic systems, and may also function in the metabolism of phytohormone glucosides and glucosides of other secondary plant products. Maize (Zea mays L.) β-glucosidase is a homodimer of 60 KD …
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Determination of the Binding Site and the Key Amino Acids on Maize β-Glucosidase Isozyme Glu1 Involved in Binding to β-Glucosidase Aggregating Factor (BGAF)
β-Glucosidase zymograms of certain maize genotypes (nulls) do not show any activity bands after electrophoresis. We have shown that a chimeric lectin called β-glucosidase aggregating factor (BGAF) is responsible for the absence of β-glucosidase activity bands on zymograms. BGAF specifically binds …
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Optimization of sequential purification of beta-glucosidase from tricoderma reesei in aqueous two-phase system
… for the purification of a valuable enzyme, beta-glucosidase, from microorganism Tricoderma reesei. The fungus T. reesei produces cellulose degrading enzymes, called cellulases: beta-glucosidase, endo-glucanase and exo-glucanase and low molecular weight proteins. For specific applications, the …
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The effects of the expression levels of cellobiose transporter and β-Glucosidase on ethanol fermentation
… enzymes such as cellobiose transporter and β-glucosidase, the ratios between those two enzymes can be a significant factor on cellobiose fermentation. In order to assess the effects of expression level of cellobiose transporter (CDT) and β-glucosidase (β-GL), in this study the copy number …
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Development, evaluation, and In-vitro assessment of artificial intelligence antidiabetic predictive models from α-glucosidase inhibitors
… accounting for over 90% of cases. Intestinal α-glucosidase enzymes, located at the small intestine's brush border, are important treatment targets in T2DM as they are involved in the terminal point of carbohydrate digestion in the gut, liberating glucose molecules, which are then transported …
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Isolation And Characterization Of Natural Alpha-Glucosidase Inhibitors From Antioxidant Rich Red Wine Grapes (vitis Vinifera)
… to exhibit an inhibitory activity against alpha- glucosidases. Alpha-glucosidases, in turn, play a major role in controlling starch digestion and therefore postprandial blood glucose, a target for diabetes management. </p> <p> <strong>Objective:</strong> This study aims to investigate the …
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Grape Extracts for Type 2 Diabetes Treatment Through Specific Inhibition of α-Glucosidase and Antioxidant Protection
… rich antioxidant grape extracts (GE) on α-glucosidase inhibitory activity in vitro and in vivo blood glucose control, oxidative stress, and inflammation associated with obesity-induced type 2 diabetes. Because intestinal α-glucosidase plays a key role in the digestion and absorption of …
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Evaluation of cocoa (Theobroma cacao) bean processing strategies to enhance alpha-glucosidase inhibitory activity of dietary cocoa
… (mDP). All CPEs dose-dependently inhibited α-glucosidase enzyme activity, with cool fermented/cool roasted cocoa powder exhibiting the best inhibition (IC50 of 62.2 µg/mL). Increasing flavanol mDP was correlated with decreasing IC50 values, suggesting that the complex flavanols produced during …
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A Comparative Study of Inductive Influence on the Hydrolysis of Beta-Thioglucopyranosides by Beta-Thioglucosidase and Beta-Glucosidase
… determine the absolute specificity of the .B-thioglucosidase and B-glucosidese enzyme systems toward the th1oglucosidic bond. The specificity of B-glucosidase of almond emulsin toward oxygen linkage seems to be absolute, since no hydrolysis was observed by this enzyme system toward the …
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Cloning and sequencing of a β-glucosidase of cDNA from Sorghum bicolor (L.) Moench and analysis of expression in seedlings
A full-length β-glucosidase encoding cDNA is isolated and sequenced from Sorghum bicolor (L) Moench. Using 5’- and 3’- end specific probes derived from the cDNA clone, the multiplicity of β-glucosidase genes and their expression in different tissues were studied. Southern blotting data showed that …
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Proteinaceous inhibitors of α-amylase and α-glucosidase from common bean (Phaseolus vulgaris L.): biochemical characterization and phylogenetic analysis of Sardinian cultivars
… proteinaceous α-amylase inhibitors (α-AIs) and α-glucosidase inhibitor could play an important role in weight loss and control of glycemic index above all in overweight and diabetic subjects. In this study 10 poorly studied Italian P. vulgaris cultivars were screened about their content in …
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Purification and characterisation of amylolytic enzymes from Lipomyces starkeyi
… developed for the extracellular carbohydrases; aglucosidase and a-amylase, secreted by the ascosporogenous soil yeast Lipomyces starkeyi NCYC 1436. Growth and optimum enzyme yield conditions were determined with cultures of L. starkeyi grown at 30°C on a medium containing 2% soluble starch, 1 % …
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Isolation and characterization of rice (Oryza sativa L.) β-Glucosidases
… purification and characterization of rice β-glucosidase, (2) determination of the physiological role of the enzyme during rice germination, and (3) histochemical localization of the enzyme. The method for partial purification of the enzyme was based on that of Schliemann (1984), which …
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PLASMA MEMBRANE ASSOCIATED GLYCOHYDROLASES: KINETICS PARAMETER CHARACTERIZATION AND BIOLOGICAL FUNCTION
… of beta-galactosidase, CBE sensitive beta-glucosidase, GBA2 beta-glucosidase, SMase and beta-hexosaminidases, enzymes working directly at the plasma membrane level using MUB derivates as substrate. Afterwards we applied this assays in order to study these PM associated activities in rat …
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